Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants

[1]  C. Wood,et al.  A Synthetic Biology Workflow Reveals Variation in Processing and Solubility of Nitrogenase Proteins Targeted to Plant Mitochondria, and Differing Tolerance of Targeting Sequences in a Bacterial Nitrogenase Assay , 2020, Frontiers in Plant Science.

[2]  C. Wood,et al.  Plant expression of NifD protein variants resistant to mitochondrial degradation , 2020, Proceedings of the National Academy of Sciences.

[3]  R. Dixon,et al.  Using synthetic biology to overcome barriers to stable expression of nitrogenase in eukaryotic organelles , 2020, Proceedings of the National Academy of Sciences.

[4]  L. Seefeldt,et al.  Reduction of Substrates by Nitrogenases. , 2020, Chemical reviews.

[5]  E. Caro,et al.  Use of synthetic biology tools to optimize the production of active nitrogenase Fe protein in chloroplasts of tobacco leaf cells , 2020, Plant biotechnology journal.

[6]  Stefan Burén,et al.  Biosynthesis of Nitrogenase Cofactors , 2020, Chemical reviews.

[7]  Sarah E. Bloch,et al.  Harnessing atmospheric nitrogen for cereal crop production. , 2019, Current opinion in biotechnology.

[8]  Christopher A. Voigt,et al.  Biosynthesis of the nitrogenase active-site cofactor precursor NifB-co in Saccharomyces cerevisiae , 2019, Proceedings of the National Academy of Sciences.

[9]  S. Haruta,et al.  Nitrogenase Activity in Thermophilic Chemolithoautotrophic Bacteria in the Phylum Aquificae Isolated under Nitrogen-Fixing Conditions from Nakabusa Hot Springs , 2018, Microbes and environments.

[10]  Ray Dixon,et al.  Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology , 2018, Proceedings of the National Academy of Sciences.

[11]  L. Seefeldt,et al.  Sequential and differential interaction of assembly factors during nitrogenase MoFe protein maturation , 2018, The Journal of Biological Chemistry.

[12]  P. Bénit,et al.  Mitochondria are physiologically maintained at close to 50 ̊C , 2018 .

[13]  Stefan Burén,et al.  State of the art in eukaryotic nitrogenase engineering , 2017, FEMS microbiology letters.

[14]  Stefan Burén,et al.  Diversity and Functional Analysis of the FeMo-Cofactor Maturase NifB , 2017, Front. Plant Sci..

[15]  Matin Qaim,et al.  Rural food security, subsistence agriculture, and seasonality , 2017, PloS one.

[16]  Stefan Burén,et al.  Purification and In Vitro Activity of Mitochondria Targeted Nitrogenase Cofactor Maturase NifB , 2017, Front. Plant Sci..

[17]  Christopher A. Voigt,et al.  Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae , 2017, ACS synthetic biology.

[18]  L. M. Rubio,et al.  The Nitrogenase FeMo-Cofactor Precursor Formed by NifB Protein: A Diamagnetic Cluster Containing Eight Iron Atoms. , 2016, Angewandte Chemie.

[19]  L. M. Rubio,et al.  Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast , 2016, Nature Communications.

[20]  E. Koonin.  Origin of eukaryotes from within archaea, archaeal eukaryome and bursts of gene gain: eukaryogenesis just made easier? , 2015, Philosophical Transactions of the Royal Society B: Biological Sciences.

[21]  Allison M. Leach,et al.  Nitrogen: too much of a vital resource , 2015 .

[22]  Essi V. Koskela,et al.  Homologous Recombinatorial Cloning Without the Creation of Single-Stranded Ends: Exonuclease and Ligation-Independent Cloning (ELIC) , 2015, Molecular Biotechnology.

[23]  Dennis R. Dean,et al.  Mechanism of Nitrogen Fixation by Nitrogenase: The Next Stage , 2014, Chemical reviews.

[24]  Carlos Echavarri-Erasun,et al.  Kinetics of nif Gene Expression in a Nitrogen-Fixing Bacterium , 2013, Journal of bacteriology.

[25]  Wirth,et al.  Complete genome sequence of Hydrogenobacter thermophilus type strain (TK-6) , 2014 .

[26]  Uwe Carl,et al.  Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants. , 2013, Protein expression and purification.

[27]  R. Hellens,et al.  Advanced Engineering of Lipid Metabolism in Nicotiana benthamiana Using a Draft Genome and the V2 Viral Silencing-Suppressor Protein , 2012, PloS one.

[28]  Christopher A. Voigt,et al.  Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca , 2012, Proceedings of the National Academy of Sciences.

[29]  Loredano Pollegioni,et al.  Molecular basis of glyphosate resistance – different approaches through protein engineering , 2011, The FEBS journal.

[30]  Lynne A. Goodwin,et al.  Complete genome sequence of Hydrogenobacter thermophilus type strain (TK-6T) , 2011, Standards in genomic sciences.

[31]  N. Pfanner,et al.  Global Analysis of the Mitochondrial N-Proteome Identifies a Processing Peptidase Critical for Protein Stability , 2009, Cell.

[32]  L. Curatti,et al.  In vitro synthesis of the iron–molybdenum cofactor of nitrogenase from iron, sulfur, molybdenum, and homocitrate using purified proteins , 2007, Proceedings of the National Academy of Sciences.

[33]  L. Curatti,et al.  NifX and NifEN exchange NifB cofactor and the VK‐cluster, a newly isolated intermediate of the iron‐molybdenum cofactor biosynthetic pathway , 2007, Molecular microbiology.

[34]  N. Borlaug.  Feeding a world of 10 billion people: The miracle ahead , 2002, In Vitro Cellular & Developmental Biology - Plant.

[35]  Arthur Schweiger,et al.  EasySpin, a comprehensive software package for spectral simulation and analysis in EPR. , 2006, Journal of magnetic resonance.

[36]  E. Hinchliffe,et al.  Improving the nutritional value of Golden Rice through increased pro-vitamin A content , 2005, Nature Biotechnology.

[37]  D. Dean,et al.  The nifU, nifS and nifV gene products are required for activity of all three nitrogenases of Azotobacter vinelandii , 1992, Molecular and General Genetics MGG.

[38]  W. Neupert,et al.  Mitochondria‐targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae , 2000, Yeast.

[39]  Toshimichi Ikemura,et al.  Codon usage tabulated from international DNA sequence databases: status for the year 2000 , 2000, Nucleic Acids Res..

[40]  W. Lanzilotta,et al.  Catalytic and biophysical properties of a nitrogenase Apo-MoFe protein produced by a nifB-deletion mutant of Azotobacter vinelandii. , 1998, Biochemistry.

[41]  A. Varshavsky,et al.  Yeast N-terminal Amidase , 1995, The Journal of Biological Chemistry.

[42]  D. Dean,et al.  nifU gene product from Azotobacter vinelandii is a homodimer that contains two identical [2Fe-2S] clusters. , 1994, Biochemistry.

[43]  D. Dean,et al.  Catalytic formation of a nitrogenase iron-sulfur cluster. , 1994, The Journal of biological chemistry.

[44]  R. Setterquist,et al.  Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii , 1989, Journal of bacteriology.

[45]  H. Hill,et al.  Protein determination using bicinchoninic acid in the presence of sulfhydryl reagents. , 1988, Analytical biochemistry.

[46]  W. Fish,et al.  Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples. , 1988, Methods in enzymology.

[47]  W. Orme-Johnson,et al.  Klebsiella pneumoniae nifM gene product is required for stabilization and activation of nitrogenase iron protein in Escherichia coli. , 1986, The Journal of biological chemistry.

[48]  D. Emerich,et al.  Complementary functioning of the component proteins of nitrogenase from several bacteria , 1978, Journal of bacteriology.

[49]  K Winter,et al.  [The next stage]. , 1974, Zeitschrift fur arztliche Fortbildung.

[50]  W. Brill,et al.  Nitrogenase. IV. Simple method of purification to homogeneity of nitrogenase components from Azotobacter vinelandii. , 1973, Biochimica et biophysica acta.

[51]  W. A. Bulen,et al.  The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis. , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[52]  George Gallup.  The miracle ahead , 1964 .