Introduction to Transmission and Scanning Electron Microscopy

This chapter is a modest attempt to present the basic techniques in both transmission and scanning work. The recommendation outlined in this chapter are likely to be considered standard protocol. As new specific techniques are developed or older once evolved the literature aimed at solving specific problems should be considered.

[1]  P. Raymond,et al.  Improved method for obtaining 3-microns cryosections for immunocytochemistry. , 1990, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[2]  J R Smith,et al.  Hydrogen ion buffers for biological research. , 1966, Analytical biochemistry.

[3]  G. Bullock,et al.  Techniques in Immunocytochemistry , 1983 .

[4]  A. J. Wilson,et al.  Procedures in electron microscopy , 1993 .

[5]  J. Koehler,et al.  Advanced Techniques in Biological Electron Microscopy , 1974 .

[6]  W. Reville,et al.  An evaluation of the usefulness of air-drying biological samples from tetramethylsilane in preparation for scanning electron microscopy. , 1991, Journal of electron microscopy.

[7]  M. Karnovsky,et al.  THE ULTRASTRUCTURAL BASIS OF CAPILLARY PERMEABILITY STUDIED WITH PEROXIDASE AS A TRACER , 1967, The Journal of cell biology.

[8]  Kéiichi Tanaka,et al.  A method for observing intracellular structures of free cells by scanning electron microscopy , 1986, Journal of microscopy.

[9]  L. Barsanti,et al.  Ultrastructure of the apical zone of Euglena gracilis: photoreceptors and motor apparatus. , 1991, Electron microscopy reviews.

[10]  D. Knight,et al.  Staining methods for sectioned material , 1977 .

[11]  A. Glauert,et al.  A New Embedding Medium for Electron Microscopy , 1956, Nature.

[12]  F. Verni,et al.  Peculiar epibionts in Euplotidium itoi (ciliata, hypotrichida) , 1990 .

[13]  J. Luft.  Embedding Media — Old and New , 1973 .

[14]  Russell J. Barrnett,et al.  Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. , 1963 .