MICROTUBULES IN THE FORMATION AND DEVELOPMENT OF THE PRIMARY MESENCHYME IN ARBACIA PUNCTULATA

To experimentally test the suggestion made in the preceding paper that the microtubules are involved in cell shape development during the formation and differentiation of the primary mesenchyme, we applied to the embryos two types of agents which affect cytoplasmic microtubules: (a) colchicine and hydrostatic pressure, which cause the microtubules to disassemble, and (b) D2O, which tends to stabilize them. When the first type of agent is applied to sea urchin gastrulae, the development of the primary mesenchyme ceases, the microtubules disappear, and the cells tend to spherulate. With D2O development also ceases, but the tubules appear "frozen," and the cell asymmetries persist unaltered. These agents appear to block development by primarily interfering with the sequential disassembly and/or reassembly of microtubules into new patterns. The microtubules, therefore, appear to be influential in the development of cell form. On the other hand through a careful analysis of the action of these agents and others on both intra- and extracellular factors, we concluded that the microtubules do rather little for the maintenance of cell shape in differentiated tissues.

[1]  J. Gibbins,et al.  MICROTUBULES IN THE FORMATION AND DEVELOPMENT OF THE PRIMARY MESENCHYME IN ARBACIA PUNCTULATA , 1969, The Journal of cell biology.

[2]  K. Porter,et al.  STUDIES ON THE MICROTUBULES IN HELIOZOA , 1967, The Journal of cell biology.

[3]  R. Kane.  THE MITOTIC APPARATUS , 1967, The Journal of cell biology.

[4]  D. Marsland,et al.  Counteraction of the anti-mitotic effects of D20 in the dividing eggs of Argacia punctulata: a temperature-pressure analysis. , 1966, Experimental cell research.

[5]  D. Marsland.  Anti‐mitotic effects of colchicine and hydrostatic pressure; synergistic action on the cleaving eggs of Lytechinus variegatus , 1966, Journal of cellular physiology.

[6]  S. Malawista.  ON THE ACTION OF COLCHICINE , 1965, The Journal of experimental medicine.

[7]  M. Rudzinska.  THE FINE STRUCTURE AND FUNCTION OF THE TENTACLE IN TOKOPHRYA INFUSIONUM , 1965, The Journal of cell biology.

[8]  D. Marsland,et al.  STRUCTURAL STABILIZATION OF THE MITOTIC APPARATUS BY HEAVY WATER, IN THE CLEAVING EGGS OF ARBACIA PUNCTULATA; INCREASED RESISTANCE TO PRESSURE-INDUCED DISORGANIZATION. , 1965, Experimental cell research.

[9]  V. Nachmias,et al.  CELL SURFACE AND PINOCYTOSIS , 1965, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  G. de‐Thé,et al.  CYTOPLASMIC MICROTUBULES IN DIFFERENT ANIMAL CELLS , 1964, The Journal of cell biology.

[11]  K. Porter,et al.  ORIENTED MICROTUBULES IN ELONGATING CELLS OF THE DEVELOPING LENS RUDIMENT AFTER INDUCTION. , 1964, Proceedings of the National Academy of Sciences of the United States of America.

[12]  D. Marsland,et al.  CELL DIVISION: EFFECTS OF PRESSURE ON THE MITOTIC MECHANISMS OF MARINE EGGS (ARBACIA PUNCTULATA). , 1964, Experimental cell research.

[13]  N. Gonatas,et al.  THE ULTRASTRUCTURE OF A MAMMALIAN CELL DURING THE MITOTIC CYCLE , 1964, The Journal of cell biology.

[14]  K. Porter,et al.  A "MICROTUBULE" IN PLANT CELL FINE STRUCTURE , 1963, The Journal of cell biology.

[15]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[16]  D. Marsland,et al.  Cell division: Differential effects of heavy water upon the mechanisms of cytokinesis and karyokinesis in the eggs of Arbacia punctulata , 1963 .

[17]  P B Green,et al.  Mechanism for Plant Cellular Morphogenesis , 1962, Science.

[18]  L. Thibodeau,et al.  The micromorphology of Amoeba proteus during pressure-induced changes in the sol-gel cycle. , 1962, Experimental cell research.

[19]  P. Harris.  SOME STRUCTURAL AND FUNCTIONAL ASPECTS OF THE MITOTIC APPARATUS IN SEA URCHIN EMBRYOS , 1962, The Journal of cell biology.

[20]  John H. Luft,et al.  IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS , 1961, The Journal of biophysical and biochemical cytology.

[21]  P. Gross,et al.  HEAVY WATER INHIBITION OF CELL DIVISION: AN APPROACH TO MECHANISM * , 1960, Annals of the New York Academy of Sciences.

[22]  M. L. Watson.  Staining of Tissue Sections for Electron Microscopy with Heavy Metals , 1958, The Journal of biophysical and biochemical cytology.

[23]  D. Marsland.  The mechanisms of cell division; temperature-pressure experiments on the cleaving eggs of Arbacia punctulata. , 1950, Journal of cellular and comparative physiology.

[24]  D. Pease.  Hydrostatic pressure effects upon the spindle figure and chromosome movement; experiments on the meiotic divisions of Trade-scantia pollen mother cells. , 1946, The Biological bulletin.

[25]  D. Marsland.  MECHANISM OF PIGMENT DISPLACEMENT IN UNICELLULAR CHROMATOPHORES , 1944 .

[26]  D. Pease,et al.  The liquefaction of the tentacles of suctorian protozoa at high hydrostatic pressures , 1939 .

[27]  D. Marsland.  The mechanism of protoplasmic streaming. The effects of high hydrostatic pressure upon cyclosis in Elodea canadensis , 1939 .

[28]  M. Aspiz,et al.  [Cytoplasmic microtubules]. , 1970, Uspekhi sovremennoi biologii.

[29]  L. E. Roth.  Motile Systems with Continuous Filaments1 , 1964 .

[30]  K. Wohlfarth-Bottermann.  Differentiations of the Ground Cytoplasm and Their Significance for the Generation of the Motive Force of Ameboid Movement1 , 1964 .

[31]  D. Pitelka.  Electron-microscopic Structure of Protozoa , 1963 .

[32]  E. Daniels,et al.  Electron microscopic studies of mitosis in amebae. II. The giant ameba Pelomyxa carolinensis. , 1962 .

[33]  D. Marsland.  Protoplasmic Contractility in Relation to Gel Structure: Temperature – Pressure Experiments on Cytokinesis and Amoeboid Movement , 1956 .

[34]  E. Wilson,et al.  The Cell In Development And Heredity , 1925 .