Novel mutations in Moloney Murine Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer
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[1] J. Jestin,et al. A population of thermostable reverse transcriptases evolved from Thermus aquaticus DNA polymerase I by phage display. , 2006, Angewandte Chemie.
[2] C. Malboeuf,et al. Thermal effects on reverse transcription: improvement of accuracy and processivity in cDNA synthesis. , 2001, BioTechniques.
[3] A. D. Clark,et al. Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA. , 1993, Proceedings of the National Academy of Sciences of the United States of America.
[4] G. Gerard,et al. Excess dNTPs minimize RNA hydrolysis during reverse transcription. , 2002, BioTechniques.
[5] A. Lever,et al. Pausing of reverse transcriptase on retroviral RNA templates is influenced by secondary structures both 5' and 3' of the catalytic site. , 1998, Nucleic acids research.
[6] G. Gerard,et al. The role of template-primer in protection of reverse transcriptase from thermal inactivation. , 2002, Nucleic acids research.
[7] D. Gelfand,et al. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. , 1991, Biochemistry.
[8] G. F. Joyce,et al. Randomization of genes by PCR mutagenesis. , 1992, PCR methods and applications.
[9] Anders Ståhlberg,et al. Comparison of reverse transcriptases in gene expression analysis. , 2004, Clinical chemistry.
[10] Nicholas M. Luscombe,et al. Amino acid?base interactions: a three-dimensional analysis of protein?DNA interactions at an atomic level , 2001, Nucleic Acids Res..
[11] Michael L. Kotewicz,et al. Isolation of cloned Moloney murine leukemia virus reverse transcriptase lacking ribonuclease H activity , 1988, Nucleic Acids Res..
[12] J. DeStefano,et al. Polymerization and RNase H activities of the reverse transcriptases from avian myeloblastosis, human immunodeficiency, and Moloney murine leukemia viruses are functionally uncoupled. , 1991, The Journal of biological chemistry.
[13] Samuel H. Wilson,et al. Structure/function studies of human immunodeficiency virus type 1 reverse transcriptase. Alanine scanning mutagenesis of an alpha-helix in the thumb subdomain. , 1994, The Journal of biological chemistry.
[14] S. Montaño,et al. Crystal structures of an N-terminal fragment from Moloney murine leukemia virus reverse transcriptase complexed with nucleic acid: functional implications for template-primer binding to the fingers domain. , 2000, Journal of molecular biology.
[15] H. Hogrefe,et al. Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit. , 2002, BioTechniques.
[16] N Sasaki,et al. Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA. , 1998, Proceedings of the National Academy of Sciences of the United States of America.
[17] D. Bolognesi,et al. Association of the viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. , 1971, Bibliotheca haematologica.
[18] P E Bourne,et al. Protein structure alignment by incremental combinatorial extension (CE) of the optimal path. , 1998, Protein engineering.
[19] G. Gerard,et al. Thermophilic bacterial DNA polymerases with reverse-transcriptase activity , 2004, Extremophiles.
[20] P. Holliger,et al. Directed evolution of DNA polymerase, RNA polymerase and reverse transcriptase activity in a single polypeptide. , 2006, Journal of molecular biology.
[21] T. Darden,et al. Reduced Frameshift Fidelity and Processivity of HIV-1 Reverse Transcriptase Mutants Containing Alanine Substitutions in Helix H of the Thumb Subdomain (*) , 1995, The Journal of Biological Chemistry.
[22] M. Georgiadis,et al. A directed approach to improving the solubility of Moloney murine leukemia virus reverse transcriptase , 2001, Protein science : a publication of the Protein Society.
[23] S. L. Berger,et al. First-strand cDNA synthesis primed with oligo(dT). , 1987, Methods in enzymology.
[24] Samuel H. Wilson,et al. A minor groove binding track in reverse transcriptase , 1997, Nature Structural Biology.
[25] N. Foulkes,et al. Reverse transcription of mRNA by Thermus aquaticus DNA polymerase. , 1989, Nucleic acids research.
[26] S. L. Berger,et al. Reverse transcriptase and its associated ribonuclease H: interplay of two enzyme activities controls the yield of single-stranded complementary deoxyribonucleic acid. , 1983, Biochemistry.
[27] K. Inouye,et al. Comparison of the thermal stabilities of reverse transcriptases from avian myeloblastosis virus and Moloney murine leukaemia virus. , 2008, Journal of biochemistry.
[28] A. Marx,et al. Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold. , 2006, Angewandte Chemie.
[29] M. Georgiadis,et al. The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus. , 2004, Structure.