Development of multi-organs-on-a-chip mimicking physiological parameters: Evaluation of the function using prodrug CPT-11

In this study, we developed a microfluidic device with metabolism function during pharmacokinetic study. We reproduced physiological parameters such as an organ volume ratio and a blood flow ratio on the device. The ratios are replicated by area of cell culture chambers and a bypass channel. HepG2 (Metabolism Model) and A549 (Target Cancer Cells) were co-cultured in each culture chambers in the device. Then we performed cell-based assays using an anticancer drug model (CPT-11) and an antihyperlipidemic drug model (Simvastatin). As a result, A549 cells density in the case of CPT-11 and Simvastatin exposure decreased due to inhibit Carboxylesterase metabolism by Simvastatin. We conclude that the device can be applied to the evaluation of complicated metabolism function in human body.