Sodium dodecyl sulfate-glycerol polyacrylamide slab gel electrophoresis for the resolution of apolipoprot eins

We describe the resolution of the plasma apolipoproteins with molecular weights from 8,800 to greater than 550,000, using a 3.5% sodium dodecyl sulfate-glycerol polyacrylamide slab gel system. The simplicity of this system and the resolution of proteins over a broad range of molecular weights will make it particularly useful in investigations of apolipoprotein composition of plasma lipoproteins.-Maguire, G. F., M. Lee, and P. W. Connelly. Sodium dodecyl sulfate-glycerol polyacrylamide slab gel electrophoresis for the resolution of apolipoproteins. J. Lipid a s . 1989. 3 0 757-761.

[1]  R. Gregg,et al.  Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. , 1986, Journal of lipid research.

[2]  R. Mahley,et al.  Plasma lipoproteins: apolipoprotein structure and function. , 1984, Journal of lipid research.

[3]  H. Brewer,et al.  Two-dimensional electrophoresis of human plasma apolipoproteins. , 1984, Clinical chemistry.

[4]  A. Kuksis,et al.  SDS-glycerol polyacrylamide gel electrophoresis of plasma apolipoproteins. , 1982, Biochimica et biophysica acta.

[5]  J. Kane,et al.  Heterogeneity of apolipoprotein B: isolation of a new species from human chylomicrons. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[6]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[7]  N. Tolbert,et al.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. , 1978, Analytical biochemistry.

[8]  K. Kuehl,et al.  Separation of apolipoproteins by an acrylamide-gradient sodium dodecyl sulfate gel electrophoresis system. , 1976, Biochimica et biophysica acta.

[9]  A. Scanu,et al.  Solubility in aqueous solutions of ethanol of the small molecular weight peptides of the serum very low density and high density lipoproteins: relevance to the recovery problem during delipidation of serum lipoproteins. , 1971, Analytical biochemistry.

[10]  R. Havel,et al.  The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. , 1955, The Journal of clinical investigation.

[11]  J. C. Osborne.  Delipidation of plasma lipoproteins. , 1986, Methods in enzymology.