Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue: applications for diagnosis of oral precancer.

We present a Monte Carlo model to predict fluorescence spectra of the oral mucosa obtained with a depth-selective fiber optic probe as a function of tissue optical properties. A model sensitivity analysis determines how variations in optical parameters associated with neoplastic development influence the intensity and shape of spectra, and elucidates the biological basis for differences in spectra from normal and premalignant oral sites. Predictions indicate that spectra of oral mucosa collected with a depth-selective probe are affected by variations in epithelial optical properties, and to a lesser extent, by changes in superficial stromal parameters, but not by changes in the optical properties of deeper stroma. The depth selective probe offers enhanced detection of epithelial fluorescence, with 90% of the detected signal originating from the epithelium and superficial stroma. Predicted depth-selective spectra are in good agreement with measured average spectra from normal and dysplastic oral sites. Changes in parameters associated with dysplastic progression lead to a decreased fluorescence intensity and a shift of the spectra to longer emission wavelengths. Decreased fluorescence is due to a drop in detected stromal photons, whereas the shift of spectral shape is attributed to an increased fraction of detected photons arising in the epithelium.

[1]  Haishan Zeng,et al.  Simple device for the direct visualization of oral-cavity tissue fluorescence. , 2006, Journal of biomedical optics.

[2]  Michele Follen,et al.  Analytical model to describe fluorescence spectra of normal and preneoplastic epithelial tissue: comparison with Monte Carlo simulations and clinical measurements. , 2004, Journal of biomedical optics.

[3]  Nirmala Ramanujam,et al.  Effect of fiber optic probe geometry on depth-resolved fluorescence measurements from epithelial tissues: a Monte Carlo simulation. , 2003, Journal of biomedical optics.

[4]  H.J.C.M. Sterenborg,et al.  Skin optics , 1989, IEEE Transactions on Biomedical Engineering.

[5]  I. S. Saidi,et al.  Transcutaneous Optical Measurement of Hyperbilirubinemia in Neonates , 1992 .

[6]  G. Ogden,et al.  Apoptosis, proliferation, and angiogenesis in oral tissues. Possible relevance to tumour progression , 2000, The Journal of pathology.

[7]  Linda T. Nieman,et al.  Optical sectioning using a fiber probe with an angled illumination-collection geometry: evaluation in engineered tissue phantoms. , 2004, Applied optics.

[8]  K. Badizadegan,et al.  NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes. , 2002, Cancer research.

[9]  F. Janot,et al.  Autofluorescence videoendoscopy for photodiagnosis of head and neck squamous cell carcinoma , 2003, European Archives of Oto-Rhino-Laryngology.

[10]  Ashleyj . Welch,et al.  Optical-Thermal Response of Laser-Irradiated Tissue , 1995 .

[11]  Angela A. Eick,et al.  Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics. , 1998, Applied optics.

[12]  B Palcic,et al.  Optical properties of normal and carcinomatous bronchial tissue. , 1994, Applied optics.

[13]  Rebecca Richards-Kortum,et al.  Realistic three-dimensional epithelial tissue phantoms for biomedical optics. , 2002, Journal of biomedical optics.

[14]  Donald E Ingber,et al.  Cancer as a disease of epithelial-mesenchymal interactions and extracellular matrix regulation. , 2002, Differentiation; research in biological diversity.

[15]  Rebecca Richards-Kortum,et al.  Understanding the Biological Basis of Autofluorescence Imaging for Oral Cancer Detection: High-Resolution Fluorescence Microscopy in Viable Tissue , 2008, Clinical Cancer Research.

[16]  Džena Hidović-Rowe,et al.  Modelling and validation of spectral reflectance for the colon , 2005, Physics in medicine and biology.

[17]  B. Kulapaditharom,et al.  Performance Characteristics of Fluorescence Endoscope in Detection of Head and Neck Cancers , 2001, The Annals of otology, rhinology, and laryngology.

[18]  Rebecca Richards-Kortum,et al.  Autofluorescence and diffuse reflectance spectroscopy of oral epithelial tissue using a depth-sensitive fiber-optic probe. , 2008, Applied optics.

[19]  Calum MacAulay,et al.  Laser-induced fluorescence spectroscopy at endoscopy: tissue optics, Monte Carlo modeling, and in vivo measurements , 1995 .

[20]  Nirmala Ramanujam,et al.  Sequential estimation of optical properties of a two-layered epithelial tissue model from depth-resolved ultraviolet-visible diffuse reflectance spectra , 2006 .

[21]  Jarod C Finlay,et al.  Recovery of hemoglobin oxygen saturation and intrinsic fluorescence with a forward-adjoint model. , 2005, Applied optics.

[22]  G. Ogden,et al.  The association between tumour progression and vascularity in the oral mucosa , 1997, The Journal of pathology.

[23]  Rebecca Richards-Kortum,et al.  Light scattering from collagen fiber networks: micro-optical properties of normal and neoplastic stroma. , 2007, Biophysical journal.

[24]  Craig Gardner,et al.  Propagation of fluorescent light , 1997, Lasers in surgery and medicine.

[25]  J. Roodenburg,et al.  Autofluorescence and diffuse reflectance spectroscopy for oral oncology , 2005, Lasers in surgery and medicine.

[26]  Michele Follen,et al.  Sources of scattering in cervical tissue: determination of the scattering coefficient by confocal microscopy. , 2005, Applied optics.

[27]  G. Zonios,et al.  Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo. , 1999, Applied optics.

[28]  Lihong V. Wang,et al.  Monte Carlo Modeling of Light Transport in Tissues , 1995 .

[29]  Melissa C Skala,et al.  Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues. , 2005, Cancer research.

[30]  H Stepp,et al.  Autofluorescence imaging and spectroscopy of normal and malignant mucosa in patients with head and neck cancer , 1999, Lasers in surgery and medicine.

[31]  S. Shapshay,et al.  Spectroscopic detection and evaluation of morphologic and biochemical changes in early human oral carcinoma , 2003, Cancer.

[32]  R Richards-Kortum,et al.  Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: modeling, measurements, and implications. , 2001, Journal of biomedical optics.

[33]  Max J H Witjes,et al.  Clinical study for classification of benign, dysplastic, and malignant oral lesions using autofluorescence spectroscopy. , 2004, Journal of biomedical optics.

[34]  Michele Follen,et al.  Model-based analysis of clinical fluorescence spectroscopy for in vivo detection of cervical intraepithelial dysplasia. , 2006, Journal of biomedical optics.

[35]  Michele Follen,et al.  Spatially resolved reflectance spectroscopy for diagnosis of cervical precancer: Monte Carlo modeling and comparison to clinical measurements. , 2006, Journal of biomedical optics.

[36]  R Richards-Kortum,et al.  Detection and diagnosis of oral neoplasia with an optical coherence microscope. , 2004, Journal of biomedical optics.

[37]  Rebecca Richards-Kortum,et al.  Reflectance spectroscopy for diagnosis of epithelial precancer: model-based analysis of fiber-optic probe designs to resolve spectral information from epithelium and stroma. , 2005, Applied optics.

[38]  R. Richards-Kortum,et al.  Optimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy¶ , 2000, Photochemistry and photobiology.