A novel ferrocene encapsulated palladium-linked ormosil-based electrocatalytic dopamine biosensor

Abstract A novel finding on the development of electrocatalytic biosensor for dopamine is reported. The new electrocatalytic dopamine biosensor is developed using ferrocene encapsulated palladium (Pd)-linked organically-modified sol–gel glass (ormosil). The alkoxy precursors used for the preparation of new ormosil-based electrocatalytic biosensor are palladium-linked glycidoxypropyltrimethoxysilane and trimethoxysilane. The optimum concentrations of these precursors are added in aqueous solution of ferrocene monocarboxylic acid and HCl followed by gelation for 30 h at 25°C to form ormosil. The ferrocene encapsulated ormosil is characterized based on cyclic voltammetric measurements. The CV results shows peak separation of 57–59 mV and a linear relation between peak current and square root of scan rate suggesting well behaved reversible electrochemistry of ormosil encapsulated ferrocene. The CV results and the detection of ferrocene in working medium shows that ferrocene is not leached out of ormosil matrix. The tyrosinase is immobilized within polyvinyl alcohol over the ferrocene encapsulated new ormosil and finally mounted using nucleopore membrane. The electrocatalytic response of immobilized tyrosinase over new ormosil is observed and the results are reported. The performance, stability, and reproducibility of new ormosil-based dopamine biosensor are reported.

[1]  P. Audebert,et al.  Modified electrodes from organic-inorganic hybrid gels containing ferrocene units covalently bonded inside a silica network , 1994 .

[2]  Jyh-Myng Zen and,et al.  A Selective Voltammetric Method for Uric Acid and Dopamine Detection Using Clay-Modified Electrodes , 1997 .

[3]  Akira Fujishima,et al.  Electroanalysis of dopamine and NADH at conductive diamond electrodes , 1999 .

[4]  Horiuchi,et al.  Subnanoliter volume wall-jet cells combined with interdigitated microarray electrode and enzyme modified planar microelectrode , 2000, Analytical chemistry.

[5]  C. Rausch,et al.  Electroactivity of Redox Probes Encapsulated within Sol-Gel-Derived Silicate Films , 1997 .

[6]  S. Upadhyay,et al.  Studies on Ferrocene Immobilized Sol‐Gel Glasses and Its Application in the Construction of a Novel Solid‐State Ion Sensor , 1999 .

[7]  Satyanshu K. Upadhyay,et al.  A new glucose sensor based on encapsulated glucose oxidase within organically modified sol–gel glass , 1999 .

[8]  T. Tan,et al.  Dopamine sensing and selectivity of Nafion-coated plant tissue powder sensors. , 1995, Talanta.

[9]  S. Sampath,et al.  Sol−Gel Materials in Electrochemistry , 1997 .

[10]  O. Lev,et al.  Voltammetric studies of composite ceramic carbon working electrodes , 1994 .

[11]  E. Hall,et al.  An electrochemically grown polymer as an immobilisation matrix for whole cells: Application in an amperometric dopamine Sensor , 1990 .

[12]  Bruce Dunn,et al.  Biomolecular materials based on sol-gel encapsulated proteins , 1994 .

[13]  T. Tan,et al.  Dopamine-sensing efficacy and characteristics of pretreated plant tissue powder sensors , 1995 .

[14]  P. Audebert,et al.  Electrochemical probing of the activity of glucose oxidase embedded sol-gel matrixes , 1993 .

[15]  S. Sampath,et al.  Inert metal-modified, composite ceramic-carbon, amperometric biosensors:  renewable, controlled reactive layer. , 1996, Analytical chemistry.

[16]  P. Audebert,et al.  Modified electrodes from organic-inorganic hybrid gels formed by hydrolysis-polycondensation of some trimethoxysilylferrocenes , 1996 .

[17]  Juan Li,et al.  Reagentless Amperometric Determination of Hydrogen Peroxide by Silica Sol–Gel Modified Biosensor , 1997 .

[18]  J Wang,et al.  Screen-printable sol-gel enzyme-containing carbon inks. , 1996, Analytical chemistry.

[19]  T. Tan,et al.  Selectivity enhancement of an immobilized apple powder enzymatic sensor for dopamine. , 1994, Biosensors & bioelectronics.

[20]  M. Yokoyama,et al.  Applications of sol-gel-derived membranes to neutral carrier-type ion-sensitive field-effect transistors. , 1997, Analytical Chemistry.

[21]  S. Upadhyay,et al.  A New Glucose Biosensor Based on Sandwich Configuration of Organically Modified Sol-Gel Glass , 1999 .

[22]  S. Cosnier,et al.  An electrochemical method for making enzyme microsensors. Application to the detection of dopamine and glutamate. , 1997, Analytical chemistry.

[23]  J S Valentine,et al.  Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method. , 1992, Science.

[24]  G. Rivas,et al.  Amperometric determination of dopamine on vegetal-tissue enzymatic electrodes. Analysis of interferents and enzymatic selectivity , 1997 .

[25]  Ovadia Lev,et al.  Sol-gel vanadium pentaoxide glucose biosensor , 1993 .

[26]  Joseph Wang,et al.  Sol‐gel‐derived metal‐dispersed carbon composite amperometric biosensors , 1997 .

[27]  T. Park.  Amperometric Determination of Hydrogen Peroxide by Utilizing a Sol-Gel-Derived Biosensor Incorporating an Osmium Redox Polymer as Mediator , 1999 .

[28]  P. Pandey,et al.  Studies on Glucose Biosensors Based on Nonmediated and Mediated Electrochemical Oxidation of Reduced Glucose Oxidase Encapsulated Within Organically Modified Sol‐Gel Glasses , 1999 .

[29]  J. Wang,et al.  Sol-gel-derived gold composite electrodes. , 1997, Analytical chemistry.

[30]  O. Lev,et al.  Wiring of Glucose Oxidase to Carbon Matrices VIA Sol-Gel Derived Redox Modified Silicate , 1996 .