Love wave based portable sensing system for on-line detection of carcinoembryonic antigen in exhaled breath condensate

Lung cancer is continuously the leading cause of cancer related death. Noninvasive exhaled breath condensate (EBC) detection using portable devices may pave a new avenue for monitoring conditions of the high-risk population of lung cancer, thereby increasing the 5-year survival rate. Here, a portable system is proposed for on-line detection of carcinoembryonic antigen (CEA) in EBC. This miniaturized system employs an aptameric Love-wave sensor to provide a detection with advantages of low cost, operational simplicity and high sensitivity. In addition, the aptasensor can be used in label-free and real-time monitoring. Integrating with measurement circuits and iOS APP, the system realizes online transmission and processing of signals which reflect the CEA concentration. Artificial samples have been detected, and experimental results demonstrate that the present system is sensitive (limit of detection = 1 ng/ml) and accurate (relative accuracy = 0.995). Applying in detection of clinical EBC, the relative error is as low as 0.305% and the result can be used to distinguish lung cancer patients from healthy subjects. Therefore, this portable system offers a potential solution for noninvasive detection of lung cancer in its early stage.

[1]  A. Jemal,et al.  Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries , 2018, CA: a cancer journal for clinicians.

[2]  Wei Wen,et al.  Novel electrochemical aptamer biosensor based on gold nanoparticles signal amplification for the detection of carcinoembryonic antigen , 2013 .

[3]  Bastian E. Rapp,et al.  Surface acoustic wave biosensors: a review , 2008, Analytical and bioanalytical chemistry.

[4]  Ping Wang,et al.  A miniaturized immunosensor platform for automatic detection of carcinoembryonic antigen in EBC , 2014 .

[5]  M. Mascini.  Aptamers and their applications , 2008, Analytical and bioanalytical chemistry.

[6]  Yan Su,et al.  A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe , 2015, Sensors.

[7]  Yvonne Joseph,et al.  Aptamer-Based Biosensors for Antibiotic Detection: A Review , 2018, Biosensors.

[8]  Jun Kondoh,et al.  Real-time monitoring of methanol concentration using a shear horizontal surface acoustic wave sensor for direct methanol fuel cell without reference liquid measurement , 2017 .

[9]  Yingfu Li,et al.  Nucleic acid aptamers and enzymes as sensors. , 2006, Current opinion in chemical biology.

[10]  Eiko Ueno,et al.  Reproducibility of scan prescription in follow-up brain MRI: manual versus automatic determination , 2013, Radiological Physics and Technology.

[11]  Dinesh K. Aswal,et al.  Ultrasensitive and selective detection of dopamine using cobalt-phthalocyanine nanopillar-based surface acoustic wave sensor. , 2014, ACS applied materials & interfaces.

[12]  E. Mitchell,et al.  Carcinoembryonic Antigen in the Staging and Follow-up of Patients with Colorectal Cancer , 2005, Cancer investigation.

[13]  Yan Su,et al.  Theoretical Study of Monolayer and Double-Layer Waveguide Love Wave Sensors for Achieving High Sensitivity , 2017, Sensors.

[14]  J. Hunt,et al.  Exhaled breath condensate: an evolving tool for noninvasive evaluation of lung disease. , 2002, The Journal of allergy and clinical immunology.

[15]  Franz L. Dickert,et al.  Surface Acoustic Wave (SAW) for Chemical Sensing Applications of Recognition Layers † , 2017, Sensors.

[16]  Laurent Francis,et al.  Love Wave Biosensors: A Review , 2013 .

[17]  T R Fleming,et al.  An evaluation of the carcinoembryonic antigen (CEA) test for monitoring patients with resected colon cancer. , 1993, JAMA.

[18]  Graeme P Currie,et al.  Variation in lung cancer survival rates between countries: do differences in data reporting contribute? , 2006, Respiratory medicine.

[19]  K. Lewis,et al.  A review of novel biological tools used in screening for the early detection of lung cancer , 2009, Postgraduate Medical Journal.

[20]  J. Sørensen,et al.  Carcinoembryonic antigen (CEA) as tumor marker in lung cancer. , 2012, Lung cancer.

[21]  M. Shamsipur,et al.  A review: Aptamer-based analytical strategies using the nanomaterials for environmental and human monitoring of toxic heavy metals. , 2017, Talanta.

[22]  M. Ward,et al.  In Situ Interfacial Mass Detection with Piezoelectric Transducers , 1990, Science.

[23]  Cong Zhao,et al.  CEA, SCC and NSE levels in exhaled breath condensate—possible markers for early detection of lung cancer , 2013, Journal of breath research.

[24]  Ping Wang,et al.  A point-of-care testing system with Love-wave sensor and immunogold staining enhancement for early detection of lung cancer , 2014, Biomedical microdevices.

[25]  Ping Wang,et al.  Sensitive detection of carcinoembryonic antigen in exhaled breath condensate using surface acoustic wave immunosensor , 2015 .

[26]  Jun Ou-Yang,et al.  Self-biased vector magnetic sensor based on a Love-type surface acoustic wave resonator , 2018 .