Identification of the Species of Origin of Residual Blood on Lithic Material

The examination of stone-tool edges for blood-antigen residue is a relatively new technique in the archaeological analysis of lithic material. To date, a number of different methods have been employed to determine the species of origin of residual blood, such as Ouchterlony double-diffusion (Ouchterlony 1968) and radioimmunoassays (RIAs) (Lowenstein 1985, 1986). These techniques have been of limited use due to problems of sensitivity, cost, and applicability to archaeological field conditions. Enzyme immunoassays (EIAs) utilizing a nitrocellulose protein-binding membrane have shown that it is possible to retain both specificity and sensitivity in a technique that can endure the rigors of archaeological field work and the vicissitudes of differential preservation (Tersak and Hyland 1988). However, due to cross-reactivity, species identification of blood residue using immunological techniques can be problematic when it is necessary to distinguish individual species within a single genus or family. Recent work has shown that careful refinement of antisera can eliminate cross-reactivity, thereby increasing specificity (Berkeley Antibody Company, personal communication 1987). This refinement is accomplished by absorbing any antibodies from the antisera that may cross-react with antigenic sites held in common by closely related species. The ramifications of the successful implementation of this technique are discussed in terms of Paleoindian artifact function as well as paleoeconomic, paleoenvironmental, and paleodietary reconstruction using a case study from the Shoop site in central Pennsylvania.

[1]  G. Harvey.  “A Material World” National Museum of American History, Smithsonian Institution, Fourteenth and Constitution Ave. NW, Washington, DC 20560 , 1991 .

[2]  U. Franklin,et al.  A study of the feasibility of detecting blood residue on artifacts , 1988 .

[3]  T. Loy,et al.  Potential applications of the organic residues on ancient tools , 1986 .

[4]  J. Southon,et al.  Radiocarbon Dating Blood Residues on Prehistoric Stone Tools , 1986, Radiocarbon.

[5]  J. Lowenstein.  Molecular Approaches to the Identification of Species , 1985 .

[6]  M. Steward.  Monoclonal Antibodies and Cancer , 1985 .

[7]  P. Tijssen,et al.  Practice and theory of enzyme immunoassays , 1985 .

[8]  D. W. Oates,et al.  Field Technique for the Identification of Deer Blood , 1983 .

[9]  T. Loy.  Prehistoric Blood Residues: Detection on Tool Surfaces and Identification of Species of Origin , 1983, Science.

[10]  S. Washburn,et al.  Piltdown jaw confirmed as orang , 1982, Nature.

[11]  A. J. Sinclair,et al.  Identification of meat according to species by isoelectric focusing. , 1982, Australian veterinary journal.

[12]  M. Allison,et al.  ABO blood groups in Chilean and Peruvian mummies. II. Results of agglutination-inhibition technique. , 1978, American journal of physical anthropology.

[13]  R. Barraco.  Preservation of proteins in mummified tissues. , 1978, American journal of physical anthropology.

[14]  R. Washino.  Identification of Host Blood Meals in Arthropods. , 1977 .

[15]  J. Sabloff,et al.  A history of American archaeology , 1974 .

[16]  G. Sensabaugh,et al.  Protein stability in preserved biological remains , 1971 .

[17]  A. Wilson,et al.  Protein stability in preserved biological remains: II. Modification and aggregation of proteins in an 8-year-old sample of dried blood , 1971 .