High‐Purity Prostate Circulating Tumor Cell Isolation by a Polymer Nanofiber‐Embedded Microchip for Whole Exome Sequencing

Handpick single cancer cells: a modified NanoVelcro Chip is coupled with ArcturusXT laser capture microdissection (LCM) technology to enable the detection and isolation of single circulating tumor cells (CTCs) from patients with prostate cancer (PC). This new approach paves the way for conducting next-generation sequencing (NGS) on single CTCs.

[1]  J. Chalmers,et al.  Optimization of an enrichment process for circulating tumor cells from the blood of head and neck cancer patients through depletion of normal cells , 2009, Biotechnology and bioengineering.

[2]  A W Partin,et al.  Natural history of progression after PSA elevation following radical prostatectomy. , 1999, JAMA.

[3]  H. Moch,et al.  Metastatic patterns of prostate cancer: an autopsy study of 1,589 patients. , 2000, Human pathology.

[4]  Steven J. M. Jones,et al.  Circos: an information aesthetic for comparative genomics. , 2009, Genome research.

[5]  I. Mezić,et al.  Chaotic Mixer for Microchannels , 2002, Science.

[6]  Robert A. Weinberg,et al.  Metastasis genes: A progression puzzle , 2002, Nature.

[7]  P. A. Futreal,et al.  Intratumor heterogeneity and branched evolution revealed by multiregion sequencing. , 2012, The New England journal of medicine.

[8]  Jocelyn Kaiser,et al.  Medicine. Cancer's circulation problem. , 2010, Science.

[9]  Nicholas Navin,et al.  Future medical applications of single-cell sequencing in cancer , 2011, Genome Medicine.

[10]  Hong Wu,et al.  Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells. , 2009, Angewandte Chemie.

[11]  Tejal A Desai,et al.  Biomimetic nanowire coatings for next generation adhesive drug delivery systems. , 2009, Nano letters.

[12]  Yun Wu,et al.  Broad Cross-Presentation of the Hematopoietically Derived PR1 Antigen on Solid Tumors Leads to Susceptibility to PR1-Targeted Immunotherapy , 2012, The Journal of Immunology.

[13]  Yi-Kuen Lee,et al.  Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers. , 2011, Angewandte Chemie.

[14]  D. Dearnaley,et al.  Characterization of ERG, AR and PTEN gene status in circulating tumor cells from patients with castration-resistant prostate cancer. , 2009, Cancer research.

[15]  Christos Sotiriou,et al.  Circulating tumor cells and emerging blood biomarkers in breast cancer , 2010, Current opinion in oncology.

[16]  Jim Kling,et al.  Beyond counting tumor cells , 2012, Nature Biotechnology.

[17]  A. Chambers,et al.  Detection and quantification of circulating tumor cells in mouse models of human breast cancer using immunomagnetic enrichment and multiparameter flow cytometry , 2005, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[18]  Hsian-Rong Tseng,et al.  Functionalized Conducting Polymer Nanodots for Enhanced Cell Capturing: The Synergistic Effect of Capture Agents and Nanostructures , 2011, Advanced materials.

[19]  Nada Jabado,et al.  Circulating tumor cells: detection, molecular profiling and future prospects , 2007, Expert review of proteomics.

[20]  Siyang Zheng,et al.  Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells. , 2007, Journal of chromatography. A.

[21]  L. Liotta,et al.  Laser capture microdissection: Arcturus(XT) infrared capture and UV cutting methods. , 2012, Methods in molecular biology.

[22]  Boran Cheng,et al.  Electrospun TiO2 Nanofiber‐Based Cell Capture Assay for Detecting Circulating Tumor Cells from Colorectal and Gastric Cancer Patients , 2012, Advanced materials.