Surface-enhanced Raman scattering (SERS) spectroscopic analysis of herba houttuyniae decoction (HHD)

The normal Raman spectrum and surface-enhanced Raman scattering (SERS) spectrum of herba houttuyniae decoction (HHD) were tested and analyzed. The characteristic SERS bands of HHD were tentatively assigned. There was no Raman signal in normal Raman spectrum of HHD. However, as a result of the silver colloid enhanced effects on the Raman scattering of HHD, we observed that the SERS spectrum of HHD had primary thirteen SERS peaks such as 538, 620, 686, 730, 955, 1030, 1231, 1240, 1325, 1400, 1472, 1564 and 1651 cm-1, and there were six strong signals at 538, 620, 730, 955, 1325 and 1400 cm-1 bands. The results showed that the SERS spectroscopy might provide a new kind of high-sensitive, accurate, easy, and rapid detecting method for traditional Chinese medicine (TCM).

[1]  Bernhard Lendl,et al.  A New Method for Fast Preparation of Highly Surface-Enhanced Raman Scattering (SERS) Active Silver Colloids at Room Temperature by Reduction of Silver Nitrate with Hydroxylamine Hydrochloride , 2003 .

[2]  Gregory W Auner,et al.  Raman spectroscopic differentiation of activated versus non-activated T lymphocytes: an in vitro study of an acute allograft rejection model. , 2009, Journal of immunological methods.

[3]  S. Lane,et al.  Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. , 2006, Biophysical journal.

[4]  Rong Chen,et al.  Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering , 2011, International journal of nanomedicine.

[5]  Alexander M. Saletsky,et al.  Raman spectroscopy in comparative investigations of mechanisms of binding of three molecular probes – fluorescein, eosin, and erythrosin – to human serum albumin , 2008 .

[6]  Joseph Irudayaraj,et al.  Characterization of human breast epithelial cells by confocal Raman microspectroscopy. , 2006, Cancer detection and prevention.

[7]  Rong Chen,et al.  Surface-enhanced Raman spectroscopy of morphine in silver colloid , 2009 .

[8]  Rong Chen,et al.  Colorectal cancer detection by gold nanoparticle based surface-enhanced Raman spectroscopy of blood serum and statistical analysis. , 2011, Optics express.

[9]  J. Polak,et al.  In situ Characterisation of Living Cells by Raman Spectroscopy , 2002 .

[10]  Xue-LiangJiang,et al.  Different therapy for different types of ulcerative colitis in China , 2004 .

[11]  Wei Qian,et al.  Cancer cells assemble and align gold nanorods conjugated to antibodies to produce highly enhanced, sharp, and polarized surface Raman spectra: a potential cancer diagnostic marker. , 2007, Nano letters.

[12]  S. Lam,et al.  Near‐infrared Raman spectroscopy for optical diagnosis of lung cancer , 2003, International journal of cancer.

[13]  Dongsun Park,et al.  Anti-inflammatory effects of a Houttuynia cordata supercritical extract , 2010, Journal of veterinary science.

[14]  Rong Chen,et al.  Quick detection of traditional Chinese medicine ‘Atractylodis Macrocephalae Rhizoma’ pieces by surface-enhanced Raman spectroscopy , 2012 .

[15]  Harald Kneipp,et al.  Single Molecule Raman Scattering , 2006, Applied spectroscopy.

[16]  M. Wabuyele,et al.  Surface-enhanced Raman scattering for medical diagnostics and biological imaging , 2005 .

[17]  Zufang Huang,et al.  Nasopharyngeal cancer detection based on blood plasma surface-enhanced Raman spectroscopy and multivariate analysis. , 2010, Biosensors & bioelectronics.

[18]  W. Peticolas,et al.  Raman spectroscopy of DNA and proteins. , 1995, Methods in enzymology.