A simple method for determining diazepam and its major metabolites in biological fluids: application in bioavailability studies.

: A simple gas chromatographic analytical method for determining diazepam and its major metabolites in the blood, urine, and muscle homogenate is described. Its applicability is illustrated by bioavailability studies, on man and on animals. The human studies revealed equivalence between two marketed brands of diazepam tablets and a slight difference between two brands of suppositories. The animal experiments showed agreement of in vivo properties of two different formulations of a diazepam injection fluid.

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