2 Cl-- cotransporter : A Novel Marker for Identifying Freshwater-and Seawater-type Mitochondria-rich Cells in Gills of the Euryhaline Tilapia , Oreochromis mossambicus

Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K+,2Cl--cotransporter: a novel marker for identifying freshwaterand seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+,2Cl--cotransporter (NKCC) were examined in freshwaterand seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwateror seawater-type MR cells in euryhaline tilapia. http://www.sinica.edu.tw/zool/zoolstud/42.1/186.pdf

[1]  P. Hwang,et al.  Mitochondria-rich cells in the branchial epithelium of the teleost,Oreochromis mossambicus, acclimated to various hypotonic environments , 1996, Fish Physiology and Biochemistry.

[2]  P. Hwang,et al.  The relationship between `deep-hole' mitochondria-rich cells and salinity adaptation in the euryhaline teleost, Oreochromis mossambicus , 2000, Fish Physiology and Biochemistry.

[3]  P. Hwang,et al.  Expression of mRNA and protein of Na+-K+-ATPase α subunit in gills of tilapia (Oreochromis mossambicus) , 1998, Fish Physiology and Biochemistry.

[4]  G. Flik,et al.  Sodium dependent ion transporters in trout gills , 1997, Fish Physiology and Biochemistry.

[5]  K. Spring,et al.  Chloride transport activation by plasma osmolarity during rapid adaptation to high salinity of Fundulus heteroclitus , 1995, The Journal of Membrane Biology.

[6]  D. Kültz,et al.  Cellular and epithelial adjustments to altered salinity in the gill and opercular epithelium of a cichlid fish (Oreochromis mossambicus) , 2004, Cell and Tissue Research.

[7]  W. Marshall,et al.  Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water. , 2002, The Journal of experimental biology.

[8]  S. McCormick,et al.  Cotransporter Abundance and Location in Atlantic Salmon: Effects of Seawater and Smolting , 2001 .

[9]  Tsung-Han Lee,et al.  Morphology and Function of Gill Mitochondria‐Rich Cells in Fish Acclimated to Different Environments , 2001, Physiological and Biochemical Zoology.

[10]  G. Flik,et al.  Cortisol increases Na(+)/K(+)-ATPase density in plasma membranes of gill chloride cells in the freshwater tilapia Oreochromis mossambicus. , 2000, The Journal of experimental biology.

[11]  J. M. Wilson,et al.  Immunolocalization of ion-transport proteins to branchial epithelium mitochondria-rich cells in the mudskipper (Periophthalmodon schlosseri). , 2000, The Journal of experimental biology.

[12]  K. Uchida,et al.  Excellent Salinity Tolerance of Mozambique Tilapia (Oreochromis mossambicus): Elevated Chloride Cell Activity in the Branchial and Opercular Epithelia of the Fish Adapted to Concentrated Seawater , 2000 .

[13]  P. Piermarini,et al.  Ionic transport in the fish gill epithelium , 1999 .

[14]  Ming-Jiun Yu,et al.  Isoform expression of Na+-K+-ATPase α-subunit in gills of the teleost Oreochromis mossambicus. , 1998, American journal of physiology. Regulatory, integrative and comparative physiology.

[15]  P. Hwang,et al.  Immune localization of prolactin receptor in the mitochondria‐rich cells of the euryhaline teleost (Oreochromis mossambicus) gill , 1997, FEBS letters.

[16]  C. Lytle,et al.  Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies. , 1995, The American journal of physiology.

[17]  J. A. Payne,et al.  Primary Structure, Functional Expression, and Chromosomal Localization of the Bumetanide-sensitive Na-K-Cl Cotransporter in Human Colon (*) , 1995, The Journal of Biological Chemistry.

[18]  J. A. Payne,et al.  Molecular characterization of the epithelial Na-K-Cl cotransporter isoforms. , 1995, Current opinion in cell biology.

[19]  P. Dunham,et al.  Characterization of the proteins of the intestinal Na(+)-K(+)-2Cl- cotransporter. , 1994, The American journal of physiology.

[20]  Emilio,et al.  ION TRANSPORT IN THE INTESTINE OF ANGUILLA ANGUILLA: GRADIENTS AND TRANSLOCATORS , 1994, The Journal of experimental biology.

[21]  W. S. Lee,et al.  Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney. , 1994, The Journal of biological chemistry.

[22]  J. A. Payne,et al.  Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[23]  F. Hossler,et al.  The gill arch of the striped bass (Morone saxatilis). IV. Alterations in the ultrastructure of chloride cell apical crypts and chloride efflux following exposure to seawater , 1991, Journal of morphology.

[24]  P. Hwang,et al.  Changes of plasma osmolality, chloride concentration and gill Na−K-ATPase activity in tilapia Oreochromis mossambicus during seawater acclimation , 1989 .

[25]  P. Prunet,et al.  Ultrastructural features of chloride cells in the gill epithelium of the Atlantic salmon, Salmo salar, and their modifications during smoltification. , 1988, The American journal of anatomy.

[26]  F. Hossler,et al.  The gill arch of the striped bass, Morone saxatilis. I: Surface ultrastructure , 1986 .

[27]  F. Epstein,et al.  Surface ultrastructure of the gill arch of the killifish, Fundulus heteroclitus, from seawater and freshwater, with special reference to the morphology of apical crypts of chloride cells , 1985, Journal of morphology.

[28]  J. Foskett,et al.  The chloride cell: definitive identification as the salt-secretory cell in teleosts. , 1982, Science.