Direct current electrical stimulation chamber for treating cells in vitro.

Electrical stimulation has been shown to promote healing and regeneration in skin, bone, muscle, and nerve tissues in clinical studies. Recently, studies applying electrical stimulation to influence cell behavior associated with proliferation, differentiation, and migration have provided a better understanding of the underlying mechanisms of electrical stimulation-based clinical treatments and improved tissue-engineered products through electro-bioreactor technologies. Here, we present a novel device for delivering direct current (DC) electrical stimulation (ES) to cultivated cells in vitro. Our simplified electro-bioreactor is customized for applying DC electrical current simultaneously in six individual tissue culture wells. The design overcomes previous experimental replicate limitations, thus reducing experimental time and cost.

[1]  Simon P Hoerstrup,et al.  Cell therapy, 3D culture systems and tissue engineering for cardiac regeneration. , 2014, Advanced drug delivery reviews.

[2]  R. Nuccitelli,et al.  Endogenous ionic currents and DC electric fields in multicellular animal tissues. , 1992, Bioelectromagnetics.

[3]  Min Zhao,et al.  Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo , 2006, Nature Protocols.

[4]  R. Borgens.  Endogenous ionic currents traverse intact and damaged bone. , 1984, Science.

[5]  A. Caplan.  Review: mesenchymal stem cells: cell-based reconstructive therapy in orthopedics. , 2005, Tissue engineering.

[6]  C. McCaig,et al.  Electrical dimensions in cell science , 2009, Journal of Cell Science.

[7]  Paolo Giannoni,et al.  Tissue engineering and cell therapy of cartilage and bone. , 2003, Matrix biology : journal of the International Society for Matrix Biology.

[8]  A. Bayat,et al.  Electrical Stimulation in Bone Healing: Critical Analysis by Evaluating Levels of Evidence , 2011, Eplasty.

[9]  J. Cooper-White,et al.  Effects of electric fields on human mesenchymal stem cell behaviour and morphology using a novel multichannel device. , 2015, Integrative biology : quantitative biosciences from nano to macro.

[10]  Min Zhao,et al.  Controlling cell behavior electrically: current views and future potential. , 2005, Physiological reviews.

[11]  Mark Cronin-Golomb,et al.  Osteoblastic differentiation and stress response of human mesenchymal stem cells exposed to alternating current electric fields , 2011, Biomedical engineering online.

[12]  C. McCaig,et al.  Physiological electrical fields modify cell behaviour. , 1997, BioEssays : news and reviews in molecular, cellular and developmental biology.