A carbon fiber microelectrode-based third-generation biosensor for superoxide anion.

Implantable and miniature carbon fiber microelectrode (CFME)-based third-generation biosensor for superoxide anion (O(2)(-)) was fabricated for the first time. The CFME-based biosensor was constructed by electro-deposition of Au nanoparticles on the CFMEs and then modification of the Au nanoparticles by cysteine followed by immobilization of superoxide dismutase (SOD) on the electrodes. The direct electrochemistry of the SOD immobilized on the CFME-based electrodes was efficiently realized by electron transfer promoter - cysteine molecules confined on the Au nanoparticles deposited on the CFMEs. The CFME-based biosensors were demonstrated to possess striking analytical properties for O(2)(-) determination, such as optional operation potentials, high selectivity and sensitivity as well as good stability. Along with the implantable capacity inherent in the CFMEs, these striking analytical properties of the CFME-based biosensors substantially make them potential for in vivo determination of O(2)(-).

[1]  T. Ohsaka,et al.  A facilitated electron transfer of copper--zinc superoxide dismutase (SOD) based on a cysteine-bridged SOD electrode. , 2002, Biochimica et biophysica acta.

[2]  T. Iizuka,et al.  Direct measurement of oscillatory generation of superoxide anions by single phagocytes , 1996, FEBS letters.

[3]  Continuous on-line measurement of cerebral hydrogen peroxide using enzyme-modified ring-disk plastic carbon film electrode. , 2002, Analytical chemistry.

[4]  G. Benzi,et al.  Age- and peroxidative stress-related modifications of the cerebral enzymatic activities linked to mitochondria and the glutathione system. , 1995, Free radical biology & medicine.

[5]  Y. Muto,et al.  Amperometric determination of superoxide anions generated from phytoplankton Chattonella antiqua , 1992 .

[6]  L. Persi,et al.  New biosensor for superoxide radical used to evidence molecules of biomedical and pharmaceutical interest having radical scavenging properties. , 2000, Journal of pharmaceutical and biomedical analysis.

[7]  S. Descroix,et al.  Evaluation of the Selectivity of Overoxidized Polypyrrole/Superoxide Dismutase Based Microsensor for the Electrochemical Measurement of Superoxide Anion in Solution , 2001 .

[8]  T. Iizuka,et al.  Electrochemical determination of superoxide anions generated from a single neutrophil , 1991 .

[9]  M. Finot,et al.  Characterization of n-alkanethiolate monolayers adsorbed to electrochemically deposited gold nanocrystals on glassy carbon electrodes , 2000 .

[10]  Barry Halliwell,et al.  Reactive Oxygen Species and the Central Nervous System , 1992, Journal of neurochemistry.

[11]  T. Ohsaka,et al.  A SOD-Based Amperometric Biosensor for Superoxide Ion , 2002 .

[12]  T. Ohsaka,et al.  An extraordinary electrocatalytic reduction of oxygen on gold nanoparticles-electrodeposited gold electrodes ☆ , 2002 .

[13]  T. Malinski,et al.  Preparation and optimization of superoxide microbiosensor , 1998 .

[14]  G. Poli,et al.  Free Radicals: from Basic Science to Medicine , 1993 .

[15]  I. Fridovich,et al.  Superoxide from glucose oxidase or from nitroblue tetrazolium? , 1995, Archives of biochemistry and biophysics.

[16]  E. Bassenge,et al.  Detection of superoxide radicals and peroxynitrite by 1-hydroxy-4-phosphonooxy-2,2,6,6-tetramethylpiperidine: quantification of extracellular superoxide radicals formation. , 1998, Biochemical and biophysical research communications.

[17]  M. Tarpey,et al.  Chemiluminescent detection of oxidants in vascular tissue. Lucigenin but not coelenterazine enhances superoxide formation. , 1999, Circulation research.

[18]  E. Laviron.  General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems , 1979 .

[19]  J. Cooper,et al.  Direct, real-time sensing of free radical production by activated human glioblastoma cells. , 1998, Free radical biology & medicine.

[20]  K. Vengatajalabathy Gobi,et al.  Efficient mediatorless superoxide sensors using cytochrome c-modified electrodes : surface nano-organization for selectivity and controlled peroxidase activity , 2000 .

[21]  M. Porter,et al.  The electrochemical desorption of n-alkanethiol monolayers from polycrystalline Au and Ag electrodes , 1991 .

[22]  J. Joseph,et al.  Detection of superoxide anion using an isotopically labeled nitrone spin trap: potential biological applications , 2000, FEBS letters.

[23]  T. Ohsaka,et al.  A superoxide dismutase-modified electrode that detects superoxide ion. , 2002, Chemical communications.

[24]  Jonathan M. Cooper,et al.  Direct electron transfer reactions between immobilized cytochrome c and modified gold electrodes , 1993 .

[25]  K. Pritchard,et al.  Superoxide anion formation from lucigenin: an electron spin resonance spin‐trapping study , 1997, FEBS letters.

[26]  S. Pietri,et al.  5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide: a new efficient phosphorylated nitrone for the in vitro and in vivo spin trapping of oxygen-centered radicals. , 1995, Journal of medicinal chemistry.

[27]  F. Xu,et al.  Miniaturized amperometric biosensor based on xanthine oxidase for monitoring hypoxanthine in cell culture media. , 2001, Analytical biochemistry.

[28]  T. Ohsaka,et al.  Electrochemical Reduction of Oxygen on Gold Nanoparticle-Electrodeposited Glassy Carbon Electrodes , 2003 .

[29]  T. Ohsaka,et al.  Superoxide dismutase-based third-generation biosensor for superoxide anion. , 2002, Analytical chemistry.

[30]  L. Mao,et al.  Amperometric Biosensor for Glutathione Based on Osmium‐Polyvinylpyridine Gel Polymer and Glutathione Sulfhydryl Oxidase , 2000 .

[31]  I. Fridovich,et al.  Lucigenin (bis-N-methylacridinium) as a mediator of superoxide anion production. , 1997, Archives of biochemistry and biophysics.

[32]  D. Harrison,et al.  Hypercholesterolemia increases endothelial superoxide anion production. , 1993, The Journal of clinical investigation.

[33]  L. Mao,et al.  Electrochemical Microsensor for In Vivo Measurements of Oxygen Based on Nafion and Methylviologen Modified Carbon Fiber Microelectrode , 1999 .

[34]  D. Bagchi,et al.  Oxidative mechanisms in the toxicity of metal ions. , 1995, Free radical biology & medicine.

[35]  J. Cooper,et al.  Electrochemical sensors for direct reagentless measurement of superoxide production by human neutrophils. , 1992, Free radical research communications.

[36]  F. Hamada,et al.  Fluorescent molecular sensory system based on bis pyrene-modified gamma-cyclodextrin dimer for steroids and endocrine disruptors. , 2001, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[37]  J. Devynck,et al.  Superoxide release from interleukin-1B-stimulated human vascular cells: in situ electrochemical measurement. , 1999, Free radical biology & medicine.

[38]  M. Weisfeldt,et al.  Direct measurement of free radical generation following reperfusion of ischemic myocardium , 1987 .

[39]  T. Ohyashiki,et al.  Detection of superoxide anion radical in phospholipid liposomal membrane by fluorescence quenching method using 1,3-diphenylisobenzofuran. , 1999, Biochimica et biophysica acta.

[40]  T. Ohsaka,et al.  Fabrication of Au(111)-Like Polycrystalline Gold Electrodes and Their Applications to Oxygen Reduction , 2004 .

[41]  T. Ohsaka,et al.  Self-assembled monolayers suitable for electron-transfer promotion of copper, zinc-superoxide dismutase , 2004 .

[42]  Mark T. McDermott,et al.  Characterization of electrochemically deposited gold nanocrystals on glassy carbon electrodes , 1999 .

[43]  P. Osborne,et al.  Plastic film carbon electrodes: enzymatic modification for on-line, continuous, and simultaneous measurement of lactate and glucose using microdialysis sampling. , 1998, Analytical chemistry.

[44]  C. Tolias,et al.  Superoxide generation from constitutive nitric oxide synthase in astrocytes in vitro regulates extracellular nitric oxide availability. , 1999, Free radical biology & medicine.

[45]  S. Grunfeld,et al.  Electrochemical determination of superoxide and nitric oxide generated from biological samples , 1998 .

[46]  S. Gross,et al.  Nitric oxide: pathophysiological mechanisms. , 1995, Annual review of physiology.

[47]  Wolfgang Schuhmann,et al.  Electron transfer principles in amperometric biosensors: direct electron transfer between enzymes and electrode surface , 1996 .

[48]  Zongrang Zhang,et al.  Study on the ET Process of SOD at Cysteine Modified Gold Electrode , 1999 .

[49]  T. Ohsaka,et al.  Electrochemistry and electrocatalytic activities of superoxide dismutases at gold electrodes modified with a self-assembled monolayer. , 2004, Analytical chemistry.

[50]  T. Ohsaka,et al.  Mediated electron transfer of polyethylene oxide-modified superoxide dismutase by methyl viologen , 1995 .

[51]  A. Scheeline,et al.  Amperometric sensors for simultaneous superoxide and hydrogen peroxide detection. , 1997, Analytical chemistry.

[52]  K. Torimitsu,et al.  Continuous monitoring of L-glutamate released from cultured nerve cells by an online sensor coupled with micro-capillary sampling. , 1997, Biosensors & bioelectronics.