Combinational light emitting diode-high frequency focused ultrasound treatment for HeLa cell

Abstract Purpose: Light sources such as laser and light emitting diode or ultrasound devices have been widely used for cancer therapy and regenerative medicines, since they are more cost-effective and less harmful than radiation therapy, chemotherapy or magnetic treatment. Compared to laser and low intensity ultrasound techniques, light emitting diode and high frequency focused ultrasound shows enhanced therapeutic effects, especially for small tumors. Materials and methods: We propose combinational light emitting diode-high frequency focused ultrasound treatment for human cervical cancer HeLa cells. Individual red, green, and blue light emitting diode light only, high frequency focused ultrasound only, or light emitting diode light combined with high frequency focused ultrasound treatments were applied in order to characterize the responses of HeLa cells. Results: Cell density exposed by blue light emitting diode light combined with high frequency focused ultrasound (2.19 ± 0.58%) was much lower than that of cells exposed by red and green light emitting diode lights (81.71 ± 9.92% and 61.81 ± 4.09%), blue light emitting diode light (11.19 ± 2.51%) or high frequency focused ultrasound only (9.72 ± 1.04%). Conclusions: We believe that the proposed combinational blue light emitting diode-high frequency focused ultrasound treatment could have therapeutic benefits to alleviate cancer cell proliferation

[1]  E. Ben-hur,et al.  The phthalocyanines: a new class of mammalian cells photosensitizers with a potential for cancer phototherapy. , 1985, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[2]  S. Takeuchi,et al.  Basic study on apoptosis induction into cancer cells U-937 and EL-4 by ultrasound exposure. , 2006, Ultrasonics.

[3]  G. Farhat,et al.  Diagnostic ultrasound Imaging : Inside out , 2004 .

[4]  D. Kripke,et al.  Phototherapy for Depressive Disorders: A Review * , 1989, Canadian journal of psychiatry. Revue canadienne de psychiatrie.

[5]  Kwangmeyung Kim,et al.  Therapeutic Ultrasound Contrast Agents for the Enhancement of Tumor Diagnosis and Tumor Therapy. , 2015, Journal of biomedical nanotechnology.

[6]  Derek Abbott,et al.  Surface Roughness Detection of Arteries via Texture Analysis of Ultrasound Images for Early Diagnosis of Atherosclerosis , 2013, PloS one.

[7]  Stefano Geuna,et al.  Phototherapy for enhancing peripheral nerve repair: A review of the literature , 2005, Muscle & nerve.

[8]  P. Beard.  Biomedical photoacoustic imaging , 2011, Interface Focus.

[9]  Myung-Hee Sohn,et al.  Effect of blue light emitting diodes on melanoma cells: involvement of apoptotic signaling. , 2015, Journal of photochemistry and photobiology. B, Biology.

[10]  Kee W. Jang,et al.  The effect of low-intensity pulsed ultrasound on chondrocyte migration and its potential for the repair of articular cartilage , 2011 .

[11]  F. Dunn,et al.  Ultrasonic Scattering in Biological Tissues , 1992 .

[12]  R. Jain,et al.  Photodynamic therapy for cancer , 2003, Nature Reviews Cancer.

[13]  Ji-Bin Liu,et al.  Contrast-Enhanced Ultrasound , 2015, BioMed research international.

[14]  Se-woon Choe,et al.  Drug-loaded sickle cells programmed ex vivo for delayed hemolysis target hypoxic tumor microvessels and augment tumor drug delivery. , 2013, Journal of controlled release : official journal of the Controlled Release Society.

[15]  R. Apfel,et al.  Gauging the likelihood of cavitation from short-pulse, low-duty cycle diagnostic ultrasound. , 1991, Ultrasound in medicine & biology.

[16]  Michael C. Kolios,et al.  Properties of cells through life and death – an acoustic microscopy investigation , 2015, Cell cycle.

[17]  Tom F. Sheahan,et al.  A novel L1 retrotransposon marker for HeLa cell line identification. , 2009, BioTechniques.

[18]  D. H. Mash,et al.  Light-emitting diodes , 1977, Nature.

[19]  Noel T. Whelan,et al.  Effect of NASA light-emitting diode irradiation on wound healing. , 2001, Journal of clinical laser medicine & surgery.

[20]  Masaichi-chang-il Lee,et al.  Reactive oxygen species production in mitochondria of human gingival fibroblast induced by blue light irradiation. , 2013, Journal of photochemistry and photobiology. B, Biology.

[21]  J. Hwang,et al.  Therapeutic potential of ultrasound microbubbles in gastrointestinal oncology: recent advances and future prospects , 2015, Therapeutic advances in gastroenterology.

[22]  Bensheng Qiu,et al.  Therapeutic ultrasonic microbubbles carrying paclitaxel and LyP-1 peptide: preparation, characterization and application to ultrasound-assisted chemotherapy in breast cancer cells. , 2011, Ultrasound in medicine & biology.

[23]  R. Dawe,et al.  Incidence of skin cancers in 3867 patients treated with narrow‐band ultraviolet B phototherapy , 2008, The British journal of dermatology.

[24]  T. Delaney,et al.  Photodynamic therapy of cancer. , 1988, Comprehensive therapy.

[25]  Jong Seob Jeong,et al.  Design and characterization of dual-curvature 1.5-dimensional high-intensity focused ultrasound phased-array transducer , 2012, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[26]  Chandra M Sehgal,et al.  A review of low-intensity ultrasound for cancer therapy. , 2015, Ultrasound in medicine & biology.

[27]  K. Kirk Shung,et al.  Cell membrane deformation induced by a fibronectin-coated polystyrene microbead in a 200-MHz acoustic trap , 2014, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control.

[28]  C. Pinfildi,et al.  Low-level laser therapy and light-emitting diode effects in the secretion of neuropeptides SP and CGRP in rat skin , 2014, Lasers in Medical Science.