A napin-like polypeptide with translation-inhibitory, trypsin-inhibitory, antiproliferative and antibacterial activities from kale seeds.

A heterodimeric napin-like polypeptide with translation-inhibiting and antibacterial activities has been isolated from kale seeds. The purification procedure entailed ion-exchange chromatography on dielthylaminoethyl (DEAE)-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and gel filtration by FPLC on Superdex 75. The napin-like polypeptide was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and Mono S. Its 7-kDa large subunit differs in N-terminal amino acid sequence from the 4-kDa small subunit. The polypeptide inhibited translation in the rabbit reticulocyte lysate system with an IC50 of 37.5 nM. This activity was preserved between pH 5 and pH 11, and between 10 and 40 degrees C. It fell to a low level at pH 3 and pH 13 and at 70 degrees C. Antibacterial activity against Bacillus, Megabacterium, and Pseudomonas species and antiproliferative activity against leukemia L1210 cells were observed. However, the polypeptide did not exert antifungal, ribonuclease, or protease activity.

[1]  T. Ng,et al.  A Homodimeric Sporamin-Type Trypsin Inhibitor with Antiproliferative, HIV Reverse Transcriptase-Inhibitory and Antifungal Activities from Wampee (Clausena lansium) Seeds , 2003, Biological chemistry.

[2]  T. Ng,et al.  Isolation and characterization of luffacylin, a ribosome inactivating peptide with anti-fungal activity from sponge gourd (Luffa cylindrica) seeds , 2002, Peptides.

[3]  T. Ng,et al.  Demonstration of antifungal and anti-human immunodeficiency virus reverse transcriptase activities of 6-methoxy-2-benzoxazolinone and antibacterial activity of the pineal indole 5-methoxyindole-3-acetic acid. , 2002, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[4]  T. Ng,et al.  Delandin, a chitinase-like protein with antifungal, HIV-1 reverse transcriptase inhibitory and mitogenic activities from the rice bean Delandia umbellata. , 2002, Protein expression and purification.

[5]  T. Ng,et al.  A new antifungal protein and a chitinase with prominent macrophage-stimulating activity from seeds of Phaseolus vulgaris cv. pinto. , 2002, Biochemical and biophysical research communications.

[6]  T. Ng,et al.  Luffangulin, a novel ribosome inactivating peptide from ridge gourd (Luffa acutangula) seeds. , 2002, Life sciences.

[7]  T. Ng,et al.  A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans. , 2001, Biochemical and biophysical research communications.

[8]  T. Ng,et al.  Isolation of a Homodimeric Lectin with Antifungal and Antiviral Activities from Red Kidney Bean (Phaseolus vulgaris) Seeds , 2001, Journal of protein chemistry.

[9]  T. Ng,et al.  Purification of Chrysancorin, a Novel Antifungal Protein with Mitogenic Activity from Garland Chrysanthemum Seeds , 2001, Biological chemistry.

[10]  T. Ng,et al.  Structurally dissimilar proteins with antiviral and antifungal potency from cowpea (Vigna unguiculata) seeds. , 2000, Life sciences.

[11]  T. Ng,et al.  Mungin, a novel cyclophilin-like antifungal protein from the mung bean. , 2000, Biochemical and biophysical research communications.

[12]  T. Ng,et al.  Sativin: a novel antifungal miraculin-like protein isolated from legumes of the sugar snap Pisum sativum var. macrocarpon. , 2000, Life sciences.

[13]  J. Barciszewski,et al.  Minireview: Analysis of Rape Seed Napin Structure and Potential Roles of the Storage Protein , 2000, Journal of protein chemistry.

[14]  T. Ng,et al.  Intrinsic ribonuclease activities in ribonuclease and ribosome-inactivating proteins from the seeds of bitter gourd. , 2000, The international journal of biochemistry & cell biology.

[15]  N. Raikhel,et al.  What do proteins need to reach different vacuoles? , 1999, Trends in plant science.

[16]  T. Ng,et al.  Purification and characterization of novel ribosome inactivating proteins, alpha- and beta-pisavins, from seeds of the garden pea Pisum sativum. , 1998, Biochemical and biophysical research communications.

[17]  M. de Virgilio,et al.  Sorting of Phaseolin to the Vacuole Is Saturable and Requires a Short C-Terminal Peptide , 1998, Plant Cell.

[18]  L. Mourey,et al.  Characterization and sugar-binding properties of arcelin-1, an insecticidal lectin-like protein isolated from kidney bean (Phaseolus vulgaris L. cv. RAZ-2) seeds. , 1998, The Biochemical journal.

[19]  G. Polya,et al.  Purification and sequencing of multiple forms of Brassica napus seed napin large chains that are calmodulin antagonists and substrates for plant calcium-dependent protein kinase. , 1996, Biochimica et biophysica acta.

[20]  L. Barbieri,et al.  Ribosome-inactivating proteins from plants. , 1993, Biochimica et biophysica acta.

[21]  G. Polya,et al.  Purification and Sequencing of Radish Seed Calmodulin Antagonists Phosphorylated by Calcium-Dependent Protein Kinase , 1993, Plant physiology.

[22]  E. Krebbers,et al.  Production of peptides in plant seeds. , 1990, Trends in biotechnology.