论文信息 - Ability of the T cell‐replacing polyanion dextran sulfate to trigger the alternate pathway of complement activation

Ability of the T cell‐replacing polyanion dextran sulfate to trigger the alternate pathway of complement activation

Dextran sulfate (DS) consumed C3 in C4 deficient guinea pig serum. This temperature‐dependent reaction required Mg++ ions and could therefore be blocked by EDTA. Isolated C3 was not influenced by DS, but serum factors were required for C3 consumption. The C3 proactivator as well as C3 were converted to their activated state by DS in guinea pig and human serum, as revealed by immunoelectrophoretical analysis. DS generated anaphylatoxin activity in serum. It is concluded that DS activates C3 via the alternate pathway of complement activation. This potency of the polyanion might serve as a tentative explanation for its T cell‐replacing effect in an antibody‐forming system, which was reported by Diamantstein and his coworkers.

[1] K. Hartmann,et al. Bound C3 as the second signal for B-cell activation , 1973 .

[2] W. König,et al. Analysis of bypass activation of C3 by endotoxic LPS and loss of this potency. , 1973, Immunology.

[3] S. Ruddy,et al. Alternate complement pathway: factors involved in cobra venom factor (CoVF) activation of the third component of complement (C3). , 1973, Journal of immunology.

[4] W. König,et al. Bypass-activation of the complement system starting with C3. I. Generation and function of an enzyme from a factor of guinea-pig serum and cobra venom. , 1972, Immunology.

[5] O. Götze,et al. THE C3-ACTIVATOR SYSTEM: AN ALTERNATE PATHWAY OF COMPLEMENT ACTIVATION , 1971, The Journal of experimental medicine.

[6] D. Bitter‐Suermann,et al. Independent and consecutive action of the complement components C5, C6 and C7 in immune hemolysis. I. Preparation of EAC1-5 with purified guinea pig C3 and C5. , 1970, Immunochemistry.

[7] I. Green,et al. Genetically Controlled Total Deficiency of the Fourth Component of Complement in the Guinea Pig , 1970, Science.

[8] T. Diamantstein,et al. Stimulation of humoral antibody formation by polyanions. III. Restoration of the immune response to sheep red blood cells by polyanions in thymectomized and lethally irradiated mice protected with bone marrow cells , 1971 .