We would like to thank all staff at IIID for their assistance and input. Introduction HLA typing • Required prior to organ and stem cell transplantation. • Links to several infectious and autoimmune diseases. • Commonly done using sequence based typing. Challenge • In SBT typing, replace Sanger sequencing with FLX 454 sequencing. • To type HLA antigens with less ambiguous results using a high throughput workflow in a cost effective and time efficient manner. Current study • Uses 454 GS FLX sequencing for typing HLA. • HLA antigens typed based on exon2 & 3 for class I (A, B and C loci) and exon 2 for class II (DRB1 locus). • Multiplex identifier (MID) tags added during PCR amplification to allow sample pooling in sequencing.