Separation and Purification of dl-Tetrahydropalmatine from Corydalis Yanhusuo W. T. Wang by HSCCC with a New Solvent System Screening Method

Abstract A fast and simple solvent system screening method was established based on average polarity, which was much easier to find a suitable solvent system for high speed countercurrent chromatography separation. Dl-tetrahydropalmatine was separated and purified in one step with n-hexane-ethyl acetate-methanol-water (4-6-5-5) on high speed countercurrent chromatography. Acetic acid was successfully used to adjust the pH value of the sample solution to improve recovery of the separation. As a result, 4.37 mg dl-tetrahydropalmatine was separated from 25 mg crude extract at the purity of 92.7% with the recovery of 95.1%.

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