Relation between outer and luminal diameter in cannulated arteries.

Resistance in blood vessels is directly related to the inner (luminal) diameter (ID). However, ID can be difficult to measure during physiological experiments because of poor transillumination of thick-walled or tightly constricted vessels. We investigated whether the wall cross-sectional area (WCSA) in cannulated arteries is nearly constant, allowing IDs to be calculated from outer diameters (OD) using a single determination of WCSA. With the use of image analysis, OD and ID were directly measured using either transillumination or a fluorescent marker in the lumen. IDs from a variety of vessel types were calculated from WCSA at several reference pressures. Calculated IDs at all of the reference WCSA were within 5% (mean <1%) of the corresponding measured IDs in all vessel types studied, including vessels from heterozygote elastin knockout animals. This was true over a wide range of transmural pressures, during treatment with agonists, and before and after treatment with KCN. In conclusion, WCSA remains virtually constant in cannulated vessels, allowing accurate determination of ID from OD measurement under a variety of experimental conditions.

[1]  R. Mecham,et al.  Novel arterial pathology in mice and humans hemizygous for elastin. , 1998, The Journal of clinical investigation.

[2]  P. Challande,et al.  In vivo/in vitro comparison of rat abdominal aorta wall viscosity. Influence of endothelial function. , 1997, Arteriosclerosis, thrombosis, and vascular biology.

[3]  N. Stergiopulos,et al.  Contributions of vascular tone and structure to elastic properties of a medium-sized artery. , 1996, Hypertension.

[4]  F L Wuyts,et al.  Elastic properties of human aortas in relation to age and atherosclerosis: a structural model. , 1995, Physics in medicine and biology.

[5]  I. Davison,et al.  The structure and physical properties of invertebrate and primitive vertebrate arteries. , 1995, Journal of Experimental Biology.

[6]  W. Boyle,et al.  Endothelium‐independent Vasoconstricting and Vasodilating Actions of Halothane on Rat Mesenteric Resistance Blood Vessels , 1995, Anesthesiology.

[7]  F. Sellke,et al.  Heterogeneous Vasomotor Responses of Rabbit Coronary Microvessels to Isoflurane , 1994, Anesthesiology.

[8]  J. Michel,et al.  Effect of chronic ANG I-converting enzyme inhibition on aging processes. II. Large arteries. , 1994, The American journal of physiology.

[9]  J. Atkinson,et al.  Effect of chronic ANG I-converting enzyme inhibition on aging processes. III. Endothelial function of mesenteric arterial bed of rat. , 1994, The American journal of physiology.

[10]  G. Gamble,et al.  Estimation of Arterial Stiffness, Compliance, and Distensibility From M‐Mode Ultrasound Measurements of the Common Carotid Artery , 1994, Stroke.

[11]  M. Lew,et al.  Wall thickness to lumen diameter ratios of arteries from SHR and WKY: comparison of pressurised and wire-mounted preparations. , 1992, Journal of vascular research.

[12]  Y. Tardy,et al.  Effect of mental stress on the tone of a medium‐sized muscular artery , 1992, Journal of hypertension. Supplement : official journal of the International Society of Hypertension.

[13]  B. Starcher,et al.  Composition and mechanics of mesenteric resistance arteries from pregnant rats. , 1992, The American journal of physiology.

[14]  Y. Tardy,et al.  Non-invasive determination of arterial diameter and distensibility by echo-tracking techniques in hypertension , 1992, Journal of hypertension. Supplement : official journal of the International Society of Hypertension.

[15]  J. Spaan,et al.  Cannulation and continuous cross-sectional area measurement of small blood vessels. , 1990, Journal of pharmacological methods.

[16]  R N Vaishnav,et al.  Effect of hypertension on elasticity and geometry of aortic tissue from dogs. , 1990, Journal of biomechanical engineering.

[17]  G. Osol,et al.  Myogenic properties of cerebral blood vessels from normotensive and hypertensive rats. , 1985, The American journal of physiology.

[18]  S. Greenwald,et al.  Changes in the distensibility of the intrapulmonary arteries in the normal newborn and growing pig. , 1982, Cardiovascular research.

[19]  H. Handa,et al.  Experimental cerebral vasospasm arterial wall mechanics and connective tissue composition. , 1982, Stroke.

[20]  P. Duijvendijk,et al.  Cardiovascular physiology , 1976, Medical History.

[21]  M. Mulvany,et al.  Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. , 1977, Circulation research.

[22]  R N Vaishnav,et al.  Compressibility of the Arterial Wall , 1968, Circulation research.

[23]  S. Glagov,et al.  Structural Basis for the Static Mechanical Properties of the Aortic Media , 1964, Circulation research.

[24]  C. Wiederhielm.  CONTINUOUS RECORDING OF ARTERIOLAR DIMENSIONS WITH A TELEVISION MICROSCOPE. , 1963, Journal of applied physiology.

[25]  J. Nichol,et al.  The effect of cholesterol feeding on the distensibility of the isolated thoracic aorta of the rabbit. , 1955, Canadian journal of biochemistry and physiology.

[26]  I. C. Benson,et al.  STUDIES ON THE ELASTIC PROPERTIES OF HUMAN ISOLATED AORTA. , 1937, The Journal of clinical investigation.

[27]  H. Gregersen,et al.  Static elastic wall properties of the abdominal porcine aorta in vitro and in vivo. , 1997, European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery.

[28]  M. Cipolla,et al.  Pregnancy-induced changes in the three-dimensional mechanical properties of pressurized rat uteroplacental (radial) arteries. , 1993, American journal of obstetrics and gynecology.

[29]  B. Duling,et al.  Distribution of capillary blood flow in the microcirculation of the hamster: an in vivo study using epifluorescent microscopy. , 1984, Microvascular research.

[30]  Y C Fung,et al.  Compressibility and constitutive equation of arterial wall in radial compression experiments. , 1984, Journal of biomechanics.

[31]  J. Forrest,et al.  Comparison of blood vessel wall dimensions in normotensive hypertensive rats by histometric and morphometric methods. , 1983, Blood vessels.

[32]  E. Monos,et al.  Effect of acute ischaemia on active and passive large deformation mechanics of canine carotid arteries. , 1979, Acta physiologica Academiae Scientiarum Hungaricae.

[33]  Randy H. Kardon,et al.  Tissues and Organs: A Text-Atlas of Scanning Electron Microscopy , 1979 .

[34]  C. Roy,et al.  The Elastic Properties of the Arterial Wall , 1881, The Journal of physiology.