Age-related alterations in the activation of heat shock transcription factor 1 in rat hepatocytes.

The induction of hsp70 transcription by heat shock is significantly reduced in hepatocytes isolated from old rats compared to hepatocytes isolated from young/adult rats, and the decline in hsp70 transcription is correlated with a decrease in the induction of heat shock transcription factor 1 (HSF1) binding to the heat shock element. However, the decreased HSF1 binding activity to DNA is not due to reduced levels of HSF1 that are available for activation by heat shock. In fact, the levels of HSF1 are two- to threefold higher in hepatocytes from old rats, and the age-related increase in the levels of HSF1 protein in hepatocytes appears to arise from a decrease in the degradation of the HSF1 because HSF1 mRNA levels do not change and the synthesis of HSF1 decreases approximately 50% with age. No evidence was found for an impairment in HSF1 oligomerization in hepatocytes from old rats, e.g., the level of HSF1 trimers, the nuclear translocation of HSF1, and the phosphorylation of HSF1 after heat shock are similar in hepatocytes isolated from young/adult and old rats. However, the thermostability of the DNA binding activity of HSF1 was significantly reduced with age in a cell-free system as well as in isolated hepatocytes.

[1]  M. Pahlavani,et al.  Expression of heat shock protein 70 decreases with age in hepatocytes and splenocytes from female rats , 1999, Mechanisms of Ageing and Development.

[2]  A. Richardson,et al.  The expression of heat shock protein 70 decreases with cellular senescence in vitro and in cells derived from young and old human subjects. , 1998, Experimental cell research.

[3]  A. Gafni.  Structural Modifications of Proteins During Aging , 1997, Journal of the American Geriatrics Society.

[4]  R. Tanguay,et al.  Diminished heat shock response in the aged myocardium , 1996 .

[5]  N. Holbrook,et al.  Cellular aging and cell death , 1996 .

[6]  R. Kingston,et al.  The regulatory domain of human heat shock factor 1 is sufficient to sense heat stress , 1996, Molecular and cellular biology.

[7]  R. Morimoto,et al.  Activation of Heat Shock Factor 1 DNA Binding Precedes Stress-induced Serine Phosphorylation , 1996, The Journal of Biological Chemistry.

[8]  R. Weindruch,et al.  The expression of heat shock protein 70 decreases with age in lymphocytes from rats and rhesus monkeys. , 1995, Experimental cell research.

[9]  T. W. Fawcett,et al.  Effects of neurohormonal stress and aging on the activation of mammalian heat shock factor 1. , 1994, The Journal of biological chemistry.

[10]  J. Lis,et al.  Protein traffic on the heat shock promoter: Parking, stalling, and trucking along , 1993, Cell.

[11]  B. Wu,et al.  Expression of heat shock protein 70 is altered by age and diet at the level of transcription , 1993, Molecular and cellular biology.

[12]  R. Baler,et al.  Activation of human heat shock genes is accompanied by oligomerization, modification, and rapid translocation of heat shock transcription factor HSF1 , 1993, Molecular and cellular biology.

[13]  R. Morimoto,et al.  Cells in stress: transcriptional activation of heat shock genes. , 1993, Science.

[14]  B. Wu,et al.  The effect of age on the synthesis of two heat shock proteins in the hsp70 family. , 1993, Journal of gerontology.

[15]  R. Morimoto,et al.  Activation of Heat Shock Gene Transcription by Heat Shock Factor 1 Involves Oligomerization, Acquisition of DNA-Binding Activity, and Nuclear Localization and Can Occur in the Absence of Stress , 1993, Molecular and cellular biology.

[16]  J. Raese,et al.  Hsp70 mRNA induction is reduced in neurons of aged rat hippocampus after thermal stress , 1992, Neurobiology of Aging.

[17]  P. Petronini,et al.  Decreased Expression of Heat Shock Protein 70 mRNA and Protein in WI‐38 Human Fibroblasts Aging in Vitro , 1992, Annals of the New York Academy of Sciences.

[18]  Hueng-Sik Choi,et al.  Molecular events involved in transcriptional activation of heat shock genes become progressively refractory to heat stimulation during aging of human diploid fibroblasts , 1991, Journal of cellular physiology.

[19]  W. Ward,et al.  Effect of age on liver protein synthesis and degradation , 1991, Hepatology.

[20]  R. Morimoto,et al.  Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability. , 1991, Genes & development.

[21]  J. Fargnoli,et al.  Concomitant decline in heat-induced hyperthermia and HSP70 mRNA expression in aged rats. , 1991, The American journal of physiology.

[22]  Peter K. Sorger,et al.  Yeast heat shock factor contains separable transient and sustained response transcriptional activators , 1990, Cell.

[23]  P. Starke-Reed,et al.  Protein oxidation and proteolysis during aging and oxidative stress. , 1989, Archives of biochemistry and biophysics.

[24]  A. Richardson,et al.  Analysis of effect of age on synthesis of specific proteins by hepatocytes , 1989, Journal of cellular physiology.

[25]  J. Bautista,et al.  Implication of lysine residues in the loss of enzymatic activity in rat liver 6-phosphogluconate dehydrogenase found in aging. , 1989, The Journal of biological chemistry.

[26]  K. Rodysill,et al.  Diminished heat-shock protein synthesis following mitogen stimulation of lymphocytes from aged donors. , 1989, Experimental cell research.

[27]  B. Li,et al.  Attenuated induction of heat shock gene expression in aging diploid fibroblasts. , 1989, The Journal of biological chemistry.

[28]  S. Kishimoto,et al.  Age-related changes of heat shock protein gene transcription in human peripheral blood mononuclear cells. , 1988, Biochemical and biophysical research communications.

[29]  H. Pelham,et al.  Constitutive binding of yeast heat shock factor to DNA in vivo , 1988, Molecular and cellular biology.

[30]  P. Sorger,et al.  Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation , 1988, Cell.

[31]  J. Bautista,et al.  Possible involvement of histidine residues in the loss of enzymatic activity of rat liver malic enzyme during aging. , 1988, The Journal of biological chemistry.

[32]  A. Richardson,et al.  Effect of age and dietary restriction on the expression of alpha 2u-globulin. , 1987, The Journal of biological chemistry.

[33]  E. Stadtman,et al.  Age-related changes in oxidized proteins. , 1987, The Journal of biological chemistry.

[34]  R. Kingston,et al.  Heat-inducible human factor that binds to a human hsp70 promoter , 1987, Molecular and cellular biology.

[35]  M. Rothstein,et al.  Altered brain phosphoglycerate kinase from aging rats , 1984, Mechanisms of Ageing and Development.

[36]  L. S. Hiremath,et al.  The effect of aging on rat liver phosphoglycerate kinase and comparison with the muscle enzyme. , 1982, Biochimica et biophysica acta.

[37]  S B Thacker,et al.  Morbidity and mortality associated with the July 1980 heat wave in St Louis and Kansas City, Mo. , 1982, JAMA.

[38]  M. Rothstein.  The formation of altered enzymes in aging animals , 1979, Mechanisms of Ageing and Development.

[39]  G. L. Coleman,et al.  Pathological Changes During Aging in, Barrier-Reared Fischer 344 Male Rats1 , 1977, Journal of gerontology.

[40]  A. Richardson,et al.  The effect of age on protein synthesis and ribosome aggregation to messenger RNA in rat liver. , 1976, Archives of biochemistry and biophysics.

[41]  L. Orgel,et al.  The maintenance of the accuracy of protein synthesis and its relevance to ageing. , 1963, Proceedings of the National Academy of Sciences of the United States of America.

[42]  A. Richardson,et al.  Effect of caloric restriction on the expression of heat shock protein 70 and the activation of heat shock transcription factor 1. , 1996, Developmental genetics.

[43]  D. Reinberg,et al.  Common themes in assembly and function of eukaryotic transcription complexes. , 1995, Annual review of biochemistry.

[44]  S. Lindquist,et al.  The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. , 1993, Annual review of genetics.

[45]  R. Conaway,et al.  General initiation factors for RNA polymerase II. , 1993, Annual review of biochemistry.

[46]  J. Fargnoli,et al.  Decreased expression of heat shock protein 70 mRNA and protein after heat treatment in cells of aged rats. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[47]  Edward L. Schneider,et al.  Handbook of the Biology of Aging , 1990 .

[48]  S. Lindquist,et al.  The heat-shock proteins. , 1988, Annual review of genetics.