Hydrogen production by mixed bacteria through dark and photo fermentation

Abstract Mixed bacteria were used to improve hydrogen yield from cassava starch in combination of dark and photo fermentation. In dark fermentation, mixed anaerobic bacteria (mainly Clostridium species) were used to produce hydrogen from cassava starch. Substrate concentration, fermentation temperature and pH were optimized as 10.4 g/l, 31 °C and 6.3 by response surface methodology (RSM). The maximum hydrogen yield and production rate in dark fermentation were 351 ml H2/g starch (2.53 mol H2/mol hexose) and 334.8 ml H2/l/h, respectively. In photo fermentation, immobilized mixed photosynthetic bacteria (PSB, mainly Rhodopseudomonas palustris species) were used to produce hydrogen from soluble metabolite products (SMP, mainly acetate and butyrate) of dark fermentation. The maximum hydrogen yield in photo fermentation was 489 ml H2/g starch (3.54 mol H2/mol hexose). The total hydrogen yield was significantly increased from 402 to 840 ml H2/g starch (from 2.91 to 6.07 mol H2/mol hexose) by mixed bacteria and cell immobilization in combination of dark and photo fermentation.

[1]  H. Álvarez,et al.  Dynamic models for the production of glucose syrups from cassava starch , 2008 .

[2]  Tong Zhang,et al.  Biohydrogen production from starch in wastewater under thermophilic condition. , 2003, Journal of environmental management.

[3]  T. Noike,et al.  Effects of temperature and pH on the growth of heterotrophic bacteria in waste stabilization ponds , 1996 .

[4]  D. L. Hawkes,et al.  Sustainable fermentative hydrogen production: challenges for process optimisation , 2002 .

[5]  H. Argun,et al.  Effects of light source, intensity and lighting regime on bio-hydrogen production from ground wheat starch by combined dark and photo-fermentations , 2010 .

[6]  Jo‐Shu Chang,et al.  Feasibility study on fermentative conversion of raw and hydrolyzed starch to hydrogen using anaerobic mixed microflora , 2007 .

[7]  Jo-Shu Chang,et al.  Exploring optimal environmental factors for fermentative hydrogen production from starch using mixed anaerobic microflora , 2008 .

[8]  Jun Cheng,et al.  Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE. , 2008, Journal of environmental sciences.

[9]  T. Noike,et al.  Characteristics of anaerobic ammonia removal by a mixed culture of hydrogen producing photosynthetic bacteria. , 2004, Bioresource technology.

[10]  Jo‐Shu Chang,et al.  HRT-dependent hydrogen production and bacterial community structure of mixed anaerobic microflora in suspended, granular and immobilized sludge systems using glucose as the carbon substrate , 2008 .

[11]  Kefa Cen,et al.  Combination of dark- and photo-fermentation to enhance hydrogen production and energy conversion efficiency , 2009 .

[12]  Kefa Cen,et al.  Production of hydrogen and methane from potatoes by two-phase anaerobic fermentation. , 2008, Bioresource technology.

[13]  Michael Cooney,et al.  Two-phase anaerobic digestion for production of hydrogen-methane mixtures. , 2007, Bioresource technology.

[14]  Chiu-Yue Lin,et al.  Microbial community structure of a starch-feeding fermentative hydrogen production reactor operated under different incubation conditions , 2008 .

[15]  Jo-Shu Chang,et al.  Batch and continuous biohydrogen production from starch hydrolysate by Clostridium species , 2008 .

[16]  Chiu-Yue Lin,et al.  Fermentative hydrogen production from starch using natural mixed cultures , 2008 .

[17]  Jie Ding,et al.  Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology. , 2009, Bioresource technology.

[18]  Jianquan Shen,et al.  Effects of culture and medium conditions on hydrogen production from starch using anaerobic bacteria. , 2004, Journal of bioscience and bioengineering.

[19]  H. Argun,et al.  Batch dark fermentation of powdered wheat starch to hydrogen gas : Effects of the initial substrate and biomass concentrations , 2008 .

[20]  H Yokoi,et al.  Microbial hydrogen production from sweet potato starch residue. , 2001, Journal of bioscience and bioengineering.

[21]  Peng Zhang,et al.  Efficient hydrogen gas production from cassava and food waste by a two-step process of dark fermentation and photo-fermentation , 2009 .

[22]  Han-Qing Yu,et al.  Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures , 2002 .

[23]  Jo‐Shu Chang,et al.  Combining enzymatic hydrolysis and dark–photo fermentation processes for hydrogen production from starch feedstock: A feasibility study , 2008 .

[24]  Jie Ding,et al.  Simultaneous biohydrogen production and starch wastewater treatment in an acidogenic expanded granular sludge bed reactor by mixed culture for long-term operation , 2008 .

[25]  Jun Cheng,et al.  Improving hydrogen production from cassava starch by combination of dark and photo fermentation , 2009 .

[26]  Jianquan Shen,et al.  Effect of temperature and iron concentration on the growth and hydrogen production of mixed bacteria , 2006 .