Exploration on Filamentous Phenotype of Coprinus comatus Colleted form Different Ecological Origins

Coprinus comatus is an edible and nematophagous basidiomycete fungus. The optimal culture conditions for the filamentous growth and density of 6 strains of this mushroom were investigated. The temperature suitable for the filamentous growth and density was obtained at 25 oC and optimal range of temperature was 20~30 oC. This mushroom showed a broad pH range (5~9) for its mycelial growth and density. The mostly favorable growth was found at pH 7. According to filamentous growth Czapek’s, PDA, YM, and Hamada were the most suitable, and Hennerberg and Hoppkins were the most unfavorable for this mushroom. Among 10 different carbon sources, sucrose and sorbitol were the best but lactose and xylose were the most unfavorable carbon sources. In all carbon sources, mycelial density was found to be compact. The most suitable nitrogen source was arginine and glycine but the most unsuitable was histidine for the mycelial growth. The mycelial density in nitrogen source containing medium was found to be different on the culture media.