Electrochemical Behaviour of the Anticancer Dacarbazine-Cu2+ Complex and Its Analytical Applications

Electrochemical reduction of the dacarbazine-Cu2+ complex was investigated using cyclic voltammetry and square wave voltammetry at a hanging mercury drop electrode. The reduction of the dacarbazine-Cu2+ complex is irreversible. A reduction mechanism is proposed comprising a one-electron reduction of the Cu2+ directly within the complex. The sharp peak of the adsorbed dacarbazine-Cu2+ complex associated with an effective interfacial accumulation facilitates the determination of the anticancer drug dacarbazine in pharmaceutical formulations and biological fluids. Detection limits for dacarbazine of 6.12×10−10 M, 1.57×10−10 M and 1.97×10−9 M were achieved for the determination of the drug in vial, human urine and serum, respectively.

[1]  Yuhui Yang,et al.  Validated hydrophilic interaction LC-MS/MS method for simultaneous quantification of dacarbazine and 5-amino-4-imidazole-carboxamide in human plasma. , 2008, Talanta.

[2]  Y. Nakatsu,et al.  Modes of actions of two types of anti-neoplastic drugs, dacarbazine and ACNU, to induce apoptosis. , 2007, Carcinogenesis.

[3]  Min Song,et al.  Nano-titanium dioxide enhanced biosensing of the interaction of dacarbazine with DNA and DNA bases , 2006 .

[4]  J. Kirkwood,et al.  Re-evaluating the role of dacarbazine in metastatic melanoma: what have we learned in 30 years? , 2004, European journal of cancer.

[5]  N. Zhang,et al.  Voltammetric study of the interaction of ciprofloxacin-copper with nucleic acids and the determination of nucleic acids. , 2004, Talanta.

[6]  R. Stancanelli,et al.  Improvement in solubility and dissolution rate of flavonoids by complexation with beta-cyclodextrin. , 2004, Journal of pharmaceutical and biomedical analysis.

[7]  Nan Zhang,et al.  Voltammetric study of the interaction of lomefloxacin (LMF)–Mg(II) complex with DNA and its analytical application , 2003 .

[8]  Xuemei Wang,et al.  A SPECTROSCOPIC STUDY ON THE DNA BINDING BEHAVIOR OF THE ANTICANCER DRUG DACARBAZINE , 2002 .

[9]  Arnold G. Fogg,et al.  Cathodic stripping voltammetric determination of ceftazidime with reactive accumulation at a poly-l-lysine modified hanging mercury drop electrode , 1999 .

[10]  J. T. Stewart,et al.  ISOCRATIC DETERMINATION OF DACARBAZINE AND RELATED IMPURITIES 2-AZAHYPOXANTHINE AND 5-AMINO-IMIDAZOLE-4-CARBOXAMIDE BY HPLC ON AN AVIDIN PROTEIN COLUMN , 1999 .

[11]  W. Smyth,et al.  AN ELECTROANALYTICAL STUDY OF THE ANTICANCER DRUG DACARBAZINE , 1987 .

[12]  A. Jackson,et al.  Simultaneous determination of dacarbazine, its photolytic degradation product, 2-azahypoxanthine, and the metabolite 5-aminoimidazole-4-carboxamide in plasma and urine by high-pressure liquid chromatography , 1985, Antimicrobial Agents and Chemotherapy.

[13]  E. Golovinsky,et al.  Pharmacobiochemistry of arylalkyltriazenes and their application in cancer chemotherapy. , 1985, Pharmacology & therapeutics.

[14]  H. C. Freeman,et al.  The crystal structures of two copper(II) complexes of the antitumor agent 5‐(3,3‐dimethyl‐1‐triazenyl)imidazole‐4‐carboxamide , 1979 .

[15]  E. Laviron,et al.  Adsorption, autoinhibition and autocatalysis in polarography and in linear potential sweep voltammetry , 1974 .