Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study

Purpose: To study the aggressiveness of peripheral giant cell lesion (PGCL) using hystochemical-immunohystochemical analysis. Methods: The archives of the Dental School of Aracatuba (FOA-UNESP) and the University of Passo Fundo Dental School (FOUPF) were searched. A total of 181 PGCL cases were retrieved, and 61 met the study eligibility criteria. Firstly, the PGCL aggressiveness was graded, and the lesions were distributed into three groups based on clinical and radiographic findings. The 61 LCGPs hystopathological samples were colored by H.E. and analyzed for diagnostic confirmation. Afterwards, the cellular proliferation of 15 PGCLs cases was investigated by means of counting and calibrating of AgNORs (nucleolar organizer regions) area and evaluation of the immunoexpression of PCNA, Ki-67, and p53 markers. Results: No difference was found for AgNOR area and PCNA expression among PGCLs of the three studied groups, and p53 was not an effective immunomarker of the analyzed lesions. There was a statistically significant difference of AgNORs number and Ki-67 expression among the three groups. Conclusion: The AgNOR and Ki-67 results suggest that the PGCL clinical-radiographic aggressiveness can be related to its cellular proliferative activity. Key words: Giant cell peripheric lesion; diagnosis; silver staining; immunohystochemistry

[1]  T. Lombardi,et al.  Post-traumatic development of a peripheral giant cell granuloma in a child. , 2008, Dental traumatology : official publication of International Association for Dental Traumatology.

[2]  B. Duarte Ruiz,et al.  Reparative giant cell granuloma in a pediatric patient. , 2007, Medicina oral, patologia oral y cirugia bucal.

[3]  M. H. Motamedi,et al.  Peripheral and central giant cell granulomas of the jaws: a demographic study. , 2007, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[4]  G. Sándor,et al.  An analysis of peripheral giant cell granuloma associated with dental implant treatment. , 2007, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[5]  M. Lozano,et al.  Granuloma periférico de células gigantes , 2006 .

[6]  U. Joos,et al.  Central giant cell granuloma of the jaws: a clinical, radiologic, and histopathologic study of 26 cases. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[7]  P. Jeannesson,et al.  Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level , 2005, The Histochemical Journal.

[8]  J. Wunder,et al.  Central giant cell granuloma of the jaws: assessment of cell cycle proteins. , 2004, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[9]  R. Boonstra,et al.  The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis , 2002, Journal of Neuroscience Methods.

[10]  R. Mesquita,et al.  Evaluation of p53, PCNA, Ki-67, MDM2 and AgNOR in oral peripheral and central giant cell lesions. , 2008, Oral diseases.

[11]  R. Gomez,et al.  Immunohistochemical expression of p53, MDM2, Ki-67 and PCNA in central giant cell granuloma and giant cell tumor. , 2007, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[12]  C. Flaitz,et al.  An aggressive peripheral giant cell granuloma in a child. , 1999, The Journal of clinical pediatric dentistry.

[13]  Y. Soini,et al.  Aberrant accumulation of p53 associates with Ki67 and mitotic count in benign skin lesions , 1994, The British journal of dermatology.

[14]  D. Lane,et al.  Proliferating cell nuclear antigen expression in non-cycling cells may be induced by growth factors in vivo. , 1994, British Journal of Cancer.

[15]  N. Vigneswaran,et al.  Giant cell lesions of the jaws: evaluation of nucleolar organizer regions in lesions of varying behavior. , 1993, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[16]  C. Waldron,et al.  Central giant cell lesions of the jaws. A clinical, radiologic, and histopathologic study. , 1993, Oral surgery, oral medicine, and oral pathology.

[17]  D. Lane,et al.  p53, guardian of the genome , 1992, Nature.

[18]  D. Lane,et al.  Cancer. p53, guardian of the genome. , 1992, Nature.

[19]  K. Plate,et al.  Nucleolar organizer regions (NORs). Basic concepts and practical application in tumor pathology. , 1989, Pathology, research and practice.

[20]  A. Perez-Atayde,et al.  Central giant cell lesions of the jaws: a clinicopathologic study. , 1986, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.