Dynamic axial-position control of a laser-trapped particle by wave-front modification.

The axial position of a laser-trapped particle has been controlled by modification of the wave front by means of a membrane deformable mirror. The mirror gives wave-front modulation in terms of Zernike polynomials. By modulation of the Zernike defocus term we can modulate the particle position under conditions of laser trapping. A polystyrene particle of 1-microm diameter was moved along the optical axis direction for a distance of 2370 nm in minimum steps of 55.4 nm. We also demonstrated particle oscillation along the optical axis by changing the focal position in a sinusoidal manner. From the frequency dependency of the amplitude of particle oscillation we determined the spring constant as 91.7 nN/m.

[1]  E. Wolf,et al.  Principles of Optics (7th Ed) , 1999 .

[2]  Christoph F. Schmidt,et al.  Direct observation of kinesin stepping by optical trapping interferometry , 1993, Nature.

[3]  Satoshi Kawata,et al.  Surface-force measurement with a laser-trapped microprobe in solution , 2002 .

[4]  A. Ashkin,et al.  Optical trapping and manipulation of single cells using infrared laser beams , 1987, Nature.

[5]  T. Wilson,et al.  Aberration correction for confocal imaging in refractive‐index‐mismatched media , 1998 .

[6]  T. Yanagida,et al.  Movement of single myosin filaments and myosin step size on an actin filament suspended in solution by a laser trap. , 1994, Biophysical journal.

[7]  T. Wilson,et al.  Adaptive aberration correction in a confocal microscope , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[8]  R. Noll Zernike polynomials and atmospheric turbulence , 1976 .

[9]  P. C. Ke,et al.  Image enhancement in near-field scanning optical microscopy with laser-trapped metallic particles. , 1999, Optics letters.

[10]  W. Webb,et al.  Scanning-force microscope based on an optical trap. , 1993, Optics letters.

[11]  Jennifer E. Curtis,et al.  Dynamic holographic optical tweezers , 2002 .

[12]  Y Fainman,et al.  Wave-front generation of Zernike polynomial modes with a micromachined membrane deformable mirror. , 1999, Applied optics.

[13]  S. Chu,et al.  Observation of a single-beam gradient force optical trap for dielectric particles. , 1986, Optics letters.

[14]  S. Kawata,et al.  Gold-bead scanning near-field optical microscope with laser-force position control. , 1997, Optics letters.

[15]  S Kawata,et al.  Fluorescence imaging with a laser trapping scanning near‐field optical microscope , 1999, Journal of microscopy.

[16]  H. Ozaktas,et al.  Fractional Fourier transform as a tool for analyzing beam propagation and spherical mirror resonators. , 1994, Optics letters.

[17]  M. Schliwa,et al.  Calibration of light forces in optical tweezers. , 1995, Applied optics.

[18]  S. Quake,et al.  Relaxation of a single DNA molecule observed by optical microscopy. , 1994, Science.