Berry ripening: recently heard through the grapevine.

Grapevine (Vitis vinifera L.) is a non-climacteric fruit species used as table fruit, dried raisins, and for vinification (wines) and distillation (liquors). In recent years, our knowledge of the molecular basis of ripening regulation has improved. Water status, light conditions, and temperature may hasten, delay, or enhance ripening. Hormones seem to play a central role, as their concentrations change prior to and during ripening and in response to several environmental cues. The review summarizes recent data related to the molecular and hormonal control of grape berry development and ripening, with special emphasis on secondary metabolism and its response to the environment, and pinpoints some experimental limitations.

[1]  Jungmin Lee,et al.  Influence of grapevine leafroll associated viruses (GLRaV-2 and -3) on the fruit composition of Oregon Vitis vinifera L. cv. Pinot noir: Phenolics , 2009 .

[2]  D. Severac,et al.  Stimulation of the grape berry expansion by ethylene and effects on related gene transcripts, over the ripening phase. , 2008, Physiologia plantarum.

[3]  C. Pratt Reproductive Anatomy in Cultivated Grapes - A Review , 1971, American Journal of Enology and Viticulture.

[4]  D. Dubourdieu,et al.  A New Type of Flavor Precursors in Vitis vinifera L. cv. Sauvignon Blanc: S-Cysteine Conjugates , 1998 .

[5]  R. Gutiérrez,et al.  Compatible GLRaV-3 viral infections affect berry ripening decreasing sugar accumulation and anthocyanin biosynthesis in Vitis vinifera , 2011, Plant Molecular Biology.

[6]  T. Ferrier,et al.  The Transcription Factor VvMYB5b Contributes to the Regulation of Anthocyanin and Proanthocyanidin Biosynthesis in Developing Grape Berries12[W] , 2008, Plant Physiology.

[7]  P. Piccoli,et al.  Solar UV-B and ABA are involved in phenol metabolism of Vitis vinifera L. increasing biosynthesis of berry skin polyphenols. , 2011, Journal of agricultural and food chemistry.

[8]  Hirohiko Hirochika,et al.  Retrotransposon-Induced Mutations in Grape Skin Color , 2004, Science.

[9]  J. Luczo,et al.  Transcriptional regulation of the three grapevine chalcone synthase genes and their role in flavonoid synthesis in Shiraz , 2013 .

[10]  M. M. Chaves,et al.  Characterization of mitochondrial dicarboxylate/tricarboxylate transporters from grape berries , 2012, Planta.

[11]  A. Velders,et al.  Flavonol 3-O-glycosides series of Vitis vinifera Cv. Petit Verdot red wine grapes. , 2009, Journal of agricultural and food chemistry.

[12]  J. Tregear,et al.  SSR markers in transcripts of genes linked to post-transcriptional and transcriptional regulatory functions during vegetative and reproductive development of Elaeis guineensis , 2012, BMC Plant Biology.

[13]  L. Bavaresco,et al.  Phenolics in Grape Berry and Key Antioxidants , 2012 .

[14]  Mei Zhang,et al.  Reciprocity between abscisic acid and ethylene at the onset of berry ripening and after harvest , 2010, BMC Plant Biology.

[15]  M. Jiménez,et al.  Monoterpenes in grape juice and wines. , 2000, Journal of chromatography. A.

[16]  J. Bohlmann,et al.  Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries. , 2004, Phytochemistry.

[17]  R. Takata,et al.  Analysis of S-3-(Hexan-1-ol)-Glutathione and S-3-(Hexan-1-ol)-l-Cysteine in Vitis vinifera L. cv. Koshu for Aromatic Wines , 2010, American Journal of Enology and Viticulture.

[18]  J. Maga,et al.  Pyrazines in foods: an update. , 1982, Critical reviews in food science and nutrition.

[19]  A. S. Duque,et al.  Cell suspension cultures , 2006 .

[20]  G. Conéjéro,et al.  In vivo grapevine anthocyanin transport involves vesicle-mediated trafficking and the contribution of anthoMATE transporters and GST. , 2011, The Plant journal : for cell and molecular biology.

[21]  Mark A. Matthews,et al.  Water deficits accelerate ripening and induce changes in gene expression regulating flavonoid biosynthesis in grape berries , 2007, Planta.

[22]  M. Esaka,et al.  Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins , 2004 .

[23]  M. Shiraishi,et al.  Evaluation of table grape genetic resources for sugar, organic acid, and amino acid composition of berries , 2010, Euphytica.

[24]  H. Xin,et al.  Anthocyanin profile and gene expression in berry skin of two red Vitis vinifera grape cultivars that are sunlight dependent versus sunlight independent , 2013 .

[25]  Shaohua Li,et al.  Proteomic analysis of grape berry skin responding to sunlight exclusion. , 2013, Journal of plant physiology.

[26]  N. Goto-Yamamoto,et al.  Abscisic acid stimulated ripening and gene expression in berry skins of the Cabernet Sauvignon grape , 2010, Functional & Integrative Genomics.

[27]  E. Gomès,et al.  The grape berry-specific basic helix–loop–helix transcription factor VvCEB1 affects cell size , 2013, Journal of experimental botany.

[28]  B. Burla,et al.  The multidrug resistance‐associated protein (MRP/ABCC) subfamily of ATP‐binding cassette transporters in plants , 2006, FEBS letters.

[29]  J. Raynal,et al.  Exogenous ethylene stimulates the long-term expression of genes related to anthocyanin biosynthesis in grape berries , 2003 .

[30]  Diane M. Martin,et al.  Biosynthesis of wine aroma: transcript profiles of hydroxymethylbutenyl diphosphate reductase, geranyl diphosphate synthase, and linalool/nerolidol synthase parallel monoterpenol glycoside accumulation in Gewürztraminer grapes , 2012, Planta.

[31]  D. Greer,et al.  Heat stress affects flowering, berry growth, sugar accumulation and photosynthesis of Vitis vinifera cv. Semillon grapevines grown in a controlled environment , 2010 .

[32]  S. Conn,et al.  Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins , 2008, Journal of experimental botany.

[33]  Leanne Webb,et al.  Modelled impact of future climate change on the phenology of winegrapes in Australia , 2007 .

[34]  R. Stracke,et al.  The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries1[W][OA] , 2009, Plant Physiology.

[35]  P. Marquet,et al.  Climate change, wine, and conservation , 2013, Proceedings of the National Academy of Sciences.

[36]  J. Santiago,et al.  Identification of and relationships among a number of teinturier grapevines that expanded across Europe in the early 20th century , 2008 .

[37]  S. Zhong,et al.  Single-base resolution methylomes of tomato fruit development reveal epigenome modifications associated with ripening , 2013, Nature Biotechnology.

[38]  Seth D. Cohen,et al.  Assessing the impact of temperature on grape phenolic metabolism. , 2008, Analytica chimica acta.

[39]  C. R. Hale,et al.  The hormone content of ripening grape berries and the effects of growth substance treatments. , 1973, Plant physiology.

[40]  A. Razungles,et al.  Varietal thiols in wine: discovery, analysis and applications. , 2011, Chemical reviews.

[41]  P. Boss,et al.  Analysis of the Expression of Anthocyanin Pathway Genes in Developing Vitis vinifera L. cv Shiraz Grape Berries and the Implications for Pathway Regulation , 1996, Plant physiology.

[42]  K. Hashizume,et al.  Purification and Characterization of a O-Methyltransferase Capable of Methylating 2-Hydroxy-3-alkylpyrazine from Vitis vinifera L. (cv. Cabernet Sauvignon) , 2001, Bioscience, biotechnology, and biochemistry.

[43]  R. Dixon,et al.  The Mysteries of Proanthocyanidin Transport and Polymerization1 , 2010, Plant Physiology.

[44]  S. Kallithraka,et al.  Irrigation and rootstock effects on the phenolic concentration and aroma potential of Vitis vinifera L. cv. cabernet sauvignon grapes. , 2009, Journal of agricultural and food chemistry.

[45]  K. Hashizume,et al.  S-Adenosyl-L-methionine-dependent O-Methylation of 2-Hydroxy-3-alkylpyrazine in Wine Grapes: A Putative Final step of Methoxypyrazine Biosynthesis , 2001, Bioscience, biotechnology, and biochemistry.

[46]  B. M. Lange,et al.  Genome organization in Arabidopsis thaliana: a survey for genes involved in isoprenoid and chlorophyll metabolism , 2003, Plant Molecular Biology.

[47]  J. Cushman,et al.  Water Deficit Increases Stilbene Metabolism in Cabernet Sauvignon Berries , 2010, Journal of agricultural and food chemistry.

[48]  D. Merdinoglu,et al.  Four specific isogenes of the anthocyanin metabolic pathway are systematically co-expressed with the red colour of grape berries , 2006 .

[49]  J. Todd,et al.  Polymorphism discovery and association analyses of the interferon genes in type 1 diabetes , 2006, BMC Genetics.

[50]  M. Kitayama,et al.  Effects of abscisic acid treatment and night temperatures on anthocyanin composition in pinot noir grapes , 2015 .

[51]  P. Boss,et al.  A methyltransferase essential for the methoxypyrazine-derived flavour of wine. , 2013, The Plant journal : for cell and molecular biology.

[52]  M. Downey,et al.  The effect of bunch shading on berry development and flavonoid accumulation in Shiraz grapes , 2008 .

[53]  K. Geoffrey White,et al.  The distinctive flavour of New Zealand Sauvignon blanc: Sensory characterisation by wine professionals , 2007 .

[54]  M. M. Chaves,et al.  Impact of irrigation regime on berry development and flavonoids composition in Aragonez (Syn. Tempranillo) grapevine , 2012 .

[55]  Danijela Poljuha and Barbara Sladonja The Mediterranean Genetic Code - Grapevine and Olive , 2013 .

[56]  M. Pezzotti,et al.  Skin pectin metabolism during the postharvest dehydration of berries from three distinct grapevine cultivars , 2013 .

[57]  M. Morgante,et al.  Expansion and subfunctionalisation of flavonoid 3',5'-hydroxylases in the grapevine lineage , 2010, BMC Genomics.

[58]  J. Kopka,et al.  Search for Transcriptional and Metabolic Markers of Grape Pre-Ripening and Ripening and Insights into Specific Aroma Development in Three Portuguese Cultivars , 2013, PloS one.

[59]  N. Goto-Yamamoto,et al.  Organ-Specific Transcription of Putative Flavonol Synthase Genes of Grapevine and Effects of Plant Hormones and Shading on Flavonol Biosynthesis in Grape Berry Skins , 2006, Bioscience, biotechnology, and biochemistry.

[60]  E. Gomès,et al.  Metabolic profiling reveals coordinated switches in primary carbohydrate metabolism in grape berry (Vitis vinifera L.), a non-climacteric fleshy fruit , 2013, Journal of experimental botany.

[61]  M. Matthews,et al.  Impact of diurnal temperature variation on grape berry development, proanthocyanidin accumulation, and the expression of flavonoid pathway genes , 2012, Journal of experimental botany.

[62]  M. Esaka,et al.  Expression of multi-copy flavonoid pathway genes coincides with anthocyanin, flavonol and flavan-3-ol accumulation of grapevine , 2015 .

[63]  H. Wada,et al.  Fruit ripening in Vitis vinifera: spatiotemporal relationships among turgor, sugar accumulation, and anthocyanin biosynthesis , 2011, Journal of experimental botany.

[64]  Jungmin Lee,et al.  Influence of grapevine leafroll associated viruses (GLRaV-2 and -3) on the fruit composition of Oregon Vitis vinifera L. cv. Pinot noir: Free amino acids, sugars, and organic acids , 2009 .

[65]  Matthew W. Fidelibus,et al.  Effects of Forchlorfenuron and Abscisic Acid on the Quality of ‘Flame Seedless’ Grapes , 2008 .

[66]  Jungmin Lee,et al.  Berry Temperature and Solar Radiation Alter Acylation, Proportion, and Concentration of Anthocyanin in Merlot Grapes , 2008, American Journal of Enology and Viticulture.

[67]  Julie M. Tarara,et al.  Separation of Sunlight and Temperature Effects on the Composition of Vitis vinifera cv. Merlot Berries , 2002, American Journal of Enology and Viticulture.

[68]  I. S. Pretorius,et al.  Genetic Determinants of Volatile-Thiol Release by Saccharomyces cerevisiae during Wine Fermentation , 2005, Applied and Environmental Microbiology.

[69]  K. Hirano,et al.  Effects of water deficit stress on leaf and berry ABA and berry ripening in Chardonnay grapevines ( Vitis vinifera ) , 2015 .

[70]  Gregory M Symons,et al.  Grapes on Steroids. Brassinosteroids Are Involved in Grape Berry Ripening1 , 2005, Plant Physiology.

[71]  L. Williams,et al.  2-Methoxy-3-isobutylpyrazine in grape berries and its dependence on genotype. , 2010, Phytochemistry.

[72]  A. Ageorges,et al.  Grapevine MATE-Type Proteins Act as Vacuolar H+-Dependent Acylated Anthocyanin Transporters1[W][OA] , 2009, Plant Physiology.

[73]  A. Gallois,et al.  Study of the biosynthesis of 3-isopropyl-2-methoxypyrazine produced by Pseudomonas taetrolens , 1988 .

[74]  Masahiko Kitayama,et al.  Loss of anthocyanins in red-wine grape under high temperature. , 2007, Journal of experimental botany.

[75]  M. S. Grando,et al.  The 1-deoxy-d-xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine , 2009, Theoretical and Applied Genetics.

[76]  Shaohua Li,et al.  Sugar and acid concentrations in 98 grape cultivars analyzed by principal component analysis , 2006 .

[77]  G. Mazza,et al.  Anthocyanins, phenolics, and color of Cabernet Franc, Merlot, and Pinot Noir wines from British Columbia. , 1999, Journal of agricultural and food chemistry.

[78]  Shan Chen,et al.  Anthocyanin Accumulation in Various Organs of a Teinturier Cultivar (Vitis vinifera L.) during the Growing Season , 2012, American Journal of Enology and Viticulture.

[79]  D. Merdinoglu,et al.  Variation of linalool and geraniol content within two pairs of aromatic and non-aromatic grapevine clones , 2009 .

[80]  Diane M. Martin,et al.  Identification of Vitis vinifera (-)-alpha-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase. , 2004, Phytochemistry.

[81]  E. Gomès,et al.  VvGOLS1 and VvHsfA2 are involved in the heat stress responses in grapevine berries. , 2012, Plant & cell physiology.

[82]  Vasil Georgiev,et al.  Production of Anthocyanins in Grape Cell Cultures: A potential Source of Raw Material for Pharmaceutical, Food, and Cosmetic Industries , 2013 .

[83]  C. Ford,et al.  The relationship between the expression of abscisic acid biosynthesis genes, accumulation of abscisic acid and the promotion of Vitis vinifera L. berry ripening by abscisic acid. , 2009 .

[84]  T. Kubo,et al.  ABA and sugar effects on anthocyanin formation in grape berry cultured in vitro , 2001 .

[85]  M. M. Chaves,et al.  ABCC1, an ATP Binding Cassette Protein from Grape Berry, Transports Anthocyanidin 3-O-Glucosides[W][OA] , 2013, Plant Cell.

[86]  E. Gomès,et al.  Genetic Analysis of the Biosynthesis of 2-Methoxy-3-Isobutylpyrazine, a Major Grape-Derived Aroma Compound Impacting Wine Quality1[W] , 2013, Plant Physiology.

[87]  Yumiko Suzuki,et al.  Environmental stress enhances biosynthesis of flavor precursors, S-3-(hexan-1-ol)-glutathione and S-3-(hexan-1-ol)-L-cysteine, in grapevine through glutathione S-transferase activation , 2010, Journal of experimental botany.

[88]  N. Goto-Yamamoto,et al.  Effects of Temperature on Anthocyanin Biosynthesis in Grape Berry Skins , 2006, American Journal of Enology and Viticulture.

[89]  S. Delrot,et al.  The basic helix-loop-helix transcription factor MYC1 is involved in the regulation of the flavonoid biosynthesis pathway in grapevine. , 2010, Molecular plant.

[90]  J. T. Matus,et al.  Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes , 2008, BMC Plant Biology.

[91]  Fangfei Luan,et al.  Differential incorporation of 1-deoxy-D-xylulose into (3S)-linalool and geraniol in grape berry exocarp and mesocarp. , 2002, Phytochemistry.

[92]  E. Peterlunger,et al.  Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin , 2006, BMC Genomics.

[93]  M. Cutanda-Perez,et al.  Ectopic expression of VlmybA1 in grapevine activates a narrow set of genes involved in anthocyanin synthesis and transport , 2009, Plant Molecular Biology.

[94]  C. Triggs,et al.  New Zealand Sauvignon blanc Distinct Flavor Characteristics: Sensory, Chemical, and Consumer Aspects , 2009, American Journal of Enology and Viticulture.

[95]  C. Ford,et al.  Transcriptome analysis at four developmental stages of grape berry (Vitis vinifera cv. Shiraz) provides insights into regulated and coordinated gene expression , 2012, BMC Genomics.

[96]  P. Boss,et al.  Treatment of Grape Berries, a Nonclimacteric Fruit with a Synthetic Auxin, Retards Ripening and Alters the Expression of Developmentally Regulated Genes , 1997, Plant physiology.

[97]  Jérôme Grimplet,et al.  Water deficit alters differentially metabolic pathways affecting important flavor and quality traits in grape berries of Cabernet Sauvignon and Chardonnay , 2009, BMC Genomics.

[98]  M. Allen,et al.  Contribution of Methoxypyrazines to Sauvignon blanc Wine Aroma , 1991, American Journal of Enology and Viticulture.

[99]  S. Delrot,et al.  Proteomic analysis of the effects of ABA treatments on ripening Vitis vinifera berries , 2010, Journal of experimental botany.

[100]  V. Sadras,et al.  Effects of elevated temperature in grapevine. I Berry sensory traits , 2013 .

[101]  D. Dubourdieu,et al.  The Role of Yeasts in Grape Flavor Development during Fermentation: The Example of Sauvignon blanc , 2006, American Journal of Enology and Viticulture.

[102]  F. Barrieu,et al.  Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures. , 2008, Plant physiology and biochemistry : PPB.

[103]  P. Boss,et al.  Sequestration of auxin by the indole-3-acetic acid-amido synthetase GH3-1 in grape berry (Vitis vinifera L.) and the proposed role of auxin conjugation during ripening. , 2010, Journal of experimental botany.

[104]  G. Sacks,et al.  Behavior of 3-isobutyl-2-hydroxypyrazine (IBHP), a key intermediate in 3-isobutyl-2-methoxypyrazine (IBMP) metabolism, in ripening wine grapes. , 2012, Journal of agricultural and food chemistry.

[105]  A. R. Walker,et al.  The Grapevine Transcription Factor VvMYBPA1 Regulates Proanthocyanidin Synthesis during Fruit Development1[OA] , 2007, Plant Physiology.

[106]  M. Walker,et al.  Application of abscisic acid rapidly upregulated UFGT gene expression and improved color of grape berries , 2015 .

[107]  Fei He,et al.  Biosynthesis of Anthocyanins and Their Regulation in Colored Grapes , 2010, Molecules.

[108]  E. Agosin,et al.  Methoxypyrazines in Grapes and Wines of Vitis vinifera cv. Carmenere , 2007, American Journal of Enology and Viticulture.

[109]  V. G. Jaudzems,et al.  Morphology, anatomy and development of the pericarp after anthesis in grape, Vitis vinifera L. , 1996 .

[110]  T. Lacombe,et al.  Wine grape (Vitis vinifera L.) color associates with allelic variation in the domestication gene VvmybA1 , 2007, Theoretical and Applied Genetics.

[111]  S. Conn,et al.  Characterization of anthocyanic vacuolar inclusions in Vitis vinifera L. cell suspension cultures , 2010, Planta.

[112]  B. Donèche,et al.  Effect of Esca disease on the phenolic and sensory attributes of Cabernet Sauvignon grapes, musts and wines , 2012 .

[113]  Diane M. Martin,et al.  Functional Annotation, Genome Organization and Phylogeny of the Grapevine (Vitis vinifera) Terpene Synthase Gene Family Based on Genome Assembly, FLcDNA Cloning, and Enzyme Assays , 2010, BMC Plant Biology.

[114]  M. Esaka,et al.  Expression of the flavonoid 3′-hydroxylase and flavonoid 3′,5′-hydroxylase genes and flavonoid composition in grape (Vitis vinifera) , 2006 .

[115]  Z. Singh,et al.  Girdling and grapevine leafroll associated viruses affect berry weight, colour development and accumulation of anthocyanins in ‘Crimson Seedless’ grapes during maturation and ripening , 2008 .

[116]  Benhong Wu,et al.  Anthocyanin composition and content in grape berry skin in Vitis germplasm , 2008 .

[117]  Carlos Conde,et al.  Biochemical Changes throughout Grape Berry Development and Fruit and Wine Quality , 2007 .

[118]  N. Goto-Yamamoto,et al.  Bunch Shading During Different Developmental Stages Affects the Phenolic Biosynthesis in Berry Skins of ‘Cabernet Sauvignon’ Grapes , 2008 .

[119]  R. Bottini,et al.  Water deficit and exogenous ABA significantly affect grape and wine phenolic composition under in field and in-vitro conditions , 2011, Plant Growth Regulation.

[120]  C. Forde,et al.  Auxin treatment of pre‐veraison grape (Vitis vinifera L.) berries both delays ripening and increases the synchronicity of sugar accumulation , 2011 .

[121]  S. Lund,et al.  The Molecular Basis for Wine Grape Quality-A Volatile Subject , 2006, Science.

[122]  C. Bonghi,et al.  Grape berry ripening delay induced by a pre-véraison NAA treatment is paralleled by a shift in the expression pattern of auxin- and ethylene-related genes , 2012, BMC Plant Biology.

[123]  L. Gény,et al.  Involvement of Abscisic Acid in Controlling the Proanthocyanidin Biosynthesis Pathway in Grape Skin: New Elements Regarding the Regulation of Tannin Composition and Leucoanthocyanidin Reductase (LAR) and Anthocyanidin Reductase (ANR) Activities and Expression , 2010, Journal of Plant Growth Regulation.

[124]  J. Cavagnaro,et al.  Abscisic acid is involved in the response of grape (Vitis vinifera L.) cv. Malbec leaf tissues to ultraviolet-B radiation by enhancing ultraviolet-absorbing compounds, antioxidant enzymes and membrane sterols. , 2009, Plant, cell & environment.

[125]  S. Delrot,et al.  The biochemistry of the grape berry , 2012 .

[126]  D. Dubourdieu,et al.  3-Sulfanylhexanol precursor biogenesis in grapevine cells: the stimulating effect of Botrytis cinerea. , 2011, Journal of agricultural and food chemistry.

[127]  S. Ebeler,et al.  Wine chemistry and flavor: looking into the crystal glass. , 2009, Journal of agricultural and food chemistry.

[128]  G. Di Gaspero,et al.  Expression of flavonoid genes in the red grape berry of ‘Alicante Bouschet’ varies with the histological distribution of anthocyanins and their chemical composition , 2012, Planta.

[129]  V. Sadras,et al.  Elevated temperature decouples anthocyanins and sugars in berries of Shiraz and Cabernet Franc , 2012 .

[130]  J. T. Matus,et al.  Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera , 2009, Journal of experimental botany.

[131]  S. Delrot,et al.  Why climate change will not dramatically decrease viticultural suitability in main wine-producing areas by 2050 , 2013, Proceedings of the National Academy of Sciences.

[132]  K. Gindro,et al.  Impact of clonal variability in Vitis vinifera Cabernet franc on grape composition, wine quality, leaf blade stilbene content, and downy mildew resistance. , 2013, Journal of agricultural and food chemistry.

[133]  V. Walbot,et al.  AN9, a petunia glutathione S-transferase required for anthocyanin sequestration, is a flavonoid-binding protein. , 2000, Plant physiology.

[134]  Rubén Cañedo Andalia,et al.  Bentham Science Publishers , 2008 .

[135]  J. Roustan,et al.  Ethylene seems required for the berry development and ripening in grape, a non-climacteric fruit , 2004 .

[136]  D. Dubourdieu,et al.  Sulfur aroma precursor present in S-glutathione conjugate form: identification of S-3-(hexan-1-ol)-glutathione in must from Vitis vinifera L. cv. Sauvignon blanc. , 2002, Journal of agricultural and food chemistry.

[137]  V. Lauvergeat,et al.  Characterization of a Grapevine R2R3-MYB Transcription Factor That Regulates the Phenylpropanoid Pathway1[W] , 2005, Plant Physiology.

[138]  P. Boss,et al.  Acyl substrate preferences of an IAA-amido synthetase account for variations in grape (Vitis vinifera L.) berry ripening caused by different auxinic compounds indicating the importance of auxin conjugation in plant development , 2011, Journal of experimental botany.

[139]  B. Bartolomé,et al.  Vitis vinifera L. cv. Graciano grapes characterized by its anthocyanin profile , 2004 .

[140]  B. Wilson,et al.  Nonvolatile Conjugates of Secondary Metabolites as Precursors of Varietal Grape Flavor Components , 1989 .

[141]  A. McElrone,et al.  Sugar and abscisic acid signaling orthologs are activated at the onset of ripening in grape , 2010, Planta.

[142]  D. Merdinoglu,et al.  Genetic variability of descriptors for grapevine berry acidity in Riesling, Gewürztraminer and their progeny , 2014 .

[143]  K. Yazaki Transporters of secondary metabolites. , 2005, Current opinion in plant biology.

[144]  J. Aguirreolea,et al.  Thermotolerance responses in ripening berries of Vitis vinifera L. cv Muscat Hamburg. , 2013, Plant & cell physiology.

[145]  Christian Kappel,et al.  Ecophysiological, Genetic, and Molecular Causes of Variation in Grape Berry Weight and Composition: A Review , 2011, American Journal of Enology and Viticulture.

[146]  Florian F. Bauer,et al.  Wine flavor and aroma , 2011, Journal of Industrial Microbiology & Biotechnology.

[147]  A. Azuma,et al.  Flavonoid biosynthesis-related genes in grape skin are differentially regulated by temperature and light conditions , 2012, Planta.

[148]  C. Ford,et al.  Regulation of Malate Metabolism in Grape Berry and Other Developing Fruits , 2009 .

[149]  E Bombardelli,et al.  VITIS VINIFERA L , 1995 .

[150]  R. Ming,et al.  The effects of artificial selection on sugar metabolism and transporter genes in grape , 2013, Tree Genetics & Genomes.

[151]  J. T. Matus,et al.  Isolation of WDR and bHLH genes related to flavonoid synthesis in grapevine (Vitis vinifera L.) , 2010, Plant Molecular Biology.

[152]  D. Dubourdieu,et al.  Location of 2-Methoxy-3-isobutylpyrazine in Cabernet Sauvignon Grape Bunches and Its Extractability during Vinification , 2002, American Journal of Enology and Viticulture.