Nanostructured polymer/nanophase ceramic composites enhance osteoblast and chondrocyte adhesion.

Osteoblast (bone-forming cell) and chondrocyte (cartilage-synthesizing cell) adhesion on novel nanostructured polylactic/glycolic acid (PLGA) and titania composites were investigated in the present in vitro study. Nanostructured polymers were created by chemically treating micron-structured PLGA with select concentrations of NaOH for various periods of time. Dimensions of ceramics were controlled by utilizing either micron or nanometer grain size titania. Compared with surfaces with conventional or micron surface roughness dimensions, results provided the first evidence of increased osteoblast and chondrocyte adhesion on 100 wt% PLGA films with nanometer polymer surface roughness dimensions. Results also confirmed other literature reports of enhanced osteoblast adhesion on 100 wt% nanometer compared with conventional grain size titania compacts; however, the present study provided the first evidence that decreasing titania grain size into the nanometer range did not influence chondrocyte adhesion. Finally, osteoblast and chondrocyte adhesion increased on 70/30 wt% PLGA/titania composites formulated to possess nanosurface rather than conventional surface feature dimensions. The present study, thus, provided evidence that these nanostructured PLGA/titania composites may possess the ability to simulate surface and/or chemical properties of bone and cartilage, respectively, to allow for exciting alternatives in the design of prostheses with greater efficacy.

[1]  T. Webster,et al.  Enhanced functions of osteoblasts on nanophase ceramics. , 2000, Biomaterials.

[2]  Richard W. Siegel,et al.  Design and evaluation of nanophase alumina for orthopaedic/dental applications , 1999 .

[3]  H. J. Mankin,et al.  Instructional Course Lectures, The American Academy of Orthopaedic Surgeons - Articular Cartilage. Part II: Degeneration and Osteoarthrosis, Repair, Regeneration, and Transplantation*† , 1997 .

[4]  N. Forest,et al.  Ultrastructural Study of Bone Formation on Synthetic Hydroxyapatite in Osteoblast Cultures , 1991 .

[5]  R Langer,et al.  Surface hydrolysis of poly(glycolic acid) meshes increases the seeding density of vascular smooth muscle cells. , 1998, Journal of biomedical materials research.

[6]  D. Guidolin,et al.  Morphological analysis of articular cartilage biopsies from a randomized, clinical study comparing the effects of 500-730 kDa sodium hyaluronate (Hyalgan) and methylprednisolone acetate on primary osteoarthritis of the knee. , 2001, Osteoarthritis and cartilage.

[7]  T. Webster,et al.  Mechanisms of enhanced osteoblast adhesion on nanophase alumina involve vitronectin. , 2001, Tissue engineering.

[8]  J. Suh,et al.  Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. , 2000, Biomaterials.

[9]  H. M. Kim,et al.  Graded surface structure of bioactive titanium prepared by chemical treatment. , 1999, Journal of biomedical materials research.

[10]  S. Ayad.  The extracellular matrix factsbook , 1998 .

[11]  T. Aigner,et al.  Transplantation of allograft chondrocytes embedded in agarose gel into cartilage defects of rabbits. , 1998, Osteoarthritis and cartilage.

[12]  K. Athanasiou,et al.  Ex vivo synthesis of articular cartilage. , 2000, Biomaterials.

[13]  S. Stea,et al.  Bone demineralization induced by cementless alumina-coated femoral stems. , 1994, The Journal of arthroplasty.

[14]  T. Webster,et al.  Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics. , 2000, Journal of biomedical materials research.

[15]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[16]  Robert Langer,et al.  Preparation and characterization of poly(l-lactic acid) foams , 1994 .

[17]  O. Böstman,et al.  Response of articular cartilage and subchondral bone to internal fixation devices made of poly-L-lactide: a histomorphometric and microradiographic study on rabbits. , 2000, Biomaterials.

[18]  C. Loty,et al.  Bioactive glass-ceramic containing crystalline apatite and wollastonite initiates biomineralization in bone cell cultures , 1994, Calcified Tissue International.

[19]  J. Hollinger,et al.  Bone Regeneration Materials for the Mandibular and Craniofacial Complex , 1992 .

[20]  T. Webster,et al.  Enhanced osteoclast-like cell functions on nanophase ceramics. , 2001, Biomaterials.

[21]  H. Yoshikawa,et al.  Alumina ceramic prostheses for bone tumor surgery , 2004, Archives of Orthopaedic and Trauma Surgery.

[22]  T. Matsuda,et al.  The in vitro response of osteoblasts to bioactive glass. , 1987, Biomaterials.

[23]  R Langer,et al.  In vitro generation of osteochondral composites. , 2000, Biomaterials.

[24]  J. Davies,et al.  Extracellular matrix production by osteoblasts on bioactive substrata in vitro. , 1988, Scanning microscopy.

[25]  J. B. Brunski,et al.  36. Influence of Biomechanical Factors at the Bone-Biomaterial Interface , 1991 .

[26]  T. Webster,et al.  Osteoblast adhesion on nanophase ceramics. , 1999, Biomaterials.