Antibacterial properties of LDPE nanocomposite films in packaging of UF cheese

Abstract The purpose of this study was to investigate antibacterial potential of low-density polyethylene (LDPE) packaging films incorporating silver (Ag), copper oxide (CuO) and zinc oxide (ZnO) nanoparticles in measuring of coliform amounts of ultra-filtrated (UF) cheese. The initial LDPE/nanoparticle composites were produced by melting extrusion followed by extruding the obtained composites through a die to achieve a film thickness of 45 ± 5 μm. The number of surviving coliform bacteria was decreased by 4.21 log cfu/g after 4 weeks of storage at 4 ± 0.5 °C, whilst pure LDPE films showed a reduction of only 1.04 log cfu/g. A composition of 0% Ag, 1% CuO, 0% ZnO gave an optimum point in combined design using Design Expert analysis. A suitable microbial model was suggested for retarding coliform bacteria growth in UF cheese. The difference between the optimum point of nanocomposite film and its repeat was not significant (p

[1]  L. Ranjard,et al.  Differential copper impact on density, diversity and resistance of adapted culturable bacterial populations according to soil organic status. , 2010 .

[2]  Pierre Picouet,et al.  Metallic-based micro and nanocomposites in food contact materials and active food packaging , 2012 .

[3]  V. Pavlović,et al.  Development of low fat UF cheese technology , 2011 .

[4]  C. Tan,et al.  Melt Production and Antimicrobial Efficiency of Low-Density Polyethylene (LDPE)-Silver Nanocomposite Film , 2012, Food and Bioprocess Technology.

[5]  M. Fernández-García,et al.  Tailoring polymer–TiO2 film properties by presence of metal (Ag, Cu, Zn) species: Optimization of antimicrobial properties , 2011 .

[6]  H. M. Azeredo.  Nanocomposites for food packaging applications , 2009 .

[7]  Lina Ghibelli,et al.  Copper Nanoparticle/Polymer Composites with Antifungal and Bacteriostatic Properties , 2005 .

[8]  E. Cerda,et al.  Nanocomposites based on polyethylene and nanosilver particles produced by metallocenic “in situ” polymerization: synthesis, characterization, and antimicrobial behavior , 2011 .

[9]  A. Conte,et al.  Bio-based nanocomposite coating to preserve quality of Fior di latte cheese. , 2011, Journal of dairy science.

[10]  Ke Yang,et al.  Antibacterial Mechanism of Copper-bearing Antibacterial Stainless Steel against E:Coli , 2008 .

[11]  I. Sondi,et al.  Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. , 2004, Journal of colloid and interface science.

[12]  Helmut Münstedt,et al.  Silver ion release from antimicrobial polyamide/silver composites. , 2005, Biomaterials.

[13]  M. Shahedi,et al.  Effect of nanocomposite packaging containing Ag and ZnO on inactivation of Lactobacillus plantarum in orange juice , 2011 .

[14]  R. E. Smallman,et al.  An assessment of high voltage electron microscopy (HVEM). An invited review , 1977 .

[15]  Lucilla Iacumin,et al.  Effect of TiO2 photocatalytic activity in a HDPE-based food packaging on the structural and microbiological stability of a short-ripened cheese. , 2013, Food chemistry.

[16]  Paola Appendini,et al.  Review of antimicrobial food packaging , 2002 .

[17]  Aaron Brody,et al.  Active Packaging for Food Applications , 2001 .

[18]  W. Horwitz,et al.  Official methods of analysis of AOAC International , 2010 .

[19]  Younes Mostofi,et al.  Evaluation of the photocatalytic antimicrobial effects of a TiO2 nanocomposite food packaging film by in vitro and in vivo tests , 2013 .

[20]  Begoña Panea,et al.  Effect of nanocomposite packaging containing different proportions of ZnO and Ag on chicken breast meat quality , 2014 .

[21]  J. M. López-Vilariño,et al.  Determination of antioxidant migration levels from low-density polyethylene films into food simulants. , 2003, Journal of chromatography. A.

[22]  P. Espitia,et al.  Zinc Oxide Nanoparticles: Synthesis, Antimicrobial Activity and Food Packaging Applications , 2012, Food and Bioprocess Technology.

[23]  M. D. Del Nobile,et al.  Use of chitosan to prolong mozzarella cheese shelf life. , 2005, Journal of dairy science.

[24]  C. Nerín,et al.  Nanoparticle release from nano-silver antimicrobial food containers. , 2013, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[25]  P. Dubois,et al.  Polymer-layered silicate nanocomposites: preparation, properties and uses of a new class of materials , 2000 .

[26]  Zonghua Qin,et al.  The Effect of the Nano-ZnO Concentration on the Mechanical, Antibacterial and Melt Rheological Properties of LLDPE/Modified Nano-ZnO Composite Films , 2010 .

[27]  Milena Sinigaglia,et al.  Effect of Ag‐containing Nano‐composite Active Packaging System on Survival of Alicyclobacillus acidoterrestris , 2004 .

[28]  M. A. Nobile,et al.  Use of active compounds for prolonging the shelf life of mozzarella cheese , 2008 .

[29]  M. Shahedi,et al.  Evaluation of nanocomposite packaging containing Ag and ZnO on shelf life of fresh orange juice , 2010 .

[30]  Raija Ahvenainen,et al.  Novel food Packaging techniques , 2003 .