Characteristics of excitatory amino acid uptake in cultures from neurons and glia from the retina

3H‐D‐Aspartate uptake was biochemically characterized in cultures from chick retina enriched in glial (Müller) cells or neurons during progressive days in vitro (DIV). In the neuronal cultures a high‐affinity, Na+‐dependent system was found with Km = 8–13 μM and pharmacological characteristics in agreement with those of reuptake systems in other regions of the CNS. The uptake system in glial cells showed a lower affinity, with Km = 100–135 μM. In both cases, uptake wastemperature and energy dependent. A sharp increase in the Vmax of uptake was observed in both neuronal and glial cultures at 5 DIV, at which time morphologically mature synapses have been shown to be present in retinal cultures. A parallel increase in the pharmacological specificity of the uptake system in neuronal cultures was observed, with a rise in the efficiency of D‐Asp, L‐Asp, L‐Glu, and DL‐asp‐ β‐hydroxamate for inhibiting 3H‐D‐As‐partate uptake. Results suggest the possibility of re‐uptake participating in the regulation of extracellular glutamate concentration during development.

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