The influence of local pressure on evaluation parameters of skin blood perfusion and fluorescence

This article presents the results of the study of the pressure applied on optical diagnostic probes as a significant factor affecting the results of measurements. During stepwise increasing and decreasing of local pressure on skin we conducted measurements using the methods of laser Doppler flowmetry and fluorescence spectroscopy. It was found out that pressure on optical probe has sufficient impact on skin microcirculation to affect registered fluorescence intensity. Data obtained in this study are of interest for design and development of diagnostic technologies for wearable devices. This data will also inform further investigation into issues of compensation of blood absorption influence on fluorescence spectrum, allowing increased accuracy and reproducibility of measurements by fluorescence spectroscopy methods in optical diagnosis.

[1]  Vyacheslav Kalchenko,et al.  Label free in vivo laser speckle imaging of blood and lymph vessels. , 2012, Journal of biomedical optics.

[2]  I. Meglinski,et al.  Effect of mechanical pressure on the skin surface produced by fibre-optic probe in a blood microcirculation study , 2002 .

[3]  Victor V. Dremin,et al.  The development of attenuation compensation models of fluorescence spectroscopy signals , 2016, Saratov Fall Meeting.

[4]  F F de Mul,et al.  Principles and practice of the laser-Doppler perfusion technique. , 1999, Technology and health care : official journal of the European Society for Engineering and Medicine.

[5]  N. A. Stewart,et al.  Investigating tissue respiration and skin microhaemocirculation under adaptive changes and the synchronization of blood flow and oxygen saturation rhythms , 2014, Physiological measurement.

[6]  V. Tuchin.  Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis , 2000 .

[7]  N. A. Stewart,et al.  Individual variability analysis of fluorescence parameters measured in skin with different levels of nutritive blood flow. , 2015, Medical engineering & physics.

[8]  Igor Victorovich Meglinski,et al.  Skin blood microcirculation probing: experiments and theoretical remarks , 2002, Saratov Fall Meeting.

[9]  Vyacheslav Kalchenko,et al.  In vivo characterization of tumor and tumor vascular network using multi‐modal imaging approach , 2011, Journal of biophotonics.

[10]  S. Jacques.  Optical properties of biological tissues: a review , 2013, Physics in medicine and biology.

[11]  Vyacheslav Kalchenko,et al.  A simple approach for non‐invasive transcranial optical vascular imaging (nTOVI) , 2015, Journal of biophotonics.

[12]  M. Mycek,et al.  Handbook of Biomedical Fluorescence , 2003 .