New Murine Monoclonal Antibodies Directed against Glycophorins C and D, Have Anti‐Ge2 Specificity

Background and Objectives: The Gerbich blood group system comprises three high‐incidence antigens which have been localized on glycophorins C (GPC) and D (GPD) molecules. Murine monoclonal antibodies (mAbs) directed against Ge4 and Ge3 epitopes have already been obtained. We describe two murine mAbs (Z151.17 and J6) with Ge2 specificity. Materials and Methods: The mAbs were investigated by serological methods and immunoblotting, with common, Ge‐negative and enzyme‐modified RBCs. Results: Both mAbs behaved like anti‐Ge2 in serological tests, but bound to GPC and GPD on immunoblots. Z151.17 did not recognize the GPCYus or GPCGe variants, whereas J6 revealed a 25‐kD component from Ge:‐2,‐3,4 membranes, which is identical to the GPCGe molecule. Conclusion: These two mAbs are directed to slightly different epitopes. The Z151.17 and J6 epitopes are common to GPC and GPD, thus distinct from the Ge2 antigen defined by human alloantibodies.

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