Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates.

Abstract Loach ( Misgurnus anguillicaudatus ) proteins were hydrolysed by papain and Protamex, the antioxidant activity of loach protein hydrolysates (LPH) was investigated. The results demonstrated that extensive hydrolysis by papain and Protamex led to the browning of the hydrolysates. When the degree of hydrolysis (DH) was 23%, hydrolysates prepared by papain (HA) exhibited the strongest antioxidant activity. The maximum values of the hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activities and the reducing power were 56.1%, 95.5%, 2.80 mM and 1.46, respectively. The hydrolysates prepared by Protamex (HB) showed the strongest hydroxyl radical scavenging activity (55.0%) at DH 28%, DPPH radical scavenging activity (92.2%) and ABTS radical scavenging activity (2.81 mM) at DH 23%, and the reducing power (1.17) at DH 33%. At the same DH value, there were significant ( p Industrial relevance Loach has long been employed as a traditional Chinese medicine for the treatment of many kinds of diseases. From our previous work, loach was determined to be a good source of protein (accounts for approximately 17% (w/w) of the body weight). In this work, loach proteins were hydrolyzed by papain and Protamex to specific extent. The effect of DH on the antioxidant activities of hydrolysates was investigated. The results indicated that loach protein hydrolysates were potent antioxidants which were significantly affected by DH. This research is helpful for extensive development of loach product.

[1]  E. Li-Chan,et al.  Functional Properties of Fish Protein Hydrolysate from Herring (Clupea harengus) , 1999 .

[2]  Dongfeng Wang,et al.  Antioxidant and biochemical properties of protein hydrolysates prepared from Silver carp (Hypophthalmichthys molitrix) , 2008 .

[3]  Chyuan-Yuan Shiau,et al.  Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus) , 2003 .

[4]  H. Suh,et al.  Antioxidant activities of five different mulberry cultivars in Korea , 2007 .

[5]  Won‐Kyo Jung,et al.  Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties , 2005 .

[6]  B. Rasco,et al.  Biochemical and functional properties of Atlantic salmon (Salmo salar) muscle proteins hydrolyzed with various alkaline proteases. , 2000, Journal of agricultural and food chemistry.

[7]  M. Almajano,et al.  Changes in the antioxidant properties of protein solutions in the presence of epigallocatechin gallate , 2007 .

[8]  J. Terao,et al.  Antioxidant and emulsifying activity of N-(long-chain-acyl)histidine and N-(long-chain-acyl)carnosine , 1993 .

[9]  C. B. Park,et al.  A novel antimicrobial peptide from the loach, Misgurnus anguillicaudatus , 1997, FEBS letters.

[10]  Kaixun Huang,et al.  Protective effect of polysaccharide from the loach on the in vitro and in vivo peroxidative damage of hepatocyte. , 2002, The Journal of nutritional biochemistry.

[11]  K. Uchida,et al.  Sequence-dependent reactivity of histidine-containing peptides with copper(II)/ascorbate , 1992 .

[12]  S. Benjakul,et al.  Protein Hydrolysates from Pacific Whiting Solid Wastes , 1997 .

[13]  M. Boekel.  Effect of heating on Maillard reactions in milk. , 1998 .

[14]  K. Nokihara,et al.  Antioxidant activity of designed peptides based on the antioxidative peptide isolated from digests of a soybean protein , 1996 .

[15]  J. Parajó,et al.  Antioxidant properties of ultrafiltration-recovered soy protein fractions from industrial effluents and their hydrolysates , 2006 .

[16]  T. Sérot,et al.  Study of the interaction of fish myosin with the products of lipid oxidation: The case of aldehydes , 2007 .

[17]  Y. Xiong,et al.  Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato protein is related to its reducing and radical scavenging ability. , 2005, Journal of agricultural and food chemistry.

[18]  M. Shahedi,et al.  Antioxidant activity of Kelussia odoratissima Mozaff. in model and food systems , 2007 .

[19]  Xiaoxiong Zeng,et al.  Optimization of extraction and isolation for 11S and 7S globulins of soybean seed storage protein , 2007 .

[20]  F. Shahidi,et al.  ANTIOXIDATIVE ACTIVITY OF PROTEIN HYDROLYSATE FROM ROUND SCAD MUSCLE USING ALCALASE AND FLAVOURZYME , 2007 .

[21]  M. Yamazaki,et al.  Induction of release of cytotoxin from murine bone marrow cells by an animal lectin. , 1987, Cancer research.

[22]  B. Ames,et al.  Oxidants, antioxidants, and the degenerative diseases of aging. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[23]  M. Suphantharika,et al.  Optimization of enzymatic hydrolysis of fish soluble concentrate by commercial proteases , 2005 .

[24]  John Shi,et al.  Effect of ultrasonic treatment on the recovery and DPPH radical scavenging activity of polysaccharides from longan fruit pericarp , 2008 .

[25]  Fereidoon Shahidi,et al.  Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type , 2007 .

[26]  Chenxiaoxue Zhang,et al.  Mechanism of apoptosis induced by a polysaccharide, from the loach Misgurnus anguillicaudatus (MAP) in human hepatocellular carcinoma cells. , 2006, Toxicology and applied pharmacology.

[27]  M. Asakawa,et al.  Characterization of a deaminated neuraminic acid-containing glycoprotein from the skin mucus of the loach, Misgurnus anguillicaudatus. , 1994, The Journal of biological chemistry.

[28]  D J McClements,et al.  Mechanisms of the antioxidant activity of a high molecular weight fraction of whey. , 2000, Journal of agricultural and food chemistry.

[29]  P. Park,et al.  Antioxidant activity of a peptide isolated from Alaska pollack (Theragra chalcogramma) frame protein hydrolysate , 2005 .

[30]  T. Osawa,et al.  Hydroxyl Radical-scavenging Effects of Spices and Scavengers from Brown Mustard (Brassica nigra) , 1997 .

[31]  P. Venskutonis,et al.  Screening of radical scavenging activity of some medicinal and aromatic plant extracts , 2004 .

[32]  J. Synowiecki,et al.  Production and characteristics of protein hydrolysates from capelin (Mallotus villosus) , 1995 .

[33]  K. Shimada,et al.  Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion , 1992 .

[34]  M. Cacciuttolo,et al.  Hyperoxia induces DNA damage in mammalian cells. , 1993, Free radical biology & medicine.