Contrast-enhanced photoacoustic imaging with an optical wavelength of 1064 nm

Photoacoustic (PA) imaging is a biomedical imaging method that can provide both structural and functional information of living tissues beyond the optical diffusion limit by combining the concepts of conventional optical and ultrasound imaging methods. Although endogenous chromophores can be utilized to acquire PA images of biological tissues, exogenous contrast agents that absorb near-infrared (NIR) lights have been extensively explored to improve the contrast and penetration depth of PA images. Here, we demonstrate Bi2Se3 nanoplates, that strongly absorbs NIR lights, as a contrast agent for PA imaging. In particularly, the Bi2Se3 nanoplates produce relatively strong PA signals with an optical wavelength of 1064 nm, which has several advantages for deep tissue imaging including: (1) relatively low absorption by other intrinsic chromophores, (2) cost-effective light source using Nd:YAG laser, and (3) higher available energy than other NIR lights according to American National Standards Institute (ANSI) safety limit. We have investigated deep tissue imaging capability of the Bi2Se3 nanoplates by acquiring in vitro PA images of microtubes under chicken breast tissues. We have also acquired in vivo PA images of bladders, gastrointestinal tracts, and sentinel lymph nodes in mice after injection of the Bi2Se3 nanoplates to verify their applicability to a variety of biomedical research. The results show the promising potential of the Bi2Se3 nanoplates as a PA contrast agent for deep tissue imaging with an optical wavelength of 1064 nm.

[1]  Lihong V. Wang,et al.  In vivo photoacoustic tomography of chemicals: high-resolution functional and molecular optical imaging at new depths. , 2010, Chemical reviews.

[2]  Todd N. Erpelding,et al.  Deeply penetrating in vivo photoacoustic imaging using a clinical ultrasound array system , 2010, Biomedical optics express.

[3]  M. C. Mancini,et al.  Bioimaging: second window for in vivo imaging. , 2009, Nature nanotechnology.

[4]  Liang Song,et al.  Dual-color photoacoustic lymph node imaging using nanoformulated naphthalocyanines. , 2015, Biomaterials.

[5]  Paul Kumar Upputuri,et al.  Broadband Absorbing Semiconducting Polymer Nanoparticles for Photoacoustic Imaging in Second Near-Infrared Window. , 2017, Nano letters.

[6]  Chulhong Kim,et al.  Biodegradable Photonic Melanoidin for Theranostic Applications. , 2016, ACS nano.

[7]  Wei Long,et al.  Metabolizable Bi2Se3 Nanoplates: Biodistribution, Toxicity, and Uses for Cancer Radiation Therapy and Imaging , 2013, 1312.1773.

[8]  Chulhong Kim,et al.  Multiplane spectroscopic whole-body photoacoustic imaging of small animals in vivo , 2014, Medical & Biological Engineering & Computing.

[9]  Chulhong Kim,et al.  Biodegradable Nitrogen-Doped Carbon Nanodots for Non-Invasive Photoacoustic Imaging and Photothermal Therapy , 2016, Theranostics.

[10]  Chulhong Kim,et al.  A Multifunctional Subphthalocyanine Nanosphere for Targeting, Labeling, and Killing of Antibiotic-Resistant Bacteria. , 2015, Angewandte Chemie.

[11]  Lihong V. Wang,et al.  A practical guide to photoacoustic tomography in the life sciences , 2016, Nature Methods.

[12]  Timothy R. Cook,et al.  A Phosphorus Phthalocyanine Formulation with Intense Absorbance at 1000 nm for Deep Optical Imaging , 2016, Theranostics.

[13]  Chulhong Kim,et al.  Opportunities for Photoacoustic-Guided Drug Delivery. , 2015, Current drug targets.

[14]  Jesse V. Jokerst,et al.  Semiconducting Polymer Nanoparticles as Photoacoustic Molecular Imaging Probes in Living Mice , 2014, Nature nanotechnology.

[15]  Chulhong Kim,et al.  Multimodal Photoacoustic Tomography , 2013, IEEE Transactions on Multimedia.

[16]  Chulhong Kim,et al.  Programmable Real-time Clinical Photoacoustic and Ultrasound Imaging System , 2016, Scientific Reports.

[17]  Mukund Seshadri,et al.  Non-invasive, Multimodal Functional Imaging of the Intestine with Frozen Micellar Naphthalocyanines , 2014, Nature nanotechnology.

[18]  Chulhong Kim,et al.  Sentinel lymph nodes and lymphatic vessels: noninvasive dual-modality in vivo mapping by using indocyanine green in rats--volumetric spectroscopic photoacoustic imaging and planar fluorescence imaging. , 2010, Radiology.

[19]  Chulhong Kim,et al.  Nanostructured Phthalocyanine Assemblies with Protein-Driven Switchable Photoactivities for Biophotonic Imaging and Therapy. , 2017, Journal of the American Chemical Society.

[20]  Chulhong Kim,et al.  "Smart" gold nanoparticles for photoacoustic imaging: an imaging contrast agent responsive to the cancer microenvironment and signal amplification via pH-induced aggregation. , 2016, Chemical communications.