Cytology, RAPD, and seed colour of progeny plants from Brassica rapa-alboglabra aneuploids and development of monosomic addition lines.

Progeny plants from Brassica rapa-alboglabra aneuploids were characterized genetically by scoring random amplified polymorphic DNA (RAPD) markers and seed colour and cytologically as to chromosome number and pairing. Sets of RAPD markers specific for each of the encountered eight alien Brassica alboglabra chromosomes were defined. The finding of subsets of markers associated with the presence or absence of alien chromosomes inferred the frequent occurrence of intergenomic genetic recombination and introgression. The chromosome numbers were in the range 2n = 20-28, with a maximum of seven alien B. alboglabra chromosomes and one trisomic B. rapa chromosome. Five types of monosomic addition lines were obtained, two of which have not been developed before. Differences in chromatin condensation patterns made it possible to differentiate between the B. rapa and B. alboglabra chromosomes at diakinesis, and to detect intergenomic homoeological pairing. In addition to the frequent formation of trivalents by homoeologous pairing of an alien B. alboglabra chromosome and a background B. rapa pair, occasional heteromorphic intergenomic bivalents and B. rapa univalents were encountered. Homoeological intergenomic pairing occurred between chromosomes with similar centromeric and karyotypic positions. Plants with structurally changed alien chromosomes were found. The RAPD and cytological data substantiated each other. Observations of the colour of sown and harvested seeds indicated that B. alboglabra chromosome 4 carries a gene for brown seed colour. It exerts its control embryonically, and thus it differs from chromosome 1 which controls seed colour maternally.

[1]  K. Brismar,et al.  Maternal and embryonal control of seed colour by different Brassica alboglabra chromosomes , 2001 .

[2]  C. Quirós,et al.  DNA-based marker maps of Brassica , 2001 .

[3]  W. Köhler,et al.  Molecular Cytogenetic Localization and Characterization of 5S and 25S rDNA Loci for Chromosome Identification in Oilseed Rape (Brassica napus L.) , 2000 .

[4]  S. Bang,et al.  Early-bolting trait and RAPD markers in the specific monosomic addition line of radish carrying the e-chromosome of Brassica oleracea , 2000 .

[5]  G. King,et al.  An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations , 2000, Theoretical and Applied Genetics.

[6]  D. Marshall,et al.  Physical mapping of DNA repetitive sequences to mitotic and meiotic chromosomes of Brassica oleracea var. alboglabra by fluorescence in situ hybridization , 1998, Heredity.

[7]  P. Kirti,et al.  Generation and characterisation of monosomic chromosome addition lines of Brassica campestris - B. oxyrrhina , 1998, Theoretical and Applied Genetics.

[8]  Jinguo Hu,et al.  Linkage group alignment from four independent Brassica oleracea RFLP maps , 1998 .

[9]  K. Fukui,et al.  Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45s rDNA loci on the identified chromosomes , 1998, Theoretical and Applied Genetics.

[10]  W. Köhler,et al.  Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids , 1997, Theoretical and Applied Genetics.

[11]  W. Köhler,et al.  Chromosomal localization and characterization of rDNA loci in the Brassica A and C genomes. , 1997, Genome.

[12]  R. Jørgensen,et al.  Production and cytogenetics of Brassica campestris-alboglabra chromosome addition lines , 1997, Theoretical and Applied Genetics.

[13]  A. Chèvre,et al.  Identification of the different Brassica nigra chromosomes from both sets of B. oleracea-B. nigra and B. napus-B. nigra addition lines with a special emphasis on chromosome transmission and self-incompatibility , 1997, Theoretical and Applied Genetics.

[14]  B. Landry,et al.  Comparison of the genetic maps of Brassica napus and Brassica oleracea , 1997, Theoretical and Applied Genetics.

[15]  P. This,et al.  Characterization of Brassica nigra chromosomes and of blackleg resistance in B. napus–B. nigra addition lines , 1996 .

[16]  A. Sharpe,et al.  Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). , 1995, Genome.

[17]  P. Lu,et al.  Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[18]  W. Heneen,et al.  Mitotic karyotypes of Brassica campestris and Brassica alboglabra and identification of the B. alboglabra chromosome in an addition line. , 1995, Genome.

[19]  W. Heneen,et al.  Meiotic studies on a Brassica campestris-alboglabra monosomic addition line and derived B. campestris primary trisomics. , 1994, Genome.

[20]  W. Heneen,et al.  Addition of Brassica alboglabra Bailey chromosomes to B. campestris L. with special emphasis on seed colour , 1994, Heredity.

[21]  A. Chèvre,et al.  Interspecific hybrids between a transgenic rapeseed (Brassica napus) and related species: cytogenetical characterization and detection of the transgene. , 1993, Genome.

[22]  D. Struss,et al.  Studies on resistance to Phoma lingam in Brassica napus-Brassica nigra addition lines. , 1993 .

[23]  U. Lagercrantz,et al.  Mapping the Brassica Genome , 1993 .

[24]  D. Struss,et al.  Mapping of Molecular Markers on Brassica B‐Genome Chromosomes Added to Brassica napus , 1992 .

[25]  P. This,et al.  Dissection of the Brassica nigra Genome by Monosomic Addition Lines , 1990 .

[26]  J. McGrath,et al.  Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility, and chromosome transmission , 1990 .

[27]  J. McGrath,et al.  Development and chromosomal localization of genome-specific DNA markers of Brassica and the evolution of amphidiploids and n = 9 diploid species , 1990 .

[28]  W. Heneen,et al.  Resynthesis of Brassies napus L. through Interspecific Hybridization between B. alboglabra Bailey and B. campestris L. with Special Emphasis on Seed Colour , 1988 .

[29]  W. Tai,et al.  Incomplete bivalent pairing in dihaploids of Brassica napus L , 1988 .

[30]  C. Busso,et al.  Digenomic triploids for an assessment of chromosome relationships in the cultivated diploid Brassica species , 1987 .

[31]  G. Röbbelen,et al.  Cytogenetic relationship within cultivated Brassica analyzed in amphihaploids from the three diploid ancestors , 1986 .

[32]  M. Shirzadegan.  Inheritance of seed color in Brassica napus L. , 1986 .

[33]  K. Hinata,et al.  Taxonomy, cytogenetics and origin of crop brassicas, a review. , 1980 .