论文信息 - Convenient purification of human spleen fibrinolytic proteinase (SFP) and human leucocyte elastase by affinity chromatography.

Convenient purification of human spleen fibrinolytic proteinase (SFP) and human leucocyte elastase by affinity chromatography.

A peptide inhibitor, Suc-L-Tyr-D-Leu-D-Val-pNA, for human spleen fibrinolytic proteinase (SFP) and for human leucocyte elastase was coupled with AH Sepharose 4B using water soluble carbodiimide. A 116-fold purification of the crude extract of SFP and a 59-fold purification of the partially purified leucocyte elastase were achieved by (Suc-L-Tyr-D-Leu-D-Val-pNA)-AH Sepharose 4B affinity chromatography.

Y. Tsuda | Y. Okada | U. Okamoto | Y. Nagamatsu

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