Extraction of curcuminoids from Curcuma longa: comparative study between batch extraction and novel three phase partitioning

Abstract Curcuminoids, the active components of dried rhizome of Curcuma longa have been extracted using batch extraction and three-phase partitioning (TPP) process. The effect of different processing parameters, namely different solvents, extraction time, ammonium sulfate concentration, slurry to tert-butanol ratio, and solute to aqueous ratio on extraction efficiency of TPP, was studied to attain maximum extraction yield. The highest yield of 58.38 mg/g was achieved at 40±2 °C in 150 min, with saturated ammonium sulfate 30% (w/v), slurry to tert-butanol ratio 1:1 (v/v), and turmeric powder to water ratio 1:40 (w/v) in TPP. However, batch extraction using ethanol as a solvent yielded 52.77 mg/g in 180 min extraction time at 40±2 °C with 1:40 (w/v) turmeric powder to water ratio and 400 rpm agitation speed. In view of reference method, i.e., Soxhlet extraction (100%), TPP showed 65.63% yield in 150 min and batch exhibited 59.92% in 180 min. The turmeric extracts obtained by different methods exhibited excellent antioxidant and anti-inflammatory activities equivalent to their respective reference standards. Hence, TPP extraction process assures a rapid and improved recovery of curcuminoids with excellent therapeutic properties. Graphical Abstract Curcuminoids, the active components of dried rhizome of Curcuma longa has been extracted using batch extraction and three-phase partitioning (TPP) process. Research highlights Batch and three phase partitioning (TPP) methods were successfully studied and compared for curcuminoids extraction. Influence of different processing parameters on TPP and batch were optimized. Three phase partition gives more yield than the batch extraction process. The extracts obtained showed excellent antioxidant and anti-inflammatory properties. TPP was found to be rapid method of extraction compared to batch process.

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