Substrate inhibition kinetics: Phenol degradation by Pseudomonas putida

A pure culture of Pseudoinonas putida was grown in both a batch and continuous culture using phenol as the limiting substrate. Of two substrate inhibition models examined, the Haldane function was found to statistically best describe the kinetics. The applicable kinetic constants were either measured (μM, KI) or estimated (KS) from the experimental data. Particularly in the continuous culture, wall growth was found to exert significant effects on the broth biomass concentration and phenol conversion, both of which decreased with increasing amounts of wall growth. These effects are opposite to those predicted by wall growth models and to experimental results of others using mixed culture (activated sludge) systems.

[1]  W. Evans.  Oxidation of phenol and benzoic acid by some soil bacteria. , 1947, The Biochemical journal.

[2]  K. Laidler.  The chemical kinetics of enzyme action , 1973 .

[3]  J. Monod,et al.  Recherches sur la croissance des cultures bactériennes , 1942 .

[4]  V H Edwards,et al.  The influence of high substrate concentrations on microbial kinetics , 1970, Biotechnology and bioengineering.

[5]  S. Pirt.  The maintenance energy of bacteria in growing cultures , 1965, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[6]  J. Fawell,et al.  The acute toxicity of phenol to rainbow trout in saline waters , 1967 .

[7]  G. Hamer,et al.  Effect of wall growth in steady‐state continuous cultures , 1971 .

[8]  F. Jansen,et al.  Substrate Inhibition of the Growth of Bacterium ncib 8250 by Phenol , 1973 .

[9]  J. Howell,et al.  Mixed culture biooxidation of phenol. III. Existence of multiple steady states in continuous culture with wall growth , 1973 .

[10]  J. Howell,et al.  Effect of wall growth on scale‐up problems and dynamic operating characteristics of the biological reactor , 1972 .

[11]  V. Brown,et al.  The effect of temperature on the acute toxicity of phenol to rainbow trout in hard water , 1967 .

[12]  R. Bayly,et al.  Metabolism of Phenol and Cresols by Mutants of Pseudomonas putida , 1973, Journal of bacteriology.

[13]  R. Stanier.  The Oxidation of Aromatic Compounds by Fluorescent Pseudomonads , 1948, Journal of bacteriology.

[14]  Toshimasa Yano,et al.  Dynamic behavior of the chemostat subject to substrate inhibition , 1969 .

[15]  J. Howell,et al.  Mixed culture biooxidation of phenol. I. Determination of kinetic parameters , 1973 .