An improved mRFP1 adds red to bimolecular fluorescence complementation

Protein-protein interactions are fundamental to virtually every aspect of cellular functions. Blue, green and yellow bimolecular fluorescence complementation (BiFC) systems based on GFP and its variants allow the investigation of protein-protein interactions in vivo. We have developed the first red BiFC system based on an improved monomeric red fluorescent protein (mRFP1-Q66T), expanding the range of possible applications for BiFC.

[1]  C. Riedel,et al.  Improved version of the red fluorescent protein (drFP583/DsRed/RFP). , 2002, BioTechniques.

[2]  Nathan C Shaner,et al.  A guide to choosing fluorescent proteins , 2005, Nature Methods.

[3]  S J Remington,et al.  Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[4]  B. Glick,et al.  Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed) , 2002, Nature Biotechnology.

[5]  Chang‐Deng Hu,et al.  Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. , 2002, Molecular cell.

[6]  Konstantin A Lukyanov,et al.  Common pathway for the red chromophore formation in fluorescent proteins and chromoproteins. , 2004, Chemistry & biology.

[7]  Chang‐Deng Hu,et al.  Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis , 2003, Nature Biotechnology.

[8]  Vladislav V Verkhusha,et al.  Conversion of the monomeric red fluorescent protein into a photoactivatable probe. , 2005, Chemistry & biology.

[9]  J. Uhrig,et al.  Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins. , 2004, The Plant journal : for cell and molecular biology.

[10]  R. Tsien,et al.  Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells , 2002, Science.

[11]  Frank Kirchhoff,et al.  Expression of reef coral fluorescent proteins in the central nervous system of transgenic mice , 2005, Molecular and Cellular Neuroscience.

[12]  R. Tsien,et al.  Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein , 2004, Nature Biotechnology.

[13]  R. Tsien,et al.  A monomeric red fluorescent protein , 2002, Proceedings of the National Academy of Sciences of the United States of America.