OXA b-lactamases in Acinetobacter: the story so far

The emergence of carbapenem resistance inAcinetobacter baumannii has become a global concern since these b-lactams are often the only effective treatment left against many multiresistant strains. A recent development has been the discovery of a novel group of narrow-spectrum OXA b-lactamases in carbapenem-resistant strains, some of which have acquired the ability to hydrolyse the carbapenems. The first of these was found in a strain isolated in Edinburgh before imipenem was in use in the hospital. Whether these carbapenemases have been acquired or are part of the genetic make-up of this species has yet to be determined. More importantly, however, they represent an important stage in the evolution of antibiotic resistance inAcinetobacter. Thispaperdiscusses theemergenceof theseunusual enzymesover the past decade.

[1]  A. Oliver,et al.  OXA-24, a Novel Class D β-Lactamase with Carbapenemase Activity in an Acinetobacter baumannii Clinical Strain , 2006, Antimicrobial Agents and Chemotherapy.

[2]  N. Woodford,et al.  Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. , 2006, International journal of antimicrobial agents.

[3]  J. Claverie,et al.  Characterization of the Naturally Occurring Oxacillinase of Acinetobacter baumannii , 2005, Antimicrobial Agents and Chemotherapy.

[4]  S. Amyes,et al.  The sequences of seven class D beta-lactamases isolated from carbapenem-resistant Acinetobacter baumannii from four continents. , 2005, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[5]  P. Nordmann,et al.  OXA-58, a Novel Class D β-Lactamase Involved in Resistance to Carbapenems in Acinetobacter baumannii , 2005, Antimicrobial Agents and Chemotherapy.

[6]  Qing Yang,et al.  Typing and characterization of carbapenem-resistant Acinetobacter calcoaceticus-baumannii complex in a Chinese hospital. , 2004, Journal of medical microbiology.

[7]  P. Nordmann,et al.  Genetic and Biochemical Characterization of a Chromosome-Encoded Carbapenem-Hydrolyzing Ambler Class D β-Lactamase from Shewanella algae , 2004, Antimicrobial Agents and Chemotherapy.

[8]  P. Villari,et al.  Molecular Epidemiology of Sequential Outbreaks of Acinetobacter baumannii in an Intensive Care Unit Shows the Emergence of Carbapenem Resistance , 2004, Journal of Clinical Microbiology.

[9]  J. Vila,et al.  Type 1 Integrons in Epidemiologically Unrelated Acinetobacter baumannii Isolates Collected at Spanish Hospitals , 2004, Antimicrobial Agents and Chemotherapy.

[10]  P. Nordmann,et al.  Chromosome-Encoded Ambler Class D β-Lactamase of Shewanella oneidensis as a Progenitor of Carbapenem-Hydrolyzing Oxacillinase , 2004, Antimicrobial Agents and Chemotherapy.

[11]  N. Woodford,et al.  Outbreak of Carbapenem-Resistant Acinetobacter baumannii Producing the OXA-23 Enzyme in Curitiba, Brazil , 2003, Journal of Clinical Microbiology.

[12]  P. Nordmann,et al.  Genetic and Functional Analysis of the Chromosome-Encoded Carbapenem-Hydrolyzing Oxacillinase OXA-40 of Acinetobacter baumannii , 2003, Antimicrobial Agents and Chemotherapy.

[13]  N. Woodford,et al.  Endemic Carbapenem Resistance Associated with OXA-40 Carbapenemase among Acinetobacter baumannii Isolates from a Hospital in Northern Spain , 2002, Journal of Clinical Microbiology.

[14]  P. Nordmann,et al.  Prospective Survey of β-Lactamases Produced by Ceftazidime- Resistant Pseudomonas aeruginosa Isolated in a French Hospital in 2000 , 2002, Antimicrobial Agents and Chemotherapy.

[15]  P. Hsueh,et al.  Pandrug-Resistant Acinetobacter baumannii Causing Nosocomial Infections in a University Hospital, Taiwan , 2002, Emerging infectious diseases.

[16]  R. Bonnet,et al.  Chromosome-Encoded Class D β-Lactamase OXA-23 in Proteus mirabilis , 2002, Antimicrobial Agents and Chemotherapy.

[17]  N. Woodford,et al.  Characterization of OXA-25, OXA-26, and OXA-27, Molecular Class D β-Lactamases Associated with Carbapenem Resistance in Clinical Isolates of Acinetobacter baumannii , 2001, Antimicrobial Agents and Chemotherapy.

[18]  M. Ploy,et al.  Molecular Characterization of Integrons inAcinetobacter baumannii: Description of a Hybrid Class 2 Integron , 2000, Antimicrobial Agents and Chemotherapy.

[19]  G. Bou,et al.  Characterization of a Nosocomial Outbreak Caused by a Multiresistant Acinetobacter baumannii Strain with a Carbapenem-Hydrolyzing Enzyme: High-Level Carbapenem Resistance inA. baumannii Is Not Due Solely to the Presence of β-Lactamases , 2000, Journal of Clinical Microbiology.

[20]  S. Amyes,et al.  Sequence Analysis of ARI-1, a Novel OXA β-Lactamase, Responsible for Imipenem Resistance inAcinetobacter baumannii 6B92 , 2000, Antimicrobial Agents and Chemotherapy.

[21]  P. Nordmann,et al.  Molecular Characterization of OXA-20, a Novel Class D β-Lactamase, and Its Integron from Pseudomonas aeruginosa , 1998, Antimicrobial Agents and Chemotherapy.

[22]  A. Vessières,et al.  Oxacillin-hydrolyzing beta-lactamase involved in resistance to imipenem in Acinetobacter baumannii. , 1997, FEMS microbiology letters.

[23]  E Bergogne-Bérézin,et al.  Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features , 1996, Clinical microbiology reviews.

[24]  S. Amyes,et al.  Transferable imipenem-resistance in Acinetobacter species from a clinical source. , 1995, The Journal of antimicrobial chemotherapy.

[25]  F. Sanschagrin,et al.  Primary structure of OXA-3 and phylogeny of oxacillin-hydrolyzing class D beta-lactamases , 1995, Antimicrobial agents and chemotherapy.

[26]  B. Kreiswirth,et al.  Clinical and molecular epidemiology of acinetobacter infections sensitive only to polymyxin B and sulbactam , 1994, The Lancet.

[27]  B. Berger,et al.  Effect of sulbactam on infections caused by imipenem-resistant Acinetobacter calcoaceticus biotype anitratus. , 1993, The Journal of infectious diseases.

[28]  R. Levesque,et al.  Phylogeny of LCR‐1 and OXA‐5 with class A and class D β‐lactamases , 1992, Molecular microbiology.

[29]  P. Nordmann,et al.  A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58. , 2005, The Journal of antimicrobial chemotherapy.

[30]  S. Amyes,et al.  Characterisation of OXA-51, a novel class D carbapenemase found in genetically unrelated clinical strains of Acinetobacter baumannii from Argentina. , 2005, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[31]  J. Ruiz,et al.  Characterization of an integron carrying a new class D beta-lactamase (OXA-37) in Acinetobacter baumannii. , 2002, Microbial drug resistance.