Extraction optimization of inulinase obtained by solid state fermentation of Aspergillus ficuum JNSP5-06

Abstract Production of inulinase by solid state fermentation always involves an extraction step. In this study, the effects of the type of solvent, solid/liquid ratio, stirring rate, temperature, and extraction time on the recovery of inulinase from solid state fermentation of Aspergillus ficuum JNSP5-06 were investigated. After determining the best solvent, the extraction parameters for inulinase recovery were optimized by central composite rotatable design. The results showed that the maximum recovery of inulinase was obtained using 0.1 M sodium acetate of pH 4.5 as extraction solvent, in a solid/liquid ratio of 1:20.5, at 39.6 °C and 207 rpm for 40 min.

[1]  Ashok Pandey,et al.  Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianus , 1999 .

[2]  Zhenjiang Li,et al.  Medium optimization for the production of cyclic adenosine 3',5'-monophosphate by Microbacterium sp. no. 205 using response surface methodology. , 2009, Bioresource technology.

[3]  Marcio A. Mazutti,et al.  Optimization of inulinase production by solid-state fermentation using sugarcane bagasse as substrate , 2006 .

[4]  Wang Jin-hua,et al.  Optimization of solid-state medium for the production of inulinase by Kluyveromyces S120 using response surface methodology , 2007 .

[5]  M. Mazutti,et al.  Extraction of Inulinase Obtained by Solid State Fermentation of Sugarcane Bagasse by Kluyveromyces marxianus NRRL Y-7571 , 2008, Applied biochemistry and biotechnology.

[6]  L. Castilho,et al.  Production and extraction of pectinases obtained by solid state fermentation of agroindustrial residues with Aspergillus niger , 2000 .

[7]  M. Ramakrishna,et al.  Optimisation of downstream processing parameters for the recovery of pectinase from the fermented bran of Aspergillus carbonarius , 1999 .

[8]  M. Ayub,et al.  Extraction optimization of xylanases obtained by solid-state cultivation of Bacillus circulans BL53 , 2005 .

[9]  Ashok Pandey,et al.  Comparative studies on inulinase synthesis by Staphylococcus sp. and Kluyveromyces marxianus in submerged culture , 1999 .

[10]  Xueming Xu,et al.  Purification and characterisation of exo- and endo-inulinase from Aspergillus ficuum JNSP5-06 , 2009 .

[11]  A. Blandino,et al.  Evaluation of the conditions for the extraction of hydrolitic enzymes obtained by solid state fermentation from grape pomace , 2007 .

[12]  A. Pal,et al.  Production and extraction optimization of xylanase from Aspergillus niger DFR-5 through solid-state-fermentation. , 2010, Bioresource technology.

[13]  R. Banerjee,et al.  Some studies on optimization of extraction process for protease production in SSF , 1999 .

[14]  D. Mitchell,et al.  Leaching and characterization of Rhizopus oligosporus acid protease from solid-state fermentation , 1996 .

[15]  Francisco Maugeri,et al.  Optimization of inulinase production by Kluyveromyces marxianus using factorial design , 2001, Applied biochemistry and biotechnology.

[16]  J. Baratti,et al.  Purification, properties and comparison of invertase, exoinulinases and endoinulinases of Aspergillus ficuum , 1987, Applied Microbiology and Biotechnology.

[17]  Prabhjot Kaur Gill,et al.  Effect of media supplements and culture conditions on inulinase production by an actinomycete strain. , 2003, Bioresource technology.

[18]  Helen Treichel,et al.  Inulinase production by Kluyveromyces marxianus NRRL Y-7571 using solid state fermentation , 2006, Applied biochemistry and biotechnology.

[19]  S. Rhee,et al.  Fructose production from Jerusalem artichoke by inulinase immobilized on chitin , 1989, Biotechnology Letters.

[20]  B. C. Bhattacharyya,et al.  Protease extraction in solid state fermentation of wheat bran by a local strain of Rhizopus oryzae and growth studies by the soft gel technique , 2000 .

[21]  L. Castilho,et al.  Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger , 1999 .