Biomedical applications of electrostatic layer-by-layer nano-assembly of polymers, enzymes, and nanoparticles

The introduction of electrostatic layer-by-layer (LbL) self-assembly has shown broad biomedical applications in thin film coating, micropatterning, nanobioreactors, artificial cells, and drug delivery systems. Multiple assembly polyelectrolytes and proteins are based on electrostatic interaction between oppositely charged layers. The film architecture is precisely designed and can be controlled to 1-nm precision with a range from 5 to 1000 nm. Thin films can be deposited on any surface including many widely used biomaterials. Microencapsulation of micro/nanotemplates with multilayers enabled cell surface modification, controlled drug release, hollow shell formation, and nanobioreactors. Both in vitro and in vivo studies indicate potential applications in biology, pharmaceutics, medicine, and other biomedical areas.

[1]  David K. Mills,et al.  Coating and selective deposition of nanofilm on silicone rubber for cell adhesion and growth , 2007, Cell Biochemistry and Biophysics.

[2]  N. Moroz,et al.  Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: factors regulating the protein release. , 2001, Biotechnology and bioengineering.

[3]  Y. Lvov,et al.  Nano-encapsulation of furosemide microcrystals for controlled drug release. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[4]  Andreas Voigt,et al.  pH-controlled macromolecule encapsulation in and release from polyelectrolyte multilayer nanocapsules. , 2001 .

[5]  Christopher J. Barrett,et al.  Fabrication of Microporous Thin Films from Polyelectrolyte Multilayers , 2000 .

[6]  J. Rapp,et al.  Protein-lipid interaction on the surface of a hydrophilic contact lens in vitro. , 1997, Current eye research.

[7]  Katsuhiko Ariga,et al.  ASSEMBLY OF MULTICOMPONENT PROTEIN FILMS BY MEANS OF ELECTROSTATIC LAYER-BY-LAYER ADSORPTION , 1995 .

[8]  R. Townsend.  Mycoplasma-like Organisms from Plants with ‘Yellows’ Diseases Lack a Spiroplasma-specific Antigen , 1983 .

[9]  R. Langer,et al.  Drug delivery and targeting. , 1998, Nature.

[10]  T. Desai,et al.  Fabrication of microtextured membranes for cardiac myocyte attachment and orientation. , 2000, Journal of biomedical materials research.

[11]  H. Möhwald,et al.  Polyelectrolyte multilayer capsule permeability control , 2002 .

[12]  J. Loscalzo,et al.  The potent platelet inhibitory effects of S-nitrosated albumin coating of artificial surfaces. , 1999, Journal of the American College of Cardiology.

[13]  C. Binz,et al.  Substantial reduction of platelet adhesion by heparin-coated stents. , 2001, Journal of interventional cardiology.

[14]  H. Möhwald,et al.  Assembly of polyelectrolyte multilayer films by consecutively alternating adsorption of polynucleotides and polycations , 1996 .

[15]  Kevin M. Chen,et al.  Selective Self-Organization of Colloids on Patterned Polyelectrolyte Templates , 2000 .

[16]  A. Voigt,et al.  Biological cells as templates for hollow microcapsules. , 2001, Journal of microencapsulation.

[17]  H. Möhwald,et al.  A realistic diffusion model for ultrathin polyelectrolyte films , 1996 .

[18]  A. R. Bausch,et al.  Colloidosomes: Selectively Permeable Capsules Composed of Colloidal Particles , 2002, Science.

[19]  Patrick S. Grant,et al.  Magnetic bio/nanoreactor with multilayer shells of glucose oxidase and inorganic nanoparticles , 2002 .

[20]  R. Arshady.  Preparation of biodegradable microspheres and microcapsules: 2. Polyactides and related polyesters , 1991 .

[21]  Caruso,et al.  Nanoengineering of inorganic and hybrid hollow spheres by colloidal templating , 1998, Science.

[22]  A. Ulman,et al.  Langmuir-Blodgett films , 1991 .

[23]  G. Sukhorukov,et al.  Multilayer alginate/protamine microsized capsules: encapsulation of alpha-chymotrypsin and controlled release study. , 2002, International journal of pharmaceutics.

[24]  Helmuth Möhwald,et al.  Novel Hollow Polymer Shells by Colloid-Templated Assembly of Polyelectrolytes. , 1998, Angewandte Chemie.

[25]  Jeffrey A. Hubbell,et al.  Thin Polymer Layers Formed by Polyelectrolyte Multilayer Techniques on Biological Surfaces , 1999 .

[26]  J. Feijen,et al.  Adhesion of coagulase-negative staphylococci to biomaterials. , 1983, Journal of general microbiology.

[27]  R. Friedman,et al.  The prevention of prosthetic infection using a cross-linked albumin coating in a rabbit model. , 1997, The Journal of bone and joint surgery. British volume.

[28]  Johannes Schmitt,et al.  Internal structure of layer-by-layer adsorbed polyelectrolyte films : a neutron and X-ray reflectivity study , 1993 .

[29]  G. Sukhorukov.  Designed nano-engineered polymer films on colloidal particles and capsules. , 2001 .

[30]  B. Tighe,et al.  Influence of Contact Lens Material Surface Characteristics and Replacement Frequency on Protein and Lipid Deposition , 1998, Optometry and vision science : official publication of the American Academy of Optometry.

[31]  Feng Hua,et al.  Lithographic approach to pattern self-assembled nanoparticle multilayers , 2002 .

[32]  J Hartmann,et al.  Microencapsulation by means of step-wise adsorption of polyelectrolytes. , 2000, Journal of microencapsulation.

[33]  B. Clement,et al.  Cell-based therapy of acute liver failure: The extracorporeal bioartificial liver , 1996, Cell Biology and Toxicology.

[34]  K. Oldhafer,et al.  A novel bioreactor design for in vitro reconstruction of in vivo liver characteristics. , 1995, Artificial organs.

[35]  K. Robinson,et al.  Reduced thrombus formation by hyaluronic acid coating of endovascular devices. , 2000, Arteriosclerosis, thrombosis, and vascular biology.

[36]  Rod J. Rohrich,et al.  Lipid Infiltration as a Possible Biologic Cause of Silicone Gel Breast Implant Aging , 1998, Plastic and reconstructive surgery.

[37]  Gero Decher,et al.  Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites , 1997 .

[38]  Daniel I. C. Wang,et al.  Engineering cell shape and function. , 1994, Science.

[39]  Bruck Sd,et al.  New ideas in biomaterials science--a path to engineered biomaterials. , 1994 .

[40]  Gleb B. Sukhorukov,et al.  Urease encapsulation in nanoorganized microshells. , 2001 .

[41]  Y. An,et al.  The Prevention of Prosthetic Infection Using a Cross-Linked Albumin in a Rabbit Model , 1997 .

[42]  Gero Decher,et al.  Toward Layered Polymeric Multicomposites , 1997 .

[43]  Franco Cacialli,et al.  Molecular-scale interface engineering for polymer light-emitting diodes , 2000, Nature.

[44]  G. Sukhorukov,et al.  Assembly of thin films by means of successive deposition of alternate layers of DNA and poly(allylamine) , 1993 .

[45]  Robert Langer,et al.  Moving smaller in drug discovery and delivery , 2002, Nature Reviews Drug Discovery.

[46]  K. Ariga,et al.  Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer‐by‐layer alternate adsorption , 1996, Biotechnology and bioengineering.

[47]  T. J. McCarthy,et al.  Poly(4-methyl-1-pentene)-Supported Polyelectrolyte Multilayer Films: Preparation and Gas Permeability1 , 1997 .

[48]  Katsuhiko Ariga,et al.  Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter , 1996 .

[49]  D E Wurster,et al.  Effect of Process Variables on the Microencapsulation of Vitamin A Palmitate by Gelatin-Acacia Coacervation , 2001, Drug development and industrial pharmacy.

[50]  H. Möhwald,et al.  Entrapment of alpha-chymotrypsin into hollow polyelectrolyte microcapsules. , 2001 .

[51]  R. Jalil,et al.  Biodegradable poly(lactic acid) and poly(lactide-co-glycolide) microcapsules: problems associated with preparative techniques and release properties. , 1990, Journal of microencapsulation.

[52]  Alain M. Jonas,et al.  Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties , 2000 .

[53]  F. Caruso,et al.  2. Assembly of Alternating Polyelectrolyte and Protein Multilayer Films for Immunosensing , 1997 .

[54]  Stephen W. Howell,et al.  Multiprotein Immunoassay Arrays Fabricated by Microcontact Printing , 2002 .

[55]  M. Reed,et al.  Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells. , 2002, Biosensors & bioelectronics.

[56]  M. Rubner,et al.  Molecular self-assembly of conducting polymers , 1994 .

[57]  G B Sukhorukov,et al.  Protein architecture: assembly of ordered films by means of alternated adsorption of oppositely charged macromolecules. , 1997, Membrane & cell biology.

[58]  D. Hammer,et al.  Polymersomes: tough vesicles made from diblock copolymers. , 1999, Science.

[59]  J. V. Alstine,et al.  Protein-rejecting ability of surface-bound dextran in end-on and side-on configurations: comparison to PEG. , 1995, Journal of biomedical materials research.

[60]  Shuvo Roy,et al.  Growth of connective tissue progenitor cells on microtextured polydimethylsiloxane surfaces. , 2002, Journal of biomedical materials research.

[61]  Jia-cong Shen,et al.  Immobilized bilayer glucose isomerase in porous trimethylamine polystyrene based on molecular deposition , 1994 .

[62]  H. Möhwald,et al.  Controlled Permeability of Polyelectrolyte Capsules via Defined Annealing , 2002 .

[63]  G. S. Ferguson,et al.  Stepwise Formation of Multilayered Nanostructural Films from Macromolecular Precursors , 1994, Science.

[64]  F Moussy,et al.  Characterization and biocompatibility studies of novel humic acids based films as membrane material for an implantable glucose sensor. , 2001, Biomacromolecules.

[65]  Wei Chen,et al.  Layer-by-layer deposition : A tool for polymer surface modification , 1997 .

[66]  L. Cima,et al.  Polymer substrates for controlled biological interactions , 1994, Journal of cellular biochemistry.

[67]  F. Caruso,et al.  Enzyme encapsulation in layer-by-layer engineered polymer multilayer capsules. , 2000 .

[68]  Johannes Schmitt,et al.  Proof of multilayer structural organization in self-assembled polycation-polyanion molecular films , 1994 .

[69]  Jayant Kumar,et al.  Oriented bacteriorhodopsin/polycation multilayers by electrostatic layer-by-layer assembly , 1998 .

[70]  A. Wan,et al.  Multi-layered microcapsules for cell encapsulation. , 2002, Biomaterials.

[71]  M. Gao,et al.  White‐light electroluminescence from self‐assembled Q‐CdSe/PPV multilayer structures , 1997 .

[72]  H. Möhwald,et al.  Hollow polyelectrolyte shells: Exclusion of polymers and Donnan equilibrium. , 1999 .

[73]  Silicone-induced foreign-body reaction after temporomandibular joint arthroplasty. Case report. , 1989, Australian dental journal.

[74]  R. Carmen,et al.  Lipid absorption by silicone heart valve poppets—in‐vivo and in‐vitro results , 1972 .

[75]  B D Ratner,et al.  Surface modification of polymers: chemical, biological and surface analytical challenges. , 1995, Biosensors & bioelectronics.

[76]  D. Mills,et al.  Coating bio-nanofilm on PDMS through layer-by-layer self-assembly , 2002, Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology.

[77]  H. Möhwald,et al.  Sustained release properties of polyelectrolyte multilayer capsules. , 2001 .

[78]  Allon I Hochbaum,et al.  Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films. , 2003, Biomacromolecules.

[79]  James F. Rusling,et al.  Direct Electrochemistry of Cofactor Redox Sites in a Bacterial Photosynthetic Reaction Center Protein , 1998 .

[80]  Ming Fang,et al.  Electrostatic layer-by-layer nanoassembly on biological microtemplates: platelets. , 2002, Biomacromolecules.

[81]  K. Ariga,et al.  Ultrathin films of charged polysaccharides assembled alternately with linear polyions. , 1998, Journal of biomaterials science. Polymer edition.

[82]  T. Kunitake,et al.  Formation of Ultrathin Polymer Layers on Solid Substrates by Means of Polymerization-Induced Epitaxy and Alternate Adsorption , 1996 .

[83]  J. Carrozza,et al.  Thrombotic and hemorrhagic complications of stenting coronary arteries: Incidence, management and prevention , 2005, Journal of Thrombosis and Thrombolysis.

[84]  H. Frank,et al.  Fast reversible electron transfer for photosynthetic reaction center from wild type Rhodobacter sphaeroides re-constituted in polycation sandwiched monolayer film. , 1999, Bioelectrochemistry and bioenergetics.

[85]  K. Ariga,et al.  Layer-by-Layer Assembly of Alternate Protein/Polyion Ultrathin Films. , 1994 .

[86]  Michael F. Rubner,et al.  Controlling Bilayer Composition and Surface Wettability of Sequentially Adsorbed Multilayers of Weak Polyelectrolytes , 1998 .

[87]  T. Chang,et al.  Hepatocytes immobilised by microencapsulation in artificial cells: effects on hyperbilirubinemia in Gunn rats. , 1989, Biomaterials, artificial cells, and artificial organs.

[88]  Helmuth Möhwald,et al.  Studies on the drug release properties of polysaccharide multilayers encapsulated ibuprofen microparticles. , 2001 .

[89]  R. Carmen,et al.  Lipid absorption by silicone rubber heart valve poppets--in-vivo and in-vitro results. , 1972, Journal of biomedical materials research.

[90]  J. Schlenoff,et al.  Sprayed Polyelectrolyte Multilayers , 2000 .

[91]  H. Möhwald,et al.  Lipid Coating on Polyelectrolyte Surface Modified Colloidal Particles and Polyelectrolyte Capsules , 2000 .

[92]  F. Caruso,et al.  Enzyme Multilayers on Colloid Particles: Assembly, Stability, and Enzymatic Activity , 2000 .

[93]  H. Ringsdorf,et al.  Interactions, Structure, and Stability of Photoreactive Bolaform Amphiphile Multilayers , 1995 .

[94]  Helmuth Möhwald,et al.  Assembly, structural characterization, and thermal behavior of layer-by-layer deposited ultrathin films of poly(vinyl sulfate) and poly(allylamine) , 1993 .

[95]  Johannes Schmitt,et al.  New nanocomposite films for biosensors: layer-by-layer adsorbed films of polyelectrolytes, proteins or DNA , 1993 .

[96]  A. Ulman,et al.  Ultrathin organic films: From Langmuir-Blodgett to self assembly , 1991 .

[97]  John W. Park,et al.  Immunoliposomes for cancer treatment. , 1997, Advances in pharmacology.

[98]  Paula T. Hammond,et al.  Two component particle arrays on patterned polyelectrolyte multilayer templates , 2002 .

[99]  M. Fang,et al.  Applications of the electrostatic layer-by-layer self-assembly technique in biomedical engineering , 2002, Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology.

[100]  Y. An,et al.  Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. , 1998, Journal of biomedical materials research.

[101]  George M. Whitesides,et al.  Self-Organization of Organic Liquids on Patterned Self-Assembled Monolayers of Alkanethiolates on Gold , 1994 .

[102]  K. Ariga,et al.  Activity and stability of glucose oxidase in molecular films assembled alternately with polyions. , 1999, Journal of bioscience and bioengineering.

[103]  F. Caruso,et al.  Biocolloids with ordered urease multilayer shells as enzymatic reactors. , 2001, Analytical chemistry.

[104]  M. Akashi,et al.  Alternating bioactivity of polymeric layer-by-layer assemblies: anticoagulation vs procoagulation of human blood. , 2002, Biomacromolecules.