The kinetics of protein salting‐Out: Precipitation of yeast enzymes by ammonium sulfate

Protein solubility can be adequately represented by the classical Cohn equation for the salting‐out of alcohol dehydrogenase and fumarase from clarified yeast homogenate with ammonium sulfate. However, the constant β in this equation is a function of the contacting procedure employed. The kinetics of continuous salting‐out were similar for alcohol dehydrogenase and fumarase. The overall rate equation for precipitation had a variable order which was high initially, up to 3.1, but approached unity on completion of precipitation. This was followed by a partial resolution stage which was first order with respect to the concentration driving force. Precipitate particle size was estimated as 0.5 to 5 μm with continuous flow precipitation producing the largest particles.

[1]  C. Hoogendoorn,et al.  Model studies on mixers in the viscous flow region , 1967 .

[2]  M. Tirrell,et al.  Shear modification of enzyme kinetics , 1975 .

[3]  F DiJeso,et al.  Ammonium sulfate concentration conversion nomograph for 0 degrees. , 1968 .

[4]  M. Moo‐Young,et al.  The blending efficiencies of some impellers in batch mixing , 1972 .

[5]  Daniel I. C. Wang,et al.  Microbial cell recovery enhancement through flocculation , 1970 .

[6]  W. L. Badger,et al.  Rate of Growth of Crystals in Aqueous Solution1,2 , 1927 .

[7]  M. Lilly,et al.  Release of enzymes from bakers' yeast by disruption in an industrial homogenizer , 1971, Biotechnology and bioengineering.

[8]  P Dunnill,et al.  Salting-out of enzymes with ammonium sulphate. , 1971, Biotechnology and bioengineering.

[9]  A. B. Metzner.  Agitation of non‐Newtonian fluids , 1957 .

[10]  A. Nienow,et al.  The mass transfer driving force for high mass flux , 1969 .

[11]  H. Bull,et al.  Water and solute binding by proteins. 1. Electrolytes. , 1970, Archives of biochemistry and biophysics.

[12]  S. Nagata,et al.  A Study on the Mixing of High-viscosity Liquid , 1957 .

[13]  M. Lilly,et al.  The clarification of mechanically disrupted yeast suspensions by rotary vacuum precoat riltration , 1973 .

[14]  J. Nývlt,et al.  Metastable zone-width of some aqueous solutions , 1970 .

[15]  E. Webb,et al.  Enzyme fractionation by salting-out: a theoretical note. , 1961, Advances in protein chemistry.

[16]  T. Bücher,et al.  Crystallized enzymes from the myogen of rabbit skeletal muscle. , 1960, Advances in protein chemistry.

[17]  P Dunnill,et al.  The precipitation of enzymes from cell extracts of Saccharomyces cerevisiae by polyethyleneglycol. , 1973, Biochimica et biophysica acta.

[18]  John Garside,et al.  The concept of effectiveness factors in crystal growth , 1971 .

[19]  S. Charm,et al.  Enzyme inactivation with shearing. , 1970, Biotechnology and bioengineering.

[20]  A. Nienow,et al.  Dissolution mass transfer in a turbine agitated baffled vessel , 1969 .

[21]  Edwin J. Cohn,et al.  THE PHYSICAL CHEMISTRY OF THE PROTEINS , 1925 .

[22]  C. F. Ferraro,et al.  Particle size measurements and properties of AVIAMIDE®-6 microcrystalline nylon , 1971 .