On-line measurement of intracellular ATP of Saccharomyces cerevisiae and pyruvate during sake mashing.

The concentrations of intracellular ATP of Saccharomyces cerevisiae and pyruvate in a medium were instantaneously increased by pulse addition of glucose during starvation. They were reduced rapidly by alcohol fortification of the medium, accompanied by simultaneous increases of acetaldehyde concentration and inviability of yeast cells. These results were monitored during fermentation of sake mash by an on-line measuring method. Intracellular ATP and pyruvate concentrations were considered to be indicators of the physiological state of the yeast in sake mash. During sake mashing, it was observed that an increase in temperature enhanced the intracellular ATP concentration and the pyruvate production of the yeast. Since pyruvate production was not affected intensely by changes in temperature during cultivation in a glucose-limited chemostat, this effect was thought to be due to the enhanced rates of cell-growth and/or alcohol production. This suggests that the control of mashing temperature during cell growth until about 10% alcohol accumulation is achieved is important for the control of the pyruvate concentration in sake mash.

[1]  A. Humphrey,et al.  Use of fluorometry for monitoring and control of a bioreactor , 1991, Biotechnology and bioengineering.

[2]  M. Rigoulet,et al.  Interactions between glucose metabolism and oxidative phosphorylations on respiratory-competent Saccharomyces cerevisiae cells. , 1993, European journal of biochemistry.

[3]  C. Ratledge,et al.  Yeast physiology — a micro-synopsis , 1991 .

[4]  R. Jones.  Factors influencing deactivation of yeast cells exposed to ethanol. , 1987, The Journal of applied bacteriology.

[5]  K. Sigler,et al.  Mechanisms of acid extrusion in yeast. , 1991, Biochimica et biophysica acta.

[6]  Ian W. Marison,et al.  Metabolic uncoupling in Saccharomyces cerevisiae , 1995 .

[7]  M. Reuss,et al.  In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae : I. Experimental observations. , 1997, Biotechnology and bioengineering.

[8]  M. Reuss,et al.  In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae: II. Mathematical model. , 1997, Biotechnology and bioengineering.

[9]  M. Pareja,et al.  Changes in the intracellular concentrations of the adenosine phosphates and nicotinamide adenine dinucleotides ofSaccharomyces cerevisiae during batch fermentation , 1995, World journal of microbiology & biotechnology.

[10]  Kazuo Satô,et al.  A new sampling means for on-line analysis of brewing mash , 1998 .

[11]  J Villadsen,et al.  Physiological effects of nitrogen starvation in an anaerobic batch culture of Saccharomyces cerevisiae. , 1996, Microbiology.

[12]  Hans V. Westerhoff,et al.  Synchrony and mutual stimulation of yeast cells during fast glycolytic oscillations , 1992 .

[13]  L. Ingram,et al.  Intracellular accumulation of AMP as a cause for the decline in rate of ethanol production by Saccharomyces cerevisiae during batch fermentation , 1988, Applied and environmental microbiology.

[14]  K. Schügerl,et al.  Culture fluorescence studies on aerobic continuous cultures ofSaccharomyces cerevisiae , 1986, Applied Microbiology and Biotechnology.

[15]  Rodney P. Jones,et al.  Biological principles for the effects of ethanol , 1989 .

[16]  W. A. Scheffers,et al.  Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. , 1990, Journal of general microbiology.

[17]  N. Uden,et al.  Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae , 1987, Applied and environmental microbiology.

[18]  M. Reuss,et al.  In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique. , 1993, Analytical biochemistry.

[19]  J. W. Hastings.  [13] Bacterial bioluminescence: An overview , 1978 .

[20]  J. Pronk,et al.  Pyruvate Metabolism in Saccharomyces cerevisiae , 1996, Yeast.

[21]  Kazuo Sato,et al.  Measuring and controlling in Sake making process. Part 2 On-line monitoring of ethanol and specific gravity in Sake mash.:Measuring and Controlling in Sake Making Process (Part 2) , 1986 .

[22]  Yukio Kawaguchi,et al.  Immobilization of Nitrifying Bacteria by Polyethylene Glycol Prepolymer , 1992 .

[23]  R. Mutharasan,et al.  NADH and flavin fluorescence responses of starved yeast cultures to substrate additions , 1989, Biotechnology and bioengineering.

[24]  L. Ingram,et al.  Regulation of Glycolytic Flux and Ethanol Production in Saccharomyces cerevisiae: Effects of Intracellular Adenine Nucleotide Concentrations on the In Vitro Activities of Hexokinase, Phosphofructokinase, Phosphoglycerate Kinase, and Pyruvate Kinase , 1989, Applied and environmental microbiology.

[25]  M. Sami,et al.  Evaluation of the alkaline methylene blue staining method for yeast activity determination , 1994 .

[26]  J. E. Hallsworth.  Ethanol-induced water stress in yeast , 1998 .

[27]  D. Zabriskie,et al.  THE INTERPRETATION OF ON-LINE PROCESS MEASUREMENTS OF INTRACELLULAR NADH IN FERMENTATION PROCESSES , 1986 .

[28]  L. Ingram,et al.  Ethanol production during batch fermentation with Saccharomyces cerevisiae: changes in glycolytic enzymes and internal pH , 1987, Applied and environmental microbiology.

[29]  E. Querfurth,et al.  In vivo investigations of glucose transport in Saccharomyces cerevisiae , 2000, Biotechnology and bioengineering.

[30]  H. Schneckenburger,et al.  Oxygen concentration and the oxidation-reduction state of yeast: Determination of free/bound NADH and flavins by time-resolved spectroscopy , 2005, Naturwissenschaften.

[31]  W. A. Scheffers,et al.  Metabolic responses of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 upon transition from glucose limitation to glucose excess , 1988, Yeast.

[32]  W. A. Scheffers,et al.  Energetics of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. , 1990, Journal of general microbiology.

[33]  D. E. Atkinson,et al.  Adenylate energy charge in Saccharomyces cerevisiae during starvation , 1975, Journal of bacteriology.

[34]  G Stephanopoulos,et al.  Physiological, biochemical, and mathematical studies of micro‐aerobic continuous ethanol fermentation by Saccharomyces cerevisiae. II: Intracellular metabolite and enzyme assays at steady state chemostat cultures , 1990, Biotechnology and bioengineering.

[35]  D. White,et al.  Fluorometric Determination of Adenosine Nucleotide Derivatives as Measures of the Microfouling, Detrital, and Sedimentary Microbial Biomass and Physiological Status , 1980, Applied and environmental microbiology.