Enterotoxigenic Profiles of Food-Poisoning and Food-Borne Bacillus cereus Strains

ABSTRACT The enterotoxigenic profiles of 51 B. cereus food-related strains were compared to those of 37 B. cereus food-poisoning strains. cytK and association of hbl-nhe-cytK enterotoxin genes were more frequent among diarrheal strains (73 and 63%) than among food-borne strains (37 and 33%). Unlike diarrheal strains, food-borne strains showed frequent nhe and hbl gene polymorphisms and were often low toxin producers.

[1]  P. E. Granum,et al.  Psychrotolerant species from the Bacillus cereus group are not necessarily Bacillus weihenstephanensis. , 2001, FEMS microbiology letters.

[2]  K. Wernars,et al.  Detection of genes encoding for enterotoxins and determination of the production of enterotoxins by HBL blood plates and immunoassays of psychrotrophic strains of Bacillus cereus isolated from pasteurised milk. , 2001, International journal of food microbiology.

[3]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[4]  N. Hendriksen,et al.  Detection of Enterotoxic Bacillus cereus andBacillus thuringiensis Strains by PCR Analysis , 2001, Applied and Environmental Microbiology.

[5]  P. E. Granum,et al.  A new cytotoxin from Bacillus cereus that may cause necrotic enteritis , 2000, Molecular microbiology.

[6]  P. E. Granum,et al.  Common occurrence of enterotoxin genes and enterotoxicity in Bacillus thuringiensis. , 2000, FEMS microbiology letters.

[7]  P. E. Granum,et al.  The sequence of the non-haemolytic enterotoxin operon from Bacillus cereus. , 1999, FEMS microbiology letters.

[8]  S. H. Beattie,et al.  Detection of toxigenic strains of Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay , 1999, Letters in applied microbiology.

[9]  E. Aranda,et al.  Detection of Clostridium botulinum types A, B, E and F in foods by PCR and DNA probe , 1997, Letters in applied microbiology.

[10]  B. Zilinskas,et al.  Molecular cloning and characterization of the genes encoding the L1 and L2 components of hemolysin BL from Bacillus cereus , 1997, Journal of bacteriology.

[11]  D. J. Beecher,et al.  Tripartite Hemolysin BL from Bacillus cereus , 1997, The Journal of Biological Chemistry.

[12]  M. Mori,et al.  The bceT gene of Bacillus cereus encodes an enterotoxic protein. , 1995, Microbiology.

[13]  P. E. Granum.  Bacillus cereus and its toxins. , 1994, Society for Applied Bacteriology symposium series.

[14]  D. J. Beecher,et al.  Improved purification and characterization of hemolysin BL, a hemolytic dermonecrotic vascular permeability factor from Bacillus cereus , 1994, Infection and immunity.

[15]  J. Heinrichs,et al.  Molecular cloning and characterization of the hblA gene encoding the B component of hemolysin BL from Bacillus cereus , 1993, Journal of bacteriology.