Optimization of Fermentation Condition of Chitinase-producing Bacterium C4 by Uniform-design

The effects of a number of factors on the production of chitinase were studied,including carbon source,nitrogen source,pH,volume of culture media,ultural temperature and time.The results showed that the optimal carbon source and nitrogen source were colloid chitin,KNO_3,and peptone,respectively.The optimization pH 7.6～8.5,cultural temperature:28℃,and C4 was cultivated for 5d on 180r/min shaker in flask with 12% volume medium.On the basis of this,the recipe of fermentation medium was optimized through uniform-design.A suitable fermentation condition was found for chitinase-producing strain C4.The optimal conditions were as follows:colloid chitin 2.5%,peptone 0.55%,KNO_3 0.3%,MgSO_4 0.09%,Tween 80 0.005%.Chitinase activity reaches 2.68U/mL under the conditions,bove 90.1% that of the basic fermentation conditions previously.