Protein Hydrolysis Degree in Vitro and in Vivo Protein Digestibility of Four Autoclaved Tropical Legume Seeds

The objective of this study was to evaluate the digestibility of crude protein in vivo (DCP) and the protein hydrolysis degree (PHD) of Mucunapruriens (MP), Cajanuscajan (CC), Phaseoluslunatus (PL) and Vignaunguiculata (VU) seeds, which were autoclavedat different times. The difference between the PHDand DCP of theevaluated legumeswas significant (P<0.05). Mucunapruriens had the highest PHD (5.74%) followed by VU (4.55%), CC (4.42%) and PL (2.45%). In addition, VU (80.46%) hadthe greatest DCP followed by CC (69.92%), PL (67.14%) and MP (61.51%). For all legumes, the PHDand DCP significantly increased (P<0.05)with an increase in autoclaving time. The results indicate that an increase in autoclaving time correlated to an increase in DCP and PHDforthe evaluated legumes. However, the PHDof MP and PL did not correspond to the DCP results.

[1]  E. Njagi,et al.  Effects of Processing Mucuna Bean (Mucuna pruriens L.) on Protein Quality and Antinutrients Content , 2012 .

[2]  G. Kaushik,et al.  Processing of food legumes: a boon to human nutrition , 2010 .

[3]  M. Pugalenthi,et al.  EFFECT OF VARIOUS PROCESSING METHODS ON THE LEVELS OF ANTINUTRITIONAL CONSTITUENTS AND PROTEIN DIGESTIBILITY OF MUCUNA PRURIENS (L.) DC. VAR. UTILIS (WALL. EX WIGHT) BAKER EX BURCK (VELVET BEAN) SEEDS , 2008 .

[4]  F. .,et al.  Antinutrient Factors of Vegetable Cowpea (Sesquipedalis) Seeds During Thermal Processing , 2007 .

[5]  D. Thangadurai CHEMICAL COMPOSITION AND NUTRITIONAL POTENTIAL OF VIGNA UNGUICULATA SSP. CYLINDRICA (FABACEAE) , 2005 .

[6]  Jessé A. Pacheco Aguirre,et al.  Extracción y caracterización de las fracciones proteínicas solubles del grano de Phaseolus lunatus L , 2004 .

[7]  E. Adeparusi Effect of processing on the nutrients and anti-nutrients of lima bean (Phaseolus lunatus L.) flour. , 2001, Die Nahrung.

[8]  K. Janardhanan,et al.  Studies on chemical composition and antinutritional factors in three germplasm seed materials of the tribal pulse, Mucuna pruriens (L.) DC , 1992 .

[9]  J. Adler-Nissen,et al.  Enzymic Hydrolysis of Food Proteins , 1986 .

[10]  K. Ganzler,et al.  Simple Enzymic Methods for Prediction of Plant Protein Digestibility , 1985 .

[11]  B. Eggum,et al.  Factors affecting the protein quality of pigeonpea (Cajanus cajan L.) , 1984 .

[12]  W. Flatt,et al.  the Evaluation of Feeds through Digestibility Experiments , 1975 .