Efficiency and safety of human acellular dermal matrix for repair of leaking filtering blebs in rabbit eye

This study was aimed to investigate the efficiency and safety of human acellular dermal matrix (ADM) for the repair of leaking glaucoma filtering blebs in the rabbit eye model. A total of 48 New Zealand rabbits with leaking filtering blebs were randomized into ADM group, amniotic membrane group, and conjunctiva overlap group. The intraocular pressure (IOP) was measured preoperatively, 1 day, 1 week, 1 month, 3 months and 6 months post-operation. Slit lamp, anterior segment optical coherent tomography (AS-OCT) images, and histopathological changes were recorded 1 week, 1 month, 3 months, and 6 months post-operation. The IOP of the ADM group was lower than the control groups after the operation, but statistical significance was observed at 1 month post-operation (ANOVA F=13.006, P<0.001, LSD t-test P=0.001, <0.001). Slit lamp and AS-OCT images revealed transparent ADM patches, the filtering blebs up to 3 months post-operation, and the conjunctival neovascularization occurred within 3 months post-operation. However, the filtering bleb could only be maintained up to 1 month, and the conjunctival neovascularization occurred in 1 month post-operation with continual corneal neovascularization in the control groups. Histopathologically, the ADM patch was found up to 6 months post-operation. ADM might be effective and a safe biomaterial for repairing the leaking filtering blebs.

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